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ChIP kit ab500 provides a protocol and reagents for running ChIP assays including:
- cell lysis and chromatin extraction
- chromatin shearing and DNA fragment length analysis
- immunoprecipitation and DNA purification
DNA produced using the kit can be analyzed using qPCR.
- Sample type
Suspension cells, Adherent cells
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ChIP-seq | Reactivity No | Dilution info - | Notes - |
Application ChIP | Reactivity Reacts | Dilution info - | Notes - |
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ChIP kit ab500 provides a protocol and reagents for running ChIP assays including:
- cell lysis and chromatin extraction
- chromatin shearing and DNA fragment length analysis
- immunoprecipitation and DNA purification
DNA produced using the kit can be analyzed using qPCR.
Key facts
- Sample type
Suspension cells, Adherent cells
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
Multi
- Appropriate long-term storage conditions
Multi
- Storage information
Please refer to protocols
Notes
ChIP kit ab500 provides a protocol and reagents for running ChIP assays including:
- cell lysis and chromatin extraction
- chromatin shearing and DNA fragment length analysis
- immunoprecipitation and DNA purification
DNA produced using the kit can be analyzed using qPCR.
The kit has been validated for ChIP assays with mammalian samples.
Chinese protocol available in the support and downloads section.
This kit uses Protein A sepharose beads for antibody pulldown. See table below for Protein A and Protein G binding affinities with antibodies from commonly used species. For other species of antibody, consult the table in the protocol booklet. **ChIP assay products and guides** Find more resources and products, products, and other .
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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