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AB270816

ChIP Kit (Magnetic, qPCR)

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(4 Publications)

ChIP Kit (Magnetic, qPCR) (ab270816) is optimized and validated for chromatin preparation and immunoprecipitation from cells and tissues for histones and transcriptional factors for ChIP-qPCR.

-Includes all buffers and reagents for chromatin shearing, immunoprecipitation, and DNA isolation
- Protein A magnetic beads are used to isolate the protein-DNA complexes of interest
- Eluted DNA suitable for qPCR analysis

Key facts

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Product details

ChIP Kit (Magnetic, qPCR) (ab270816) is a highly optimized and validated solution for ChIP-qPCR assays for either histones or transcription factors. The complete kit contains everything you need for start-to-finish ChIP including all validated buffers and reagents for chromatin shearing, immunoprecipitation, and DNA isolation for exceptional ChIP-qPCR results.

This kit uses a unique and fast method for DNA isolation and decrosslinking within 30 minutes compared to the standard 4 hours. Overall, easy to use and rapid protocol allows to receive the results within 20 hours with 4 hours hands-on time.

This kit contains enough reagents to perform 4 chromatin preparations, 24 chromatin immunoprecipitations and 32 DNA isolations.

Sample size:

Histones -

Cells (100,000 cells to 1,000,000 cells per IP)

Tissues (1.5 mg to 5 mg of tissue per IP)

Transciption factors -

Cells (4,000,000 cells per IP)

Tissues (30 mg of tissue per IP)

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 9:392 PubMed37875480

2023

Nucleophosmin 1 cooperates with BRD4 to facilitate c-Myc transcription to promote prostate cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Hong,Chengdang Xu,Shengfeng Zheng,Xinan Wang,Yiran Tao,Yao Tan,Guowen Lin,Denglong Wu,Dingwei Ye

Cells 12: PubMed37443715

2023

Allele-Specific Epigenetic Regulation of Expression at a Coronary Artery Disease Susceptibility Locus.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yang,Junjun Cao,David G McVey,Shu Ye

Nucleic acids research 51:5271-5284 PubMed37094074

2023

Phase-separated DropCRISPRa platform for efficient gene activation in mammalian cells and mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shufeng Ma,Kaitong Liao,Mengrao Li,Xinlong Wang,Jie Lv,Xin Zhang,Hongxin Huang,Lian Li,Tao Huang,Xiaohua Guo,Ying Lin,Zhili Rong

Clinical and translational medicine 11:e495 PubMed34323404

2021

Histone acetyltransferase 1 upregulates androgen receptor expression to modulate CRPC cell resistance to enzalutamide.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Hong,Zhendong Xiang,Pan Zhang,Qiang Wu,Chengdang Xu,Xinan Wang,Guowei Shi,Zongyuan Hong,Denglong Wu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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