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ChIP Kit (FFPE samples) (ab270815) provides a unique and robust ChIP protocol suitable for investigation of proteins associated with chromatin from FFPE samples and provides high quality yields of DNA.

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What's included?

24 Test
Components
5% BSA (DNA free)
1 x 144 µL
5x ChIP Buffer C1b
1 x 6.8 mL
ChIP DNA Binding Buffer
1 x 50 mL
ChIP-seq grade water
1 x 26.6 mL
DNA Elution Buffer
1 x 10 mL
DNA Wash Buffer
1 x 6 mL
Elution Buffer E1
1 x 7.8 mL
Elution Buffer E2
1 x 320 µL
Filters
10 x 1 Unit
Lysis Buffer L1b
1 x 6 mL
Lysis Buffer L2
1 x 6 mL
Protease inhibitor cocktail
1 x 80 µL
Protein A-coated magnetic beads
1 x 480 µL
Shearing Buffer S1b
1 x 6 mL
Spin columns
50 x 1 Unit
Wash Buffer W4
1 x 8.4 mL
Wash buffer W1
1 x 8.4 mL
Wash buffer W2
1 x 8.4 mL
Wash buffer W3
1 x 8.4 mL

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ChIP Kit (FFPE samples) (ab270815) provides a unique and robust ChIP protocol suitable for investigation of proteins associated with chromatin from FFPE samples and provides high quality yields of DNA.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

ChIP Kit (FFPE samples) (ab270815) provides a unique and robust ChIP protocol suitable for investigation of proteins associated with chromatin from FFPE samples and provides high quality yields of DNA. This solution makes traditionally difficult work with FFPE samples simpler and safer. The protocol involves easy and quick and non-toxic deparaffinization, followed by chromatin extraction and shearing by sonication. The kit contains everything you need for start-to-finish chromatin immunoprecipitation with an optimized protocol to make ChIP from FFPE samples simple yet reliable. In addition, the kit has been thoroughly validated and optimized for histones and transcription factors.

This kit contains enough reagents to perform 10 individual chromatin preparation from FFPE sections, up to 24 immunoprecipitations and 50 DNA purifications (from 10 input, 24 IPs and 10 shearing assessment samples).

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com

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