ChIP Kit (Transcription factors, ChIP-seq) (ab270813) is a highly validated solution for robust transcription factor and other non-histone proteins ChIP-seq results and contains everything you need for start-to-finish ChIP prior to Next-