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AB117138

ChIP Kit - One Step

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(22 Publications)

Abcam's ChIP Kit - One Step (ab117138) enables the user to enrich a chromatin fraction containing specific DNA sequences from isolated chromatin to be able to investigate the interaction between proteins and DNA.
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ChIP - ChIP Kit - One Step (AB117138)
  • ChIP

Project

ChIP - ChIP Kit - One Step (AB117138)

Chromatin was prepared from HeLa cells following the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. ChIP was performed with ab8580 (Histone H3 tri-methyl K4)using ab117138. No antibody was added to the blank (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primes and probes are located in the first Kb of the transcribed region.

ChIP - ChIP Kit - One Step (AB117138)
  • ChIP

Supplier Data

ChIP - ChIP Kit - One Step (AB117138)

The analysis of enrichment of RNA polymerase II in GAPDH and MLH1 promoters by Abcam's ChIP Kit - One Step, with chromatin extract prepared from formaldehyde fixed colon cancer cells. Captured DNA was used for analyzing levels of RNA polymerase II enriched in the GAPDH and MLH1 promoters.

Key facts

Assay time

5h

Assay type

Quantitative

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ChIP": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>The GAPDH primers provided with the kit are for the human sequence. If using the kit with a different species, GAPDH primers for that species will need to be acquired.</p>" } } }
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Product details

Abcam's ChIP Kit - One Step (ab117138) enables the user to enrich a chromatin fraction containing specific DNA sequences from isolated chromatin to be able to investigate the interaction between proteins and DNA. For best result, we recommend the use of Chromatin Extraction Kit (ab117152) for sample preparation.

Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In mammalian cells, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.

**Primers** The GAPDH primers provided with the kit are for the human sequence. If using the kit with a difference species, GAPDH primers for that species will need to be acquired.  **ChIP assay products and guides** Find more resources and products, products, and other .

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 21:126 PubMed37268943

2023

LOX-1 mediates inflammatory activation of microglial cells through the p38-MAPK/NF-κB pathways under hypoxic-ischemic conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshinori Aoki,Hongmei Dai,Fumika Furuta,Tomohisa Akamatsu,Takuya Oshima,Naoto Takahashi,Yu-Ichi Goto,Akira Oka,Masayuki Itoh

eLife 12: PubMed37261432

2023

Identification of Paired-related Homeobox Protein 1 as a key mesenchymal transcription factor in pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Emmeline Marchal-Duval,Méline Homps-Legrand,Antoine Froidure,Madeleine Jaillet,Mada Ghanem,Deneuville Lou,Aurélien Justet,Arnaud Maurac,Aurelie Vadel,Emilie Fortas,Aurelie Cazes,Audrey Joannes,Laura Giersh,Herve Mal,Pierre Mordant,Tristan Piolot,Marin Truchin,Carine M Mounier,Ksenija Schirduan,Martina Korfei,Andreas Gunther,Bernard Mari,Frank Jaschinski,Bruno Crestani,Arnaud A Mailleux

Birth defects research 115:770-781 PubMed36899481

2023

Effects of ketamine-induced H3K9 hypoacetylation during pregnancy on cardiogenesis of mouse offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Yujuan Yu,Junjun Quan,Mou Zou,Wei Zhao,Yujuan Su,Ying Xu

Cancer science 114:2053-2062 PubMed36308276

2023

Feedforward loop between IMP1 and YAP/TAZ promotes tumorigenesis and malignant progression in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Yang,Xujia Wu,Jia Wang,Xing Guo,Junju Chen,Xuesong Yang,Jian Zhong,Xixi Li,Zhong Deng

Cell & bioscience 12:135 PubMed35989326

2022

Anti-tumor effects of dual PI3K-HDAC inhibitor CUDC-907 on activation of ROS-IRE1α-JNK-mediated cytotoxic autophagy in esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Jian,Yichao Han,Wentian Zhang,Chengqiang Li,Wei Guo,Xijia Feng,Bin Li,Hecheng Li

Nature communications 13:4795 PubMed35970825

2022

Circular EZH2-encoded EZH2-92aa mediates immune evasion in glioblastoma via inhibition of surface NKG2D ligands.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Zhong,Xuesong Yang,Junju Chen,Kejun He,Xinya Gao,Xujia Wu,Maolei Zhang,Huangkai Zhou,Feizhe Xiao,Lele An,Xiuxing Wang,Yu Shi,Nu Zhang

Journal of animal science 100: PubMed35419600

2022

Identification of functional single nucleotide polymorphisms in the porcine SLC6A4 gene associated with aggressive behavior in weaned pigs after mixing.

Applications

Unspecified application

Species

Unspecified reactive species

Yanli Guo,Jing Zhao,Qinglei Xu,Siyuan Gao,Mingzheng Liu,Chunlei Zhang,Allan P Schinckel,Bo Zhou

Cell death discovery 8:107 PubMed35264565

2022

WWP1 upregulation predicts poor prognosis and promotes tumor progression by regulating ubiquitination of NDFIP1 in intrahepatic cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yongjian Li,Qian Cheng,Jie Gao,Zhuomiaoyu Chen,Jingheng Guo,Zuyin Li,Lingyu Tian,Chao Zhang,Yuzi Li,Jiaxi Zheng,Zhao Li,Jiye Zhu

Cell death discovery 8:103 PubMed35256601

2022

LncRNA MEG3 up-regulates SIRT6 by ubiquitinating EZH2 and alleviates nonalcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Zou,Liang Liu,Yinzhen Zeng,Huanhuan Wang,Dongling Dai,Mingguo Xu

Genetics in medicine : official journal of the American College of Medical Genetics 24:1085-1095 PubMed35168889

2022

Loss-of-function variants in KLF4 underlie autosomal dominant palmoplantar keratoderma.

Applications

Unspecified application

Species

Unspecified reactive species

Kiril Malovitski,Ofer Sarig,Sari Assaf,Janan Mohamad,Liron Malki,Shir Bergson,Alon Peled,Marina Eskin-Schwartz,Andrea Gat,Mor Pavlovsky,Eli Sprecher
View all publications
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