SNF2H overexpression 293T lysate (whole cell)

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNF2H also known as SMARCA5 is an ATP-dependent chromatin remodeler with a molecular mass of approximately 122 kDa. This protein belongs to the SNF2 family and functions as a motor protein utilizing the energy from ATP hydrolysis to alter chromatin structure. SNF2H expresses widely across multiple tissues with higher expression levels in the brain and reproductive organs. Its mechanical role involves sliding or repositioning nucleosomes along DNA which influences accessibility for transcription replication and repair machinery.

Biological function summary

In coordinating chromatin dynamics and nucleosome architecture SNF2H plays an important part in the ISWI (Imitation Switch) chromatin remodeling complex. This complex modulates chromatin organization to facilitate histone modification and DNA accessibility impacting gene expression regulation. SNF2H directly affects DNA replication and repair processes contributing to the maintenance of genomic stability. The structural flexibility provided by SNF2H is essential for cellular processes where timely and regulated DNA access is necessary.

Pathways

Modulation of chromatin architecture by SNF2H is key to both transcriptional regulation and DNA repair pathways. It participates in the DNA damage response pathway facilitating recruitment and assembly of repair proteins at damage sites. SNF2H also involves itself in transcriptional control through associations with transcription factors and other chromatin contractors such as NURF (Nucleosome Remodeling Factor) complex components which include proteins like BPTF. These interactions permit precise control of gene activation or repression in response to cellular signals.

Dysfunction of SNF2H impacts chromatin remodeling fidelity contributing to conditions such as cancer and developmental anomalies. Aberrations in SNF2H expression or activity can lead to genomic instability influencing cancer progression through altered transcriptional programs and impaired DNA repair mechanisms. Additionally SNF2H links to ATRX syndrome a developmental disorder associated with mutations in the related ATRX protein highlighting its involvement in maintaining chromatin integrity essential for normal development.