Skip to main content

ChIP kit ab500 provides a protocol and reagents for running ChIP assays including:
- cell lysis and chromatin extraction
- chromatin shearing and DNA fragment length analysis
- immunoprecipitation and DNA purification

DNA produced using the kit can be analyzed using qPCR.

Sample type

Suspension cells, Adherent cells

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Application
ChIP-seq
Reactivity
No
Dilution info
-
Notes

-

Application
ChIP
Reactivity
Reacts
Dilution info
-
Notes

-

Images

What's included?

1 Kit
Components
1.25M Glycine
1 x 10 mL
Buffer A
1 x 10 mL
Buffer B
1 x 30 mL
Buffer C
1 x 30 mL
Buffer D
1 x 3 mL
ChIP Buffer 5X
1 x 84 mL
DNA Purifying Slurry
1 x 4 mL
PCR-Grade Water
1 x 10.2 mL
Protease Inhibitors
1 x 1 Tablet
Protein A Beads Unblocked
1 x 960 µL
Proteinase K
1 x 40 µL

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

ChIP kit ab500 provides a protocol and reagents for running ChIP assays including:
- cell lysis and chromatin extraction
- chromatin shearing and DNA fragment length analysis
- immunoprecipitation and DNA purification

DNA produced using the kit can be analyzed using qPCR.

Key facts

Sample type

Suspension cells, Adherent cells

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

ChIP kit ab500 provides a protocol and reagents for running ChIP assays including:
- cell lysis and chromatin extraction
- chromatin shearing and DNA fragment length analysis
- immunoprecipitation and DNA purification

DNA produced using the kit can be analyzed using qPCR.

The kit has been validated for ChIP assays with mammalian samples.

Chinese protocol available in the support and downloads section.

This kit uses Protein A sepharose beads for antibody pulldown. See table below for Protein A and Protein G binding affinities with antibodies from commonly used species. For other species of antibody, consult the table in the protocol booklet. **ChIP assay products and guides** Find more resources and products, products, and other .

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com