Raji whole cell lysate

4 Images

• WB

Project3376****

Western blot - Raji whole cell lysate (AB30124)

All lanes:

Western blot - Anti-BRD1/BRL antibody (<a href='/en-us/products/primary-antibodies/brd1-brl-antibody-ab71877'>ab71877</a>) at 1 µg/mL

Lane 1:

TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate

Lane 2:

Western blot - Raji whole cell lysate (ab30124) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Observed band size: 150 kDa

false

• WB

Project5519****

Western blot - Raji whole cell lysate (AB30124)

All lanes:

Western blot - Anti-SOCS2 antibody (<a href='/en-us/products/primary-antibodies/socs2-antibody-ab66733'>ab66733</a>) at 1 µg/mL

Lane 1:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

Western blot - SW480 whole cell lysate (<a href='/en-us/products/cell-lysates/sw480-whole-cell-lysate-ab3957'>ab3957</a>) at 10 µg

Lane 4:

Western blot - Raji whole cell lysate (ab30124) at 10 µg

Lane 5:

Western blot - Mouse thymus tissue lysate - total protein (<a href='/en-us/products/tissue-lysates/mouse-thymus-tissue-lysate-total-protein-ab29285'>ab29285</a>) at 10 µg

Lane 6:

MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

• WB

Project3500****

Western blot - Raji whole cell lysate (AB30124)

All lanes:

Western blot - Anti-ATG3 antibody (<a href='/en-us/products/primary-antibodies/atg3-antibody-ab66287'>ab66287</a>) at 1 µg/mL

Lane 1:

TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate at 10 µg

Lane 2:

Western blot - Raji whole cell lysate (ab30124) at 10 µg

Lane 3:

JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 35 kDa

Observed band size: 36 kDa,72 kDa

false

• WB

Project5690****

Western blot - Raji whole cell lysate (AB30124)

All lanes:

Western blot - Anti-HYAL2 antibody (<a href='/en-us/products/primary-antibodies/hyal2-antibody-ab68608'>ab68608</a>) at 1 µg/mL

Lane 1:

Liver (Rat) Tissue Lysate at 10 µg

Lane 2:

Liver (Mouse) Tissue Lysate at 10 µg

Lane 3:

JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Human thymus tissue lysate - total protein (<a href='/en-us/products/unavailable/human-thymus-tissue-lysate-total-protein-ab30146'>ab30146</a>) at 10 µg

Lane 5:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 6:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 7:

Western blot - Raji whole cell lysate (ab30124) at 10 µg

Lane 8:

Western blot - SW480 whole cell lysate (<a href='/en-us/products/cell-lysates/sw480-whole-cell-lysate-ab3957'>ab3957</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 54 kDa

Observed band size: 40 kDa,50 kDa,54 kDa,58 kDa

true