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Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing.

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Images

ChIP - ChIP Kit - Plants (AB117137), expandable thumbnail
  • ChIP - ChIP Kit - Plants (AB117137), expandable thumbnail

Publications

Key facts

Assay time
6h
Assay type
Quantitative
Reactive species
Plants

Reactivity data

Application
ChIP
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

What's included?

48 Test
Components
100X Protease Inhibitor Cocktail
1 x 50 µL
5X Lysis Buffer I
1 x 24 mL
8-Well Assay Strips (with Frame)
6 x 1 Unit
8-Well Strip Caps
6 x 1 Unit
Anti-H3K9me2 (1 mg/mL)
1 x 8 µL
Antibody Buffer
1 x 30 mL
Binding Buffer
1 x 8 mL
ChIP Dilution Buffer
1 x 6 mL
DNA Release Buffer
2 x 2 mL
Elution Buffer
1 x 1.2 mL
F-Collection Tube
50 x 1 Unit
F-Spin Column
50 x 1 Unit
Lysis Buffer II
1 x 6 mL
Lysis Buffer III
1 x 4 mL
Lysis Buffer IV
1 x 5 mL
Normal Mouse IgG (1 mg/mL)
1 x 10 µL
Proteinase K (10 mg/mL)
1 x 50 µL
Reverse Buffer
2 x 2 mL
Wash Buffer
2 x 28 mL

Recommended products

Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing.

Key facts

Assay time
6h
Assay type
Quantitative
Reactive species
Plants

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In plants, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.

Abcam's ChIP Kit - Plants (ab117137) offers an advantageous tool for identifying direct genome-wide associations between specific regulatory proteins and their target genes in plant cells within 6 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing and southern blot, as well as DNA microarray.

This kit is designed for 24 or 48 ChIP reactions, not for 24 or 48 samples. The standard protocol of the kit allows for performing 8 reactions with one sample. For testing more samples, the amount of each sample should be reduced. The amount of each reagent used for chromatin preparation should be also proportionally reduced.

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Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • ChIP - ChIP Kit - Plants (ab117137), expandable thumbnail
    Image from Hufnagel et al., Nat Commun.;11(1):492. doi: 10.1038/s41467-019-14197-9. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    ChIP - ChIP Kit - Plants (ab117137)

    Repeated elements abundance in White Lupin genome. Chromatin immunoprecipitation experiments were done with ChIP Kit - Plants (ab117137). Sonicated chromatin-DNA ranging from 200-1000 bp was immunoprecipitated using anti-LalbCENH3. LalbCENH3-ChIPseq reads mapped against the first 100 RepeatExplorer clusters of the White Lupin genome. The main centromeric sequences found in LalbCENH3-ChIPseq are highlighted.

  • ChIP - ChIP Kit - Plants (ab117137), expandable thumbnail
    Image from Kim et al., Front Plant Sci.,11:589707. doi: 10.3389/fpls.2020.589707. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    ChIP - ChIP Kit - Plants (ab117137)

    ORE1 promoter-binding activity of GIGANTEA in the elf4 mutant relative to that in the wild type at amplicons 1, 2, and 3. ChIP assays were performed using the ChIP Kit-Plants (ab117137). Chromatin was immunoprecipitated using anti-GFP antibody (Anti-GFP antibody ab290)-bound assay plate for 90 min. The resulting immunoprecipitated DNA was subjected to qPCR to examine the enrichment of target genes. Three biological replicates were performed. Asterisks indicate significant differences (*p < 0.05, **p < 0.01, ***p < 0.005; Student's t-test).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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