Mouse CDKN1B (p27 KIP 1) knockout RAW 264.7 cell lysate
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(0 Publication)
- WB
Lab
Western blot - Mouse CDKN1B (p27 KIP 1) knockout RAW 264.7 cell lysate (AB282970)
Lane 1 : Wild-type RAW 264.7 cell lysate 20 μg
Lane 2 : CDKN1B knockout RAW 264.7 cell lysate 20 μg
False colour image of Western blot : Anti-p27 KIP 1 antibody [EPFHCR16] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab92741 was shown to bind specifically to p27 KIP 1. A band was observed at 28 kDa in wild-type RAW 264.7 cell lysates with no signal observed at this size in CDKN1B knockout cell line ab281619 (knockout cell lysate ab282970). To generate this image, wild-type and CDKN1B knockout RAW 264.7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-p27 KIP 1 antibody [EPFHCR16] (<a href='/en-us/products/primary-antibodies/p27-kip-1-antibody-epfhcr16-ab92741'>ab92741</a>) at 1/1000 dilution
Lane 1:
Wild-type RAW 264.7 cell lysate at 20 µg
Lane 2:
CDKN1B knockout RAW 264.7 cell lysate at 20 µg
Lane 2:
Western blot - Mouse CDKN1B (p27 KIP 1) knockout RAW 264.7 cell line (<a href='/en-us/products/cell-lines/mouse-cdkn1b-p27-kip-1-knockout-raw-2647-cell-line-ab281619'>ab281619</a>)
Predicted band size: 22 kDa
Observed band size: 28 kDa
false
- WB
Lab
Western blot - Mouse CDKN1B (p27 KIP 1) knockout RAW 264.7 cell lysate (AB282970)
Lane 1 : Wild-type RAW 264.7 cell lysate 20 μg
Lane 2 : CDKN1B knockout RAW 264.7 cell lysate 20 μg
False colour image of Western blot : Anti-p27 KIP 1 antibody [Y236] staining at 1/5000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab32034 was shown to bind specifically to p27 KIP 1. A band was observed at 28 kDa in wild-type RAW 264.7 cell lysates with no signal observed at this size in CDKN1B knockout cell line ab281619 (knockout cell lysate ab282970). To generate this image, wild-type and CDKN1B knockout RAW 264.7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-p27 KIP 1 antibody [Y236] (<a href='/en-us/products/primary-antibodies/p27-kip-1-antibody-y236-ab32034'>ab32034</a>) at 1/5000 dilution
Lane 1:
Wild-type RAW 264.7 cell lysate at 20 µg
Lane 2:
CDKN1B knockout RAW 264.7 cell lysate at 20 µg
Lane 2:
Western blot - Mouse CDKN1B (p27 KIP 1) knockout RAW 264.7 cell line (<a href='/en-us/products/cell-lines/mouse-cdkn1b-p27-kip-1-knockout-raw-2647-cell-line-ab281619'>ab281619</a>)
Predicted band size: 22 kDa
Observed band size: 28 kDa
false
- Sanger seq
Supplier Data
Sanger Sequencing - Mouse CDKN1B (p27 KIP 1) knockout RAW 264.7 cell lysate (AB282970)
52 bp deletion-3 bp insertion in Exon 1
Complementary Products
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [Y236] (AB32034)](https://content.abcam.com/products/images/p27-kip-1-antibody-y236-ab32034--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45173.jpg)
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The function of p27 KIP1 involves its role in controlling cell growth and division. It achieves this by becoming a part of larger protein complexes involving CDKs and cyclins. By directly interacting with these complexes p27 KIP1 modulates cell cycle progression therefore acting as a brake on cellular proliferation. The protein is important in maintaining proper cell cycle checkpoints and preventing uncontrolled cell growth which is essential for normal cellular functioning.
Pathways
The involvement of p27 KIP1 centers on cell cycle regulation and signaling pathways such as the PI3K/AKT pathway. Its interaction with CDKs and cyclins situates it within the core mechanisms that determine cell division timing. p27 KIP1 operates alongside other proteins like cyclin D and CDK4/6 fitting into the regulatory intricacies of these pathways. Proper functioning of these pathways ensures cellular homeostasis and prevents the development of oncogenic processes.
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Male
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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