MCT1/Monocarboxylic acid transporter 1 overexpression 293T lysate (whole cell)

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCT1 also known as Monocarboxylate Transporter 1 or SLC16A1 is a protein that facilitates the transport of monocarboxylates such as lactate and pyruvate across cell membranes. The approximate molecular weight of MCT1 is 51 kDa. This transporter expresses in many tissues including muscle brain liver and kidney. Its widespread presence indicates a significant role in cellular metabolism and energy balance across different biological systems.

Biological function summary

MCT1 acts as an important transport mechanism for lactate and pyruvate driving the cellular uptake and release processes of these molecules. It partners with CD147 also known as Basigin to form a functional heteromeric complex. This binding allows the correct localization and stabilization of MCT1 in the plasma membrane ensuring effective transport activity. The presence of MCT1 in metabolically active tissues highlights its role in maintaining homeostasis in energy-rich environments.

Pathways

MCT1 plays a significant role in the glycolysis and gluconeogenesis pathways by mediating lactate transport which connects anaerobic and aerobic metabolic processes. Through these pathways MCT1 associates with key metabolic enzymes like lactate dehydrogenase (LDH) that regulate the conversion between lactate and pyruvate. This association provides a link between lactate production and consumption thereby controlling pH levels and preventing the buildup of metabolic intermediates that can disrupt cellular functions.

MCT1 is implicated in cancer and metabolic acidosis. In cancer elevated expression of MCT1 supports the Warburg effect a phenomenon where cancer cells rely on aerobic glycolysis leading to increased lactate production. MCT1 works alongside proteins such as CD147 influencing tumor growth and metastasis by promoting lactate export. In metabolic acidosis impaired MCT1 function can disrupt lactate clearance affecting tissue pH balance and contributes to conditions like lactic acidosis. Understanding MCT1's role in these diseases highlights the importance of targeting its function for therapeutic purposes.