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Abcam's ChIP Kit Magnetic - One Step (ab156907) is a complete set of optimized buffers and reagents to carry out a successful chromatin immunoprecipitation for the selective enrichment of a chromatin fraction containing specific DNA sequences from isolated chromatin, in order to investigate the interaction between proteins and DNA in a cell.

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ChIP - ChIP Kit Magnetic - One Step (AB156907), expandable thumbnail

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Key facts

Assay time
5h

Reactivity data

Application
ChIP
Reactivity
Reacts
Dilution info
-
Notes

The GAPDH primers provided with the kit are for the human sequence. If using the kit with a different species, GAPDH primers for that species will need to be acquired.

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What's included?

96 Test
Components
10X Wash Buffer
1 x 20 mL
8-Well Strip Caps
1 x 24 Unit
96-Well PCR Plate
1 x 2 Unit
Anti-RNA Polymerase II (1 mg/mL)
1 x 16 µL
ChIP Buffer
1 x 12 mL
DNA Release Buffer
1 x 28 mL
GAPDH Primer - Forward (20 µM)
1 x 16 µL
GAPDH Primer - Reverse (20 µM)
1 x 16 µL
Magnetic Beads
1 x 450 µL
Non-Immune IgG (1 mg/mL)
1 x 20 µL
Proteinase K (10 mg/mL)
1 x 110 µL

Recommended products

Abcam's ChIP Kit Magnetic - One Step (ab156907) is a complete set of optimized buffers and reagents to carry out a successful chromatin immunoprecipitation for the selective enrichment of a chromatin fraction containing specific DNA sequences from isolated chromatin, in order to investigate the interaction between proteins and DNA in a cell.

Key facts

Assay time
5h

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

Abcam's ChIP Kit Magnetic - One Step (ab156907) is a complete set of optimized buffers and reagents to carry out a successful chromatin immunoprecipitation for the selective enrichment of a chromatin fraction containing specific DNA sequences from isolated chromatin, in order to investigate the interaction between proteins and DNA in a cell. For best result, we recommend the use of Chromatin Extraction Kit (Chromatin Extraction Kit ab117152) for sample preparation.

Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In mammalian cells, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.

**Primers**The GAPDH primers provided with the kit are for the human sequence. If using the kit with a difference species, GAPDH primers for that species will need to be acquired.**ChIP assay products and guides**Find more resources and products, products, and other .

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • ChIP - ChIP Kit Magnetic - One Step (ab156907), expandable thumbnail

    ChIP - ChIP Kit Magnetic - One Step (ab156907)

    Typical Results.

    The data shows the analysis of enrichment of RNA polymerase II in GAPDH and MLH1 promoters by Abcam's ChIP Kit Magnetic - One Step (ab156907) with chromatin extract prepared from formaldehyde fixed colon cancer cells. Captured DNA was used for analyzing levels of RNA polymerase II enriched in the GAPDH and MLH1 promoters.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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