AB14863
MCF7 nuclear extract lysate (H2O2 post treated)
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MCF7 nuclear extract lysate (H2O2 post treated). View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
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Functional Studies - MCF7 nuclear extract lysate (H2O2 post treated) (AB14863)
Each lot has been tested for Sp1 activity by using the TransAM™ Sp1 Kit. The signal intensity for Sp1 activity in each lot is compared to the signal intensity obtained with H2O2-post treated extracts from MCF-7 cells. Once the signals are blanked, the ratio between the signals from untreated extracts over treated extracts used at 5 μg/well must be above 4.
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Product details
Post-collection treatment consists in exposing the nuclear extract to a final concentration of 200 mM H2O2 for 20 min on ice. The protein content has been determined by a Bradford-based assay.
Properties and storage information
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Female
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