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Abcam's ChIP Kit Magnetic - One Step (ab156907) is a complete set of optimized buffers and reagents to carry out a successful chromatin immunoprecipitation for the selective enrichment of a chromatin fraction containing specific DNA sequences from isolated chromatin, in order to investigate the interaction between proteins and DNA in a cell.

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Assay time

5h

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Reactivity data

Application
ChIP
Reactivity
Reacts
Dilution info
-
Notes

The GAPDH primers provided with the kit are for the human sequence. If using the kit with a different species, GAPDH primers for that species will need to be acquired.

Images

What's included?

96 Test
Components
10X Wash Buffer
1 x 20 mL
8-Well Strip Caps
1 x 24 Unit
96-Well PCR Plate
1 x 2 Unit
Anti-RNA Polymerase II (1 mg/mL)
1 x 16 µL
ChIP Buffer
1 x 12 mL
DNA Release Buffer
1 x 28 mL
GAPDH Primer - Forward (20 µM)
1 x 16 µL
GAPDH Primer - Reverse (20 µM)
1 x 16 µL
Magnetic Beads
1 x 450 µL
Non-Immune IgG (1 mg/mL)
1 x 20 µL
Proteinase K (10 mg/mL)
1 x 110 µL

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Abcam's ChIP Kit Magnetic - One Step (ab156907) is a complete set of optimized buffers and reagents to carry out a successful chromatin immunoprecipitation for the selective enrichment of a chromatin fraction containing specific DNA sequences from isolated chromatin, in order to investigate the interaction between proteins and DNA in a cell.

Key facts

Assay time

5h

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Abcam's ChIP Kit Magnetic - One Step (ab156907) is a complete set of optimized buffers and reagents to carry out a successful chromatin immunoprecipitation for the selective enrichment of a chromatin fraction containing specific DNA sequences from isolated chromatin, in order to investigate the interaction between proteins and DNA in a cell. For best result, we recommend the use of Chromatin Extraction Kit (ab117152) for sample preparation.

Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In mammalian cells, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.

**Primers**The GAPDH primers provided with the kit are for the human sequence. If using the kit with a difference species, GAPDH primers for that species will need to be acquired.**ChIP assay products and guides**Find more resources and products, products, and other .

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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