T-47D whole cell lysate

6 product images

• 

  Western blot - T-47D whole cell lysate (ab14899)

  The 127 and 37 kDa bands correlate to DDB1 and GAPDH, respectively.

  All lanes: Western blot - Anti-DDB1 with anti-GAPDH internal loading control antibody (Anti-DDB1 with anti-GAPDH internal loading control antibody ab75393) at 1 µg/mL

  Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 2: Hela Whole Cell Lysate - UV Irradiated at 10 µg

  Lane 3: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 4: Western blot - T-47D whole cell lysate (ab14899) at 10 µg

  Lane 5: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 6: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

  Lane 7: Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 127 kDa

  Observed band size: 127 kDa, 32 kDa, 37 kDa, 60 kDa

  Exposure time: 30s

• 

  Western blot - T-47D whole cell lysate (ab14899)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with Anti-YB1 antibody ab12148 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - Anti-YB1 antibody (Anti-YB1 antibody ab12148) at 1 µg/mL

  Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 2: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 3: Jurkat (Human) Whole Cell Lysate at 10 µg

  Lane 4: Western blot - T-47D whole cell lysate (ab14899) at 10 µg

  Lane 5: MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

  Secondary

  All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 36 kDa

  Observed band size: 100 kDa, 50 kDa

  Exposure time: 4min

• 

  Western blot - T-47D whole cell lysate (ab14899)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with Anti-SGK1 antibody ab43606 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - Anti-SGK1 antibody (Anti-SGK1 antibody ab43606) at 1 µg/mL

  Lane 1: Human BT549 (Breast carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 2: Western blot - T-47D whole cell lysate (ab14899) at 10 µg

  Lane 3: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 48 kDa

  Observed band size: 49 kDa

  Exposure time: 1min

• 

  Western blot - T-47D whole cell lysate (ab14899)

  Wnt9a contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with Anti-Wnt9a antibody ab125957 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  All lanes: Western blot - Anti-Wnt9a antibody (Anti-Wnt9a antibody ab125957) at 1 µg/mL

  Lane 1: Western blot - Mouse lung normal tissue lysate - total protein (Mouse lung normal tissue lysate - total protein ab29297) at 10 µg

  Lane 2: Heart (Mouse) Tissue Lysate at 10 µg

  Lane 3: Western blot - T-47D whole cell lysate (ab14899) at 10 µg

  Lane 4: PANC-1 (Human Pancreatic Carcinoma) Whole Cell Lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 40 kDa

  Observed band size: 110 kDa, 43 kDa, 70 kDa

  Exposure time: 1min

• 

  Western blot - T-47D whole cell lysate (ab14899)

  We hypothesize that the 26 kDa band represents the cleaved form of MUC1. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  All lanes: Western blot - Anti-MUC1 antibody (Anti-MUC1 antibody ab84597) at 1 µg/mL

  Lane 1: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 2: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 3: Lung (Human) Tissue Lysate at 10 µg

  Lane 4: Western blot - T-47D whole cell lysate (ab14899) at 10 µg

  Secondary

  All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 122 kDa

  Observed band size: 24 kDa, 26 kDa

  Exposure time: 2min

• 

  Western blot - T-47D whole cell lysate (ab14899)

  Secondary antibody - anti-rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab6721)

  All lanes: Western blot - Anti-Hsp60 antibody (Anti-Hsp60 antibody ab46798) at 1/50000 dilution

  All lanes: Western blot - T-47D whole cell lysate (ab14899) at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/2000 dilution

  Predicted band size: 61 kDa

  Observed band size: 60 kDa