T-47D whole cell lysate

6 Images

• WB

Ap

Western blot - T-47D whole cell lysate (AB14899)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab12148 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-YB1 antibody (<a href='/en-us/products/primary-antibodies/yb1-antibody-ab12148'>ab12148</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

Jurkat (Human) Whole Cell Lysate at 10 µg

Lane 4:

Western blot - T-47D whole cell lysate (ab14899) at 10 µg

Lane 5:

MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 36 kDa

Observed band size: 100 kDa,50 kDa

true

Exposure time: 4min

• WB

Unknown

Western blot - T-47D whole cell lysate (AB14899)

We hypothesize that the 26 kDa band represents the cleaved form of MUC1. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Western blot - Anti-MUC1 antibody (<a href='/en-us/products/primary-antibodies/muc1-antibody-ab84597'>ab84597</a>) at 1 µg/mL

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

Lung (Human) Tissue Lysate at 10 µg

Lane 4:

Western blot - T-47D whole cell lysate (ab14899) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 122 kDa

Observed band size: 24 kDa,26 kDa

true

Exposure time: 2min

• WB

Ap19106****

Western blot - T-47D whole cell lysate (AB14899)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab43606 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-SGK1 antibody (<a href='/en-us/products/primary-antibodies/sgk1-antibody-ab43606'>ab43606</a>) at 1 µg/mL

Lane 1:

Human BT549 (Breast carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Western blot - T-47D whole cell lysate (ab14899) at 10 µg

Lane 3:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

Predicted band size: 48 kDa

Observed band size: 49 kDa

true

Exposure time: 1min

• WB

Unknown

Western blot - T-47D whole cell lysate (AB14899)

Secondary antibody - anti-rabbit HRP (ab6721)

All lanes:

Western blot - Anti-Hsp60 antibody - Mitochondrial Marker (<a href='/en-us/products/primary-antibodies/hsp60-antibody-mitochondrial-marker-ab46798'>ab46798</a>) at 1/50000 dilution

All lanes:

Western blot - T-47D whole cell lysate (ab14899) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) at 1/2000 dilution

Predicted band size: 61 kDa

Observed band size: 60 kDa

false

• WB

Unknown

Western blot - T-47D whole cell lysate (AB14899)

The 127 and 37 kDa bands correlate to DDB1 and GAPDH, respectively.

All lanes:

Western blot - Anti-DDB1 with anti-GAPDH internal loading control antibody (<a href='/en-us/products/primary-antibodies/ddb1-with-gapdh-internal-loading-control-antibody-ab75393'>ab75393</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Hela Whole Cell Lysate - UV Irradiated at 10 µg

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Western blot - T-47D whole cell lysate (ab14899) at 10 µg

Lane 5:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 6:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 7:

Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 127 kDa

Observed band size: 127 kDa,32 kDa,37 kDa,60 kDa

true

Exposure time: 30s

• WB

Unknown

Western blot - T-47D whole cell lysate (AB14899)

Wnt9a contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125957 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-Wnt9a antibody (<a href='/en-us/products/primary-antibodies/wnt9a-antibody-ab125957'>ab125957</a>) at 1 µg/mL

Lane 1:

Western blot - Mouse lung normal tissue lysate - total protein (<a href='/en-us/products/tissue-lysates/mouse-lung-normal-tissue-lysate-total-protein-ab29297'>ab29297</a>) at 10 µg

Lane 2:

Heart (Mouse) Tissue Lysate at 10 µg

Lane 3:

Western blot - T-47D whole cell lysate (ab14899) at 10 µg

Lane 4:

PANC-1 (Human Pancreatic Carcinoma) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 40 kDa

Observed band size: 110 kDa,43 kDa,70 kDa

true

Exposure time: 1min