JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB275524

Mouse CX3CR1 knockout RAW 264.7 cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

CX3CR1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 104 bp deletion in exon 2.

View Alternative Names

Beta chemokine receptor-like 1, C-X3-C CKR-1, CCRL1, CMK-BRL-1, CMKBLR1, CMKDR1, CX3C chemokine receptor 1, CX3C1_HUMAN, Chemokine C X3 C motif receptor 1, Fractalkine receptor, G-protein coupled receptor 13, GPR13, GPRV28, V28

1 Images
Sanger Sequencing - Mouse CX3CR1 knockout RAW 264.7 cell lysate (AB275524)
  • Sanger seq

Supplier Data

Sanger Sequencing - Mouse CX3CR1 knockout RAW 264.7 cell lysate (AB275524)

Allele 1 : 104 bp deletion in exon 2

Key facts

Cell type

RAW 264.7

Species or organism

Mouse

Tissue

Lymphatic

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 104 bp deletion in exon 2

Disease

Carcinoma

style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab275524-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Mouse wild-type RAW 264.7 cell lysate", "number":"AB275524-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Mouse CX3CR1 knockout RAW 264.7 cell lysate", "number":"AB275524-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
CX3CR1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CX3CR1 also known as fractalkine receptor is a seven-transmembrane G protein-coupled receptor. It has a molecular weight of about 40 kDa. This receptor is expressed in various tissues particularly on immune cells like monocytes natural killer cells T cells and microglia. CX3CR1 binds to its ligand CX3CL1 or fractalkine which is a chemokine involved in directing cell movement. The receptor-ligand interaction is critical for the adhesion and migration of leukocytes to inflammatory sites.
Biological function summary

CX3CR1 plays a significant role in immune regulation and cellular migration. It is not part of a larger protein complex but interacts directly with monocyte and microglia cells influencing their activity. CX3CR1 is recognized as a marker for microglia in flow cytometry applications assisting in the identification of these brain-resident immune cells. The receptor is frequently studied using monoclonal antibodies like anti-CX3CR1 and is subject to inhibition by CX3CR1 antagonists such as 8e10 affecting its function in immune responses.

Pathways

CX3CR1 involves itself heavily in the chemokine signaling pathway and the inflammatory response pathway. Its interaction with CX3CL1 influences the PI3K-Akt signaling pathway modulating cell survival and proliferation. The receptor works in conjunction with CCR2 in monocyte navigation impacting the inflammatory cascade during immune response. Their coordinated action is important for cellular trafficking to sites requiring immune intervention.

CX3CR1 has associations with neurodegenerative diseases such as Alzheimer's disease as well as with atherosclerosis. Changes in its expression and function can exacerbate these conditions. In Alzheimer's disease microglial CX3CR1 may mediate neuroinflammatory processes affecting plaque buildup. In atherosclerosis the receptor is involved in monocyte recruitment to plaques influenced by the related protein fractalkine. Understanding these relationships provides insights into potential therapeutic targets for modulating disease progression.
style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Male

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB245248

Anti-CX3CR1 antibody [EPR22354-15]

RabMAb|Recombinant|Trial Size|Trial Size

Flow Cytometry - Anti-CX3CR1 antibody [EPR22354-15] (AB245248)

  • 0
  • 0
Cell Lines & Lysates

AB273752

Mouse CX3CR1 knockout RAW 264.7 cell line

Sanger Sequencing - Mouse CX3CR1 knockout RAW 264.7 cell line (AB273752)

  • 0
  • 0
Primary Antibodies

AB209064

Anti-TMEM119 antibody [28-3] - Microglial marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (AB209064)

  • 4
  • 23
Primary Antibodies

AB25088

Anti-CX3CL1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CL1 antibody (AB25088)

  • 3
  • 6

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com