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AB94136

XPD overexpression 293T lysate (whole cell)

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XPD overexpression 293T lysate (whole cell) suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

View Alternative Names

BTF2 p80, Basic transcription factor 2 80 kDa subunit, COFS 2, CXPD, DNA excision repair protein ERCC-2, DNA repair protein complementing XP-D cells, EM9, ERCC2_HUMAN, Excision repair 2, Excision repair cross complementing rodent repair deficiency complementation, Excision repair cross complementing rodent repair deficiency, complementation group 2, MGC102762, MGC126218, MGC126219, OTTHUMP00000045860, OTTHUMP00000045861, OTTHUMP00000045862, OTTHUMP00000045863, TFIIH 80 kDa subunit, TFIIH Basal Transcription Factor Complex Helicase Subunit, TFIIH basal transcription factor complex 80 kDa subunit, TFIIH basal transcription factor complex helicase XPD subunit, TFIIH basal transcription factor complex p80 subunit, TFIIH p80, XPDC, Xeroderma pigmentosum complementary group D, Xeroderma pigmentosum group D-complementing protein

2 Images
Western blot - XPD overexpression 293T lysate (whole cell) (AB94136)
  • WB

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Western blot - XPD overexpression 293T lysate (whole cell) (AB94136)

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SDS-PAGE - XPD overexpression 293T lysate (whole cell) (AB94136)
  • SDS-PAGE

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SDS-PAGE - XPD overexpression 293T lysate (whole cell) (AB94136)

ab94136 at 15µg/lane on an SDS-PAGE gel.

Key facts

Cell type

HEK-293T

Species or organism

Human

Form

Liquid

form

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Complementary Products

Primary Antibodies

AB238930

Anti-XPD antibody [EPR9675] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPD antibody [EPR9675] - BSA and Azide free (AB238930)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab94136 is a 293T cell transfected lysate in which Human XPD has been transiently over-expressed using a pCMV-XPD plasmid. The lysate is provided in 1X Sample Buffer.
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

XPD also known as ERCC2 is an essential helicase enzyme belonging to the family of ATP-dependent DNA helicases. It consists of approximately 761 amino acids and has a mass of around 87 kDa. XPD functions mechanically by unwinding double-stranded DNA which is important for various DNA repair processes. This protein is expressed ubiquitously in human tissues highlighting its critical role in maintaining genomic integrity.
Biological function summary

XPD catalyzes the unwinding of DNA within the transcription factor IIH (TFIIH) complex. This complex facilitates DNA repair and transcription initiation. XPD participates in the nucleotide excision repair (NER) pathway where it contributes to repairing damaged DNA by excising damaged nucleotides. Its role in transcription-coupled repair and general transcription initiation underlines its importance in cellular homeostasis.

Pathways

DNA repair and general transcription processes are significantly affected by XPD. It plays an integral role in nucleotide excision repair (NER) and transcription. Within these pathways XPD works closely with other proteins such as XPA which assists in damage verification and incision near DNA lesions. XPD's unwinding function is essential for the correct orientation of DNA during transcription and repair events ensuring accurate gene expression and stability.

XPD mutations link to xeroderma pigmentosum (XP) and trichothiodystrophy (TTD). These conditions often result in increased sensitivity to ultraviolet (UV) radiation and various developmental abnormalities. XPD mutations impair the repair of UV-induced DNA damage causing symptoms in both XP and TTD. XPD also connects with ERCC1 a protein important for repair synthesis and DNA incision highlighting its broad impact on human health when its function is compromised.
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Cell culture

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Product protocols

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Product promise

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