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AB117137

ChIP Kit - Plants

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(10 Publications)

Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing.
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ChIP - ChIP Kit - Plants (AB117137)
  • ChIP

PubMed

ChIP - ChIP Kit - Plants (AB117137)

ORE1 promoter-binding activity of GIGANTEA in the elf4 mutant relative to that in the wild type at amplicons 1, 2, and 3. ChIP assays were performed using the ChIP Kit-Plants (ab117137). Chromatin was immunoprecipitated using anti-GFP antibody (ab290)-bound assay plate for 90 min. The resulting immunoprecipitated DNA was subjected to qPCR to examine the enrichment of target genes. Three biological replicates were performed. Asterisks indicate significant differences (*p < 0.05, **p < 0.01, ***p < 0.005; Student's t-test).

Image from Kim et al., Front Plant Sci.,11:589707. doi: 10.3389/fpls.2020.589707. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

ChIP - ChIP Kit - Plants (AB117137)
  • ChIP

PubMed

ChIP - ChIP Kit - Plants (AB117137)

Repeated elements abundance in White Lupin genome. Chromatin immunoprecipitation experiments were done with ChIP Kit - Plants (ab117137). Sonicated chromatin-DNA ranging from 200-1000 bp was immunoprecipitated using anti-LalbCENH3. LalbCENH3-ChIPseq reads mapped against the first 100 RepeatExplorer clusters of the White Lupin genome. The main centromeric sequences found in LalbCENH3-ChIPseq are highlighted.

Image from Hufnagel et al., Nat Commun.;11(1):492. doi: 10.1038/s41467-019-14197-9. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

Key facts

Assay time

6h

Assay type

Quantitative

Reacts with

Plants

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ChIP": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In plants, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.

Abcam's ChIP Kit - Plants (ab117137) offers an advantageous tool for identifying direct genome-wide associations between specific regulatory proteins and their target genes in plant cells within 6 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing and southern blot, as well as DNA microarray.

This kit is designed for 24 or 48 ChIP reactions, not for 24 or 48 samples. The standard protocol of the kit allows for performing 8 reactions with one sample. For testing more samples, the amount of each sample should be reduced. The amount of each reagent used for chromatin preparation should be also proportionally reduced.

**ChIP assay products and guides**Find more resources and products, products, and other .

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in plant science 14:1128002 PubMed36844077

2023

Grapevine bZIP transcription factor bZIP45 regulates and confers drought tolerance in .

Applications

Unspecified application

Species

Unspecified reactive species

Shuaike Niu,Xiangyang Gu,Qian Zhang,Xuemin Tian,Zhan Chen,Jingru Liu,Xiaoju Wei,Chengxiang Yan,Ziwen Liu,Xiaoji Wang,Zhengge Zhu

Tree physiology 43:675-689 PubMed36637421

2023

Systematic evaluation of chromatin immunoprecipitation sequencing to study histone occupancy in dormancy transitions of grapevine buds.

Applications

Unspecified application

Species

Unspecified reactive species

Dina Hermawaty,Jonathan Cahn,Ryan Lister,Michael J Considine

The New phytologist 235:1470-1485 PubMed35510737

2022

PEAPOD repressors modulate and coordinate developmental responses to light intensity in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Derek W R White

The Plant journal : for cell and molecular biology 107:1631-1647 PubMed34219317

2021

Open chromatin in grapevine marks candidate CREs and with other chromatin features correlates with gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel Schwope,Gabriele Magris,Mara Miculan,Eleonora Paparelli,Mirko Celii,Aldo Tocci,Fabio Marroni,Alice Fornasiero,Emanuele De Paoli,Michele Morgante

Frontiers in plant science 11:589707 PubMed33329652

2020

Subcellular Localization of GIGANTEA Regulates the Timing of Leaf Senescence and Flowering in .

Applications

Unspecified application

Species

Unspecified reactive species

Hyunmin Kim,Su Jin Park,Yumi Kim,Hong Gil Nam

Current biology : CB 30:4780-4788.e5 PubMed33007250

2020

Three STIGMA AND STYLE STYLISTs Pattern the Fine Architectures of Apical Gynoecium and Are Critical for Male Gametophyte-Pistil Interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Wenwei Li,Xiaorong Huang,Jie Zou,Jianjun Wu,Hengwu Jiao,Xiongbo Peng,Meng-Xiang Sun

Frontiers in plant science 11:532771 PubMed33013960

2020

Seed-Specific Overexpression of and Improves Seed Dormancy and Grain Size in Rice.

Applications

Unspecified application

Species

Unspecified reactive species

Miaomiao Qin,Yan Zhang,Yanmei Yang,Chunbo Miao,Shenkui Liu

Nature communications 11:492 PubMed31980615

2020

High-quality genome sequence of white lupin provides insight into soil exploration and seed quality.

Applications

Unspecified application

Species

Unspecified reactive species

Bárbara Hufnagel,André Marques,Alexandre Soriano,Laurence Marquès,Fanchon Divol,Patrick Doumas,Erika Sallet,Davide Mancinotti,Sébastien Carrere,William Marande,Sandrine Arribat,Jean Keller,Cécile Huneau,Thomas Blein,Delphine Aimé,Malika Laguerre,Jemma Taylor,Veit Schubert,Matthew Nelson,Fernando Geu-Flores,Martin Crespi,Karine Gallardo,Pierre-Marc Delaux,Jérôme Salse,Hélène Bergès,Romain Guyot,Jérôme Gouzy,Benjamin Péret

Nature communications 10:3484 PubMed31375676

2019

Maternal control of suspensor programmed cell death via gibberellin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ce Shi,Pan Luo,Yu-Ting Du,Hong Chen,Xiaorong Huang,Tian-He Cheng,An Luo,Hong-Ju Li,Wei-Cai Yang,Peng Zhao,Meng-Xiang Sun

The Plant journal : for cell and molecular biology 84:111-24 PubMed26255869

2015

Chromatin state analysis of the barley epigenome reveals a higher-order structure defined by H3K27me1 and H3K27me3 abundance.

Applications

Unspecified application

Species

Unspecified reactive species

Katie Baker,Taniya Dhillon,Isabelle Colas,Nicola Cook,Iain Milne,Linda Milne,Micha Bayer,Andrew J Flavell
View all publications
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