Jurkat whole cell lysate suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
Images
Publications
- Frontiers in molecular biosciences 8:6867182021Upregulation of Translationally Controlled Tumor Protein Is Associated With Cervical Cancer Progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyu Zhu,Ji Ren,Dianqin Xu,Di Cheng,Wei Wang,Jie Ren,Ziwen Xiao,Hongmei Jiang,Yan Ding,Yujie TanPubMed 34589516
- Biology of sex differences 11:122020Neuroprotective and neurotoxic outcomes of androgens and estrogens in an oxidative stress environment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhong Duong,Mavis A A Tenkorang,Jenny Trieu,Clayton McCuiston,Nataliya Rybalchenko,Rebecca L CunninghamPubMed 32223745
Key facts
- Cell type
Jurkat
- Species or organism
Human
- Tissue
Blood
- Form
Liquid
Associated Products
Select an associated product type
5 products for Alternative Product
Recommended products
Jurkat whole cell lysate suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
Key facts
- Cell type
Jurkat
- Species or organism
Human
- Tissue
Blood
- Form
Liquid
- Disease
Non-Hodgkin Lymphoma
- Concentration
Cell culture
- Biosafety level
EU: 1 US: 1
- Adherent/suspension
Suspension
- Gender
Male
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Cell line: Jurkat, clone E6-1 (Human acute T cell leukemia).
Growth media: RPMI and 10% NCS (Newborn calf serum).
Jurkat cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodiumchloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (0.045 M Tris-HCl pH 6.8, 10% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue), containing 0.05 M DTT.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
39 product images
Western blot - Jurkat whole cell lysate (ab7899)
ab1647 recognises a band of approximately the expected size (~53 kDa) for p53 phospho Serine 315 in Hela cell lysate but not in Embryonic Stem Cell or Jurkat Cell lysate. It was possible to block the band using the immunising peptide (Human p53 (phospho S315) peptide ab28896) but it was not possible to block the band using the unmodified version of the immunising peptide (ab28897). This suggests the ab1647 does not recognise unmodified p53. The identity of the band at ~40 kDa is unknown but it could be a p53 isoform.
All lanes: Anti-p53 (phospho S315) antibody (ab1647) at 1 µg/mL
Lanes 1, 4 and 7: Human Embryonic Stem Cell Lysate at 20 µg
Lanes 2, 5 and 8: Hela lysate at 20 µg
Lanes 3, 6 and 9: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
SecondaryAll lanes: Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-eIF3e antibody (Anti-eIF3e antibody ab36766) at 1 µg/mL
Lanes 1 and 5: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lanes 2 and 6: Western blot - Jurkat whole cell lysate (ab7899) at 10 µg
Lanes 3 and 7: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 10 µg
Lanes 4 and 8: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 40 kDa, 48 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Anti-Histone H2B antibody (ab18977) at 1 µg/mL
All lanes: Western blot - Jurkat whole cell lysate (ab7899)
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-VEGFA antibody (Anti-VEGFA antibody ab46154) at 0.5 µg/mL
Lanes 1 and 8: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lanes 2 and 9: Western blot - Jurkat whole cell lysate (ab7899) at 10 µg
Lanes 10 and 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 10 µg
Lanes 11 and 4: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 10 µg
Lanes 12 and 5: Hep G2 whole cell lysate (ab7900) at 10 µg
Lanes 13 and 6: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lanes 14 and 7: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-TIGAR antibody (Anti-TIGAR antibody ab37910) at 1/250 dilution
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 10 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 10 µg
Lane 4: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 10 µg
Lane 5: Hep G2 whole cell lysate (ab7900) at 10 µg
Lane 6: MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-BIN1 antibody (Anti-BIN1 antibody ab27796) at 2 µg/mL
Lane 1: Western blot - Jurkat whole cell lysate (ab7899)
Lane 2: Jurkat cells treated with camptothecin for 6 hours
Predicted band size: 65 kDa
Observed band size: 48 kDa, 64 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-eIF4A1 antibody (Anti-eIF4A1 antibody ab31217) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 47 kDa
Western blot - Jurkat whole cell lysate (ab7899)
The identification of the 55 kDa band is unclear but this band has also been observed in HeLa lysates in the Western Blot of Anti-Argonaute-2 antibody ab5072, targeting Drosophila Ago2 / eIF2C2.
