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AB156907

ChIP Kit Magnetic - One Step

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(22 Publications)

Abcam's ChIP Kit Magnetic - One Step (ab156907) is a complete set of optimized buffers and reagents to carry out a successful chromatin immunoprecipitation for the selective enrichment of a chromatin fraction containing specific DNA sequences from isolated chromatin, in order to investigate the interaction between proteins and DNA in a cell.
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ChIP - ChIP Kit Magnetic - One Step (AB156907)
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Supplier Data

ChIP - ChIP Kit Magnetic - One Step (AB156907)

Typical Results.

The data shows the analysis of enrichment of RNA polymerase II in GAPDH and MLH1 promoters by Abcam's ChIP Kit Magnetic - One Step (ab156907) with chromatin extract prepared from formaldehyde fixed colon cancer cells. Captured DNA was used for analyzing levels of RNA polymerase II enriched in the GAPDH and MLH1 promoters.

Key facts

Assay time

5h

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ChIP": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>The GAPDH primers provided with the kit are for the human sequence. If using the kit with a different species, GAPDH primers for that species will need to be acquired.</p>" } } }
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Product details

Abcam's ChIP Kit Magnetic - One Step (ab156907) is a complete set of optimized buffers and reagents to carry out a successful chromatin immunoprecipitation for the selective enrichment of a chromatin fraction containing specific DNA sequences from isolated chromatin, in order to investigate the interaction between proteins and DNA in a cell. For best result, we recommend the use of Chromatin Extraction Kit (ab117152) for sample preparation.

Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In mammalian cells, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.

**Primers**The GAPDH primers provided with the kit are for the human sequence. If using the kit with a difference species, GAPDH primers for that species will need to be acquired.**ChIP assay products and guides**Find more resources and products, products, and other .

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & therapy 25:2329372 PubMed38494680

2024

CPT1A mediates the succinylation of SP5 which activates transcription of PDPK1 to promote the viability and glycolysis of prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shufeng Liu,Xiaoguang Chen,Liqi Zhang,Bo Lu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2302368 PubMed37949681

2023

Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Huaiyuan Liang,Chunguang Yang,Ruijiang Zeng,Yingqiu Song,Jianxi Wang,Wei Xiong,Binyuan Yan,Xin Jin

Journal of orthopaedic surgery and research 18:637 PubMed37644500

2023

FOXO1 regulates osteogenic differentiation of periodontal ligament stem cells through the METTL3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Wang,Wei Shi,Shaozhan Lin,Hanxue Wang

Journal of cellular physiology 238:992-1005 PubMed36852589

2023

Obesity-mediated upregulation of the YAP/IL33 signaling axis promotes aggressiveness and induces an immunosuppressive tumor microenvironment in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Zih Dai,Ching-Chieh Yang,Pei-Wei Shueng,Yen-Ju Wang,Chen-Shiuan Huang,Yi-Chun Chao,Cheng-Hsun Chen,Cheng-Wei Lin

Journal of nanobiotechnology 21:37 PubMed36732759

2023

Apoptotic tumor cell-derived microparticles loading Napabucasin inhibit CSCs and synergistic immune therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Boping Jing,Feng Guo,Rui An,Yu Gao,Yuman Li,Yuji Xie,Jing Wang,Yihan Chen,He Li,Tang Gao,Qiaofeng Jin,Li Zhang,Mingxing Xie

Cell death & disease 14:47 PubMed36670097

2023

TRIM15 forms a regulatory loop with the AKT/FOXO1 axis and LASP1 to modulate the sensitivity of HCC cells to TKIs.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Yang,Xin Jin,Xingchao Liu,Gang Wu,Wenhao Yang,Beichuan Pang,Jipeng Jiang,Dongxu Liao,Yu Zhang

British journal of cancer 128:232-244 PubMed36396821

2022

Downregulation of CBX7 induced by EZH2 upregulates FGFR3 expression to reduce sensitivity to cisplatin in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiannan Ren,Haixin Yu,Wei Li,Xin Jin,Bin Yan

Journal of experimental & clinical cancer research : CR 41:250 PubMed35974388

2022

YTHDC1 is downregulated by the YY1/HDAC2 complex and controls the sensitivity of ccRCC to sunitinib by targeting the ANXA1-MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Kun Ye,Xurui Li,Xinlin Liu,Mou Peng,Fang Chen,Wei Xiong,Yinhuai Wang,Liang Zhu

International journal of biological sciences 18:2132-2145 PubMed35342353

2022

The RNF26/CBX7 axis modulates the TNF pathway to promote cell proliferation and regulate sensitivity to TKIs in ccRCC.

Applications

Unspecified application

Species

Unspecified reactive species

Wentao Liu,Haohui Wang,Chengzhu Jian,Wei Li,Kun Ye,Jiannan Ren,Liang Zhu,Yinhuai Wang,Xin Jin,Lu Yi

Oncogene 41:538-549 PubMed34785774

2021

Smoking-associated upregulation of CBX3 suppresses ARHGAP24 expression to activate Rac1 signaling and promote tumor progression in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Jin,Bin Zhang,Hao Zhang,Haixin Yu
View all publications
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