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Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing.

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Assay time

6h

Assay type

Quantitative

Reactive species

Plants

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Reactivity data

Application
ChIP
Reactivity
Reacts
Dilution info
-
Notes

-

Images

What's included?

48 Test
Components
100X Protease Inhibitor Cocktail
1 x 50 µL
5X Lysis Buffer I
1 x 24 mL
8-Well Assay Strips (with Frame)
6 x 1 Unit
8-Well Strip Caps
6 x 1 Unit
Anti-H3K9me2 (1 mg/mL)
1 x 8 µL
Antibody Buffer
1 x 30 mL
Binding Buffer
1 x 8 mL
ChIP Dilution Buffer
1 x 6 mL
DNA Release Buffer
2 x 2 mL
Elution Buffer
1 x 1.2 mL
F-Collection Tube
50 x 1 Unit
F-Spin Column
50 x 1 Unit
Lysis Buffer II
1 x 6 mL
Lysis Buffer III
1 x 4 mL
Lysis Buffer IV
1 x 5 mL
Normal Mouse IgG (1 mg/mL)
1 x 10 µL
Proteinase K (10 mg/mL)
1 x 50 µL
Reverse Buffer
2 x 2 mL
Wash Buffer
2 x 28 mL

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Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing.

Key facts

Assay time

6h

Assay type

Quantitative

Reactive species

Plants

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In plants, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.

Abcam's ChIP Kit - Plants (ab117137) offers an advantageous tool for identifying direct genome-wide associations between specific regulatory proteins and their target genes in plant cells within 6 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing and southern blot, as well as DNA microarray.

This kit is designed for 24 or 48 ChIP reactions, not for 24 or 48 samples. The standard protocol of the kit allows for performing 8 reactions with one sample. For testing more samples, the amount of each sample should be reduced. The amount of each reagent used for chromatin preparation should be also proportionally reduced.

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Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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