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AB94302

MCM4 overexpression 293T lysate (whole cell)

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MCM4 overexpression 293T lysate (whole cell) suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

View Alternative Names

19G, AI325074, AU045576, CDC 21, CDC 54, CDC21 homolog, CDC21, S. pombe, homolog of, Cell division cycle 21, S. pombe, homolog of, DNA replication licensing factor MCM 4, Homolog of S. pombe cell devision cycle 21, KIAA4003, MCM 4 minichromosome maintenance deficient 4, MCM4 minichromosome maintenance deficient 4, mitotin (S. cerevisiae), MCM4_HUMAN, MGC33310, Mcmd4, Minichromosome maintenance 4, Minichromosome maintenance complex component 4, Minichromosome maintenance deficient (S. cerevisiae) 4, Minichromosome maintenance deficient 4, Minichromosome maintenance deficient 4 homolog (S. cerevisiae), Minichromosome maintenance, S. cerevisiae, homolog of, 4, NKCD, NKGCD, P1-CDC21, hCdc 21, mKIAA4003, mcdc21

2 Images
Western blot - MCM4 overexpression 293T lysate (whole cell) (AB94302)
  • WB

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Western blot - MCM4 overexpression 293T lysate (whole cell) (AB94302)

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SDS-PAGE - MCM4 overexpression 293T lysate (whole cell) (AB94302)
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SDS-PAGE - MCM4 overexpression 293T lysate (whole cell) (AB94302)

ab94302 at 15μg/lane on an SDS-PAGE gel

Key facts

Species or organism

Human

Form

Liquid

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab94302 is a 293T cell transfected lysate in which Human MCM4 has been transiently over-expressed using a pCMV-MCM4 plasmid. The lysate is provided in 1X Sample Buffer.
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCM4 or Minichromosome Maintenance Complex Component 4 is a part of the MCM2-7 complex which is essential for the initiation and progression of DNA replication. It has a molecular mass of approximately 95 kDa. MCM4 functions as a DNA helicase unwinding DNA strands to allow replication forks to progress. This protein is expressed in various tissues with higher levels detected in dividing cells indicating its role in cell proliferation.
Biological function summary

MCM4 is a critical player in cell cycle regulation as it participates in the formation of the pre-replicative complex during the G1 phase of the cell cycle. Its function becomes evident as it interacts within the MCM2-7 hexameric complex important for helicase activity and stability. This complex prevents DNA re-replication within a single cell cycle ensuring the maintenance of genomic integrity.

Pathways

Several cellular mechanisms engage MCM4 particularly the DNA replication and cell cycle pathways. MCM4 associates with proteins like CDC45 and GINS within the CMG complex facilitating the activation and elongation phases of DNA replication. This relationship places MCM4 at the heart of orchestrating proper DNA synthesis and mitotic entry.

Researchers have linked MCM4 abnormalities to cancer progression and genomic instability. Overexpression or mutations in MCM4 disrupts normal replication processes heightening the risk for various cancers including breast and ovarian cancer. It also demonstrates interaction with the tumor suppressor protein p53 suggesting its involvement in processes that underpin malignancies linked to failed checkpoint control.
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Cell culture

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Product protocols

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Complementary Products

Primary Antibodies

AB124836

Anti-MCM4 antibody [EPR5245]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-MCM4 antibody [EPR5245] (AB124836)

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