Mouse ARC knockout Neuro-2a cell lysate
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ARC KO cell lysate available now. KO validated. Free of charge wild type control included.
View Alternative Names
ARC/ARG3.1, ARC_HUMAN, Activity-regulated cytoskeleton-associated protein, Activity-regulated gene 3.1 protein homolog, Arg 3.1
- WB
Supplier Data
Western blot - Mouse ARC knockout Neuro-2a cell lysate (AB280130)
False colour image of Western blot : Anti-Arc antibody [EPR18950] staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab183183 was shown to bind specifically to Arc. A band was observed at 45 kDa in wild-type Neuro-2a cell lysates with no signal observed at this size in arc knockout cell line ab280071 (knockout cell lysate ab280130). To generate this image, wild-type and arc knockout Neuro-2a cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
false
- Sanger seq
Supplier Data
Sanger Sequencing - Mouse ARC knockout Neuro-2a cell lysate (AB280130)
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The Arc protein influences synaptic plasticity and memory formation. It does not operate alone; instead it interacts within complexes that regulate synaptic strength. These complexes contain other proteins critical for actin cytoskeleton remodeling which impacts synaptic efficacy. Through these interactions Arc supports the internalization of AMPA receptors playing a role in long-term depression (LTD) and long-term potentiation (LTP) of synapses essential mechanisms for learning and memory.
Pathways
The Arc protein forms integral parts of the synaptic signaling pathways. It connects with the BDNF-TrkB signaling pathway which is important for neuronal development and synaptic modulation. Arc also relates to the mTOR signaling pathway which governs cellular growth and protein synthesis. Through these pathways Arc interacts with proteins like Neuro2a and SynGAP highlighting its comprehensive role in synaptic regulation and plasticity.
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
![Western blot - Anti-Arc antibody [EPR18950] (AB183183)](https://content.abcam.com/products/images/arc-antibody-epr18950-ab183183--western-blot-img40716.jpg)
Product promise
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