ChIP Kit (Transcription factors, ChIP-seq)
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-Includes all buffers and reagents for chromatin shearing, immunoprecipitation, and DNA isolation
- Contains a negative (IgG) and a positive (CTCF) control antibody- Protein A magnetic beads are used to isolate the protein-DNA complexes of interest
- Eluted DNA suitable for Next-Generation Sequencing (NGS)
- ChIP-seq
Lab
ChIP-sequencing - ChIP Kit (Transcription factors, ChIP-seq) (AB270813)
ChIP-sequencing - Anti-RNA polII CTD S2P antibody [EPR18855-87] - ChIP Grade (ab238146).
Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 μg of chromatin and 4 μg of ab238146. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813).
- ChIP-seq
Lab
ChIP-sequencing - ChIP Kit (Transcription factors, ChIP-seq) (AB270813)
ChIP-sequencing - Anti-SP1 antibody [EPR22648-50] - ChIP Grade (ab231778).
Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 μg of chromatin and 4 μg of ab231778. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813).
- ChIP-seq
Lab
ChIP-sequencing - ChIP Kit (Transcription factors, ChIP-seq) (AB270813)
ChIP-sequencing - Anti-TBP antibody [EPR21954] - ChIP Grade (ab220788).
Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 μg of chromatin and 4 μg of ab220788. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813).
- ChIP-seq
Lab
ChIP-sequencing - ChIP Kit (Transcription factors, ChIP-seq) (AB270813)
ChIP-sequencing - Anti-CTCF antibody [EPR18253] - ChIP Grade (ab188408).
Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 μg of chromatin and 4 μg of ab188408. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813).
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Product details
ChIP Kit (Transcription factors, ChIP-seq) (ab270813) is a highly validated solution for robust transcription factor and other non-histone proteins ChIP-seq results and contains everything you need for start-to-finish ChIP prior to Next-Generation Sequencing. This complete solution contains all buffers and reagents for cell lysis, chromatin shearing, immunoprecipitation, and DNA purification. This kit contains positive and negative control antibodies (CTCF and IgG, respectively) as well as positive and negative control PCR primers pairs (H19 and Myoglobin exon 2, respectively).
Sample size:
Cells (4,000,000 cells per IP)
Tissues (30 mg of tissue per IP)
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download chromatinShearingProtocolWebsite|en
- Download chipSequencingBookletWebsite|en
- Download pre-chipAssayPreparationWebsite|en
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com