All lanes: Western blot - Anti-Argonaute-2 antibody (Anti-Argonaute-2 antibody ab32381) at 2 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
Lane 3: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 55 kDa, 87 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-HMGB1 antibody (Anti-HMGB1 antibody ab18256) at 1 µg/mL
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 10 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 10 µg
Lane 4: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 29 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-CENPB antibody (Anti-CENPB antibody ab25734) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 65 kDa
Observed band size: 65 kDa, 74 kDa, 75 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (Anti-KDM1/LSD1 antibody - Nuclear Marker ab17721) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
Lane 3: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 92 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-Cytokeratin 18 antibody (Anti-Cytokeratin 18 antibody ab24561) at 1 µg/mL
Lane 1: HeLa whole cell lysate at 20 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Western blot - Jurkat whole cell lysate (ab7899)
This antibody detects a band at approximately 88kDa that corresponds in size to ZW10. It also detects bands at 75 and 45kDa that may be degradation products or crossreactivity. All of these bands are competed away by the addition of the immunizing peptide, suggesting that they are specific interactions.
All lanes: Western blot - Anti-ZW10 antibody (Anti-ZW10 antibody ab21582) at 1 µg/mL
All lanes: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG(Alexa Fluor® 680) at 1/10,000 dilution
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 45 kDa, 75 kDa, 88 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-DNA Polymerase beta antibody (Anti-DNA Polymerase beta antibody ab26343) at 0.5 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 150 kDa, 38 kDa, 48 kDa, 90 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-eIF4A2 antibody (Anti-eIF4A2 antibody ab31218) at 1 µg/mL
All lanes: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
SecondaryAll lanes: IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 47 kDa, 52 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-Prohibitin antibody - Mitochondrial Marker (Anti-Prohibitin antibody - Mitochondrial Marker ab28172) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-Nucleolin antibody (Anti-Nucleolin antibody ab22758)
Lanes 2 and 4: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
Lanes 3 and 5: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 100 kDa
Western blot - Jurkat whole cell lysate (ab7899)
The membrane 1-5 was blocked in 5% milk (1 hour). The membrane 6-10 was blocked in 5% BSA (1 hour). Milk is not a suitable blocking agent and significantly decreases the signal on the membrane.
All lanes: Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484) at 1/1000 dilution
Lanes 1 and 6: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg
Lanes 2 and 7: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lanes 3 and 8: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
Lanes 4 and 9: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
Lanes 10 and 5: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 20 µg
SecondaryAll lanes: Goat anti-Mouse (HRP conjugated) at 1/5000 dilution
Predicted band size: 36 kDa
Observed band size: 40 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-SUFU antibody (Anti-SUFU antibody ab28083) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 37 kDa, 54 kDa
Western blot - Jurkat whole cell lysate (ab7899)
An additional band at approximately 34 kDa is occasionally observed with Anti-Cdk9 antibody ab38840. This is thought to be due to cross-reactivity.
All lanes: Western blot - Anti-Cdk9 antibody (Anti-Cdk9 antibody ab38840) at 1/250 dilution
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 10 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 10 µg
Lane 4: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 140 kDa, 260 kDa, 41 kDa
Western blot - Jurkat whole cell lysate (ab7899)
Lane 1: Marker
Lanes 2 - 5: Western blot - Anti-hnRNP K antibody [F45 P9 C7] (Anti-hnRNP K antibody [F45 P9 C7] ab23644) at 1 µg/mL
Lane 2: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab27251) at 20 µg
Lane 3: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µg
Lane 4: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
Lane 5: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 20 µg
SecondaryAll lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (Rabbit Anti-Mouse IgG H&L (HRP) ab6728) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Western blot - Jurkat whole cell lysate (ab7899)
Although the predicted band size is 53kDa based on Swiss-prot data, a band of 65kDa has been previously observed. J Biol Chem. 2004 Nov 26;279(48):49773-9 (PMID: 15377657)
All lanes: Western blot - Anti-U2AF65 antibody (Anti-U2AF65 antibody ab37530) at 1/250 dilution
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 10 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 10 µg
Lane 4: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 10 µg
Lane 5: Hep G2 whole cell lysate (ab7900) at 10 µg
Lane 6: MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 7: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
Lane 8: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 65 kDa
Western blot - Jurkat whole cell lysate (ab7899)
The predicted band size is 48kDa based on Swiss-prot data, however a band of 55kDa is consistent with that observed in other commercially available antibodies to Calreticulin. This difference in size is likely to be the result of post-translational modification.All lanes: Western blot - Anti-Calreticulin antibody - ER Marker (Anti-Calreticulin antibody - ER Marker ab39897) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 10 µg
Lane 3: Hep G2 whole cell lysate (ab7900) at 10 µg
Lane 4: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 55 kDa, 75 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-eEF1B2 antibody (Anti-eEF1B2 antibody ab37725) at 1/250 dilution
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 10 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 10 µg
Lane 4: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 10 µg
Lane 5: Hep G2 whole cell lysate (ab7900) at 10 µg
Lane 6: MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 7: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 30 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-Rad6 antibody (Anti-Rad6 antibody ab31917) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
Lane 3: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H&L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 17 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-eRF1 antibody (Anti-eRF1 antibody ab31799) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 50 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-IMPDH1 antibody (Anti-IMPDH1 antibody ab33039) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat anti-rabbit IgG (H&L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Western blot - Jurkat whole cell lysate (ab7899)
Anti-FOXO3A antibody ab23683 detects a band at approximately 90 kDa that corresponds in size to that seen for FOXO3A. This protein has been shown to migrate at a size larger than the predicted molecular weight (see Yin et al., J. Biol. Chem., Vol. 279, Issue 44, 45721-45727). It also detects a band at 50 kDa which could be a cleavage fragment. Both bands are partially blocked by the addition of the immunizing peptide.
