ChIP Kit

5 product images

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  ChIP - ChIP Kit (ab500)

  Chromatin immunoprecipitation using ab500 ChIP Kit and Histone H3 antibody (Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade ab1791). Chromatin was prepared from HeLa cells using the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. ChIP was performed with 2 μg of Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade ab1791 (blue). No antibody was added to the beads control (yellow). Immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active/inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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  Li Q., PLoS One 8(9). Fig 2c. doi: 10.1371/journal.pone.0075470. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  ChIP - ChIP Kit (ab500)

  Chromatin immunoprecipitation assay was performed to define the interaction of serum response factor (SRF) with the intragenic serum response element (SRE) regulatory motif in mouse using ab500 ChIP Kit.

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  ChIP - ChIP Kit (ab500)

  Chromatin immunoprecipitation using ab500 ChIP Kit and Histone H3 (tri methyl K9) antibody (Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade ab8898). Chromatin was prepared from HeLa cells using the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. ChIP was performed with 2 μg of Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade ab8898 (blue). No antibody was added to the beads control (yellow). Immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active/inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of transcribed region.
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  Western blot - ChIP Kit (ab500)

  Lane 1: HL60 cell lysate

  Lane 2: HL29 cell lysate

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  ChIP - ChIP Kit (ab500)

  Chromatin immunoprecipitation using ab500 ChIP Kit and Histone H3 (tri methyl K4) antibody (Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade ab12209). Chromatin was prepared from HeLa cells using the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. ChIP was performed with 5 μg of Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade ab12209 (blue). No antibody was added to the beads control (yellow). Immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active/inactive loci). Primers and probes are located in the first kb of the transcribed region.