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AB94051

MGMT overexpression 293T lysate (whole cell)

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MGMT overexpression 293T lysate (whole cell) suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
3 Images
Western blot - MGMT overexpression 293T lysate (whole cell) (AB94051)
  • WB

Unknown

Western blot - MGMT overexpression 293T lysate (whole cell) (AB94051)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 92 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID : 19366806, 16840724).

All lanes:

Western blot - Anti-Eph receptor B4/HTK antibody [EPR23222-24] (<a href='/en-us/products/primary-antibodies/eph-receptor-b4-htk-antibody-epr23222-24-ab254301'>ab254301</a>) at 1/1000 dilution

Lane 1:

GR-M (mouse Caucasian melanoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

4T1 (mouse mammary gland carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Predicted band size: 108 kDa

Observed band size: 120 kDa

false

Western blot - MGMT overexpression 293T lysate (whole cell) (AB94051)
  • WB

Unknown

Western blot - MGMT overexpression 293T lysate (whole cell) (AB94051)

false

SDS-PAGE - MGMT overexpression 293T lysate (whole cell) (AB94051)
  • SDS-PAGE

Unknown

SDS-PAGE - MGMT overexpression 293T lysate (whole cell) (AB94051)

ab94051 at 15µg/lane on an SDS-PAGE gel

Key facts

Species or organism

Human

Form

Liquid

form

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Complementary Products

Primary Antibodies

AB39253

Anti-MGMT antibody [MT3.1]

BOND RX™ Validated|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGMT antibody [MT3.1] (AB39253)

  • 4
  • 6
Proteins & Peptides

AB79251

Recombinant Human MGMT protein

Western blot - Recombinant Human MGMT protein (AB79251)

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Assay Kits

AB320053

Human MGMT ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human MGMT ELISA Kit (AB320053)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab94051 is a 293T cell transfected lysate in which Human MGMT has been transiently over-expressed using a pCMV-MGMT plasmid. The lysate is provided in 1X Sample Buffer.
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MGMT also known as O-6-methylguanine-DNA methyltransferase is an enzyme with a significant role in DNA repair. MGMT protein has a molecular weight of about 22 kDa. This protein is typically expressed in various tissues including the liver kidney and brain where it functions to protect cells from mutagenic and cytotoxic alterations. MGMT works by transferring alkyl groups from the O6 position of guanine to a cysteine residue within its active site. This mechanism directly reverses DNA damage maintaining genomic stability.
Biological function summary

MGMT prevents mutations by reversing alkylation damage playing a significant role in preserving DNA integrity. It acts independently and does not form part of any larger protein complexes. This enzyme-mediated repair is central to cellular defense against mutagenic threats such as alkylating agents. By curbing DNA damage MGMT can thwart the onset of harmful genetic mutations that might lead to issues like cancer development.

Pathways

MGMT is intimately involved in DNA damage response and repair pathways. Notably it interacts with base excision repair and direct reversal repair pathways. MGMT's function is distinct but complementary to the activities of other DNA repair proteins like XRCC1 and APE1 which are involved in the base excision repair pathway. The presence of MGMT bolsters cellular resilience by ensuring that DNA lesions caused by alkylating agents are addressed promptly and effectively.

MGMT has a profound association with several types of cancer such as glioblastoma and colorectal cancer. Reduced MGMT expression or activity can lead to enhanced susceptibility to the carcinogenic effects of alkylating agents making cancer development more likely. Additionally MGMT inhibitors such as lomeguatrib are used therapeutically to enhance the effectiveness of alkylating chemotherapy. Within this context MGMT interacts with proteins involved in tumor suppression and cell cycle regulation including p53 influencing both cancer progression and treatment outcomes.
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Cell culture

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Product protocols

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Product promise

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