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Abcam's ChIP Kit - One Step (ab117138) enables the user to enrich a chromatin fraction containing specific DNA sequences from isolated chromatin to be able to investigate the interaction between proteins and DNA.

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Assay time

5h

Assay type

Quantitative

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Reactivity data

Application
ChIP
Reactivity
Reacts
Dilution info
-
Notes

The GAPDH primers provided with the kit are for the human sequence. If using the kit with a different species, GAPDH primers for that species will need to be acquired.

Images

What's included?

96 Test
Components
10X Wash Buffer
1 x 20 mL
8-Well Assay Strips (with Frame)
12 x 1 Unit
8-Well Strip Caps
12 x 1 Unit
96-Well PCR Plate
1 x 1 Unit
Adhesive Covering Film
2 x 1 Unit
Anti-RNA Polymerase II (1 mg/ml)
1 x 16 µL
ChIP Buffer
1 x 12 mL
DNA Release Buffer
1 x 28 mL
GAPDH Primer - Forward (20 µM)
1 x 16 µL
GAPDH Primer - Reverse (20 µM)
1 x 16 µL
Non-Immune IgG (1 mg/ml)
1 x 20 µL
Proteinase K (10 mg/mL)
1 x 110 µL
Sonication Frame
1 x 1 Unit

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Abcam's ChIP Kit - One Step (ab117138) enables the user to enrich a chromatin fraction containing specific DNA sequences from isolated chromatin to be able to investigate the interaction between proteins and DNA.

Key facts

Assay time

5h

Assay type

Quantitative

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Abcam's ChIP Kit - One Step (ab117138) enables the user to enrich a chromatin fraction containing specific DNA sequences from isolated chromatin to be able to investigate the interaction between proteins and DNA. For best result, we recommend the use of Chromatin Extraction Kit (ab117152) for sample preparation.

Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In mammalian cells, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.

**Primers** The GAPDH primers provided with the kit are for the human sequence. If using the kit with a difference species, GAPDH primers for that species will need to be acquired.  **ChIP assay products and guides** Find more resources and products, products, and other .

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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