All lanes: Western blot - Anti-FOXO3A antibody (Anti-FOXO3A antibody ab23683) at 1 µg/mL
All lanes: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
Predicted band size: 71 kDa
Observed band size: 50 kDa, 90 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-EEF2/Elongation factor 2 antibody (Anti-EEF2/Elongation factor 2 antibody ab33523) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 10 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 10 µg
Lane 4: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 10 µg
Lane 5: Hep G2 whole cell lysate (ab7900) at 10 µg
Lane 6: MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 7: SHSY-5Y (Human Neuroblastoma) Whole Cell Lysate at 10 µg
Lane 8: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 95 kDa
Observed band size: 95 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-Menin antibody - ChIP Grade (Anti-Menin antibody - ChIP Grade ab31902) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 10 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 10 µg
Lane 4: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 31 kDa, 75 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-UBE2I / UBC9 antibody (Anti-UBE2I / UBC9 antibody ab33044) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
SecondaryAll lanes: IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Western blot - Jurkat whole cell lysate (ab7899)
Anti-SMC1A antibody ab21583 detects a band of 150 kDa, corresponding to the size of SMC1A. This band is competed away by the addition of the immunizing peptide, showing that it is a specific interaction.
All lanes: Western blot - Anti-SMC1A antibody (Anti-SMC1A antibody ab21583) at 1 µg/mL
Lanes 1 and 3: 20ug HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lanes 2 and 4: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 143 kDa
Observed band size: 150 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-POLD2 antibody (Anti-POLD2 antibody ab38338) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
Lane 3: Western blot - Jurkat whole cell lysate (ab7899) at 10 µg
Lane 4: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 10 µg
Lane 5: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 10 µg
Lane 6: Hep G2 whole cell lysate (ab7900) at 10 µg
Lane 7: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 48 kDa, 51 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-eRF1 antibody (Anti-eRF1 antibody ab30928) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Western blot - Jurkat whole cell lysate (ab7899)
SF2 is extensively phosphorylated on serine residues in the RS domain (SwissProt).
Anti-SF2 antibody ab38017 is targeted against all isoforms of the SF2 protein.
All lanes: Western blot - Anti-SF2 antibody (Anti-SF2 antibody ab38017) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 10 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 10 µg
Lane 4: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 10 µg
Lane 5: Hep G2 whole cell lysate (ab7900) at 10 µg
Lane 6: MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 7: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 34 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-XLF antibody (Anti-XLF antibody ab33499) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 37 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-RNF20 antibody (Anti-RNF20 antibody ab32629) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 10 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 10 µg
Lane 4: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 10 µg
Lane 5: MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 6: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 120 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-PRP19 antibody (Anti-PRP19 antibody ab27692) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - Jurkat whole cell lysate (ab7899) at 20 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
SecondaryAll lanes: IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Western blot - Jurkat whole cell lysate (ab7899)
All lanes: Western blot - Anti-RBBP4 antibody (Anti-RBBP4 antibody ab38135) at 1/250 dilution
Lane 1: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
Lane 2: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3: Western blot - Jurkat whole cell lysate (ab7899) at 10 µg
Lane 4: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 10 µg
Lane 5: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 10 µg
Lane 6: Hep G2 whole cell lysate (ab7900) at 10 µg
Lane 7: MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 100 kDa, 49 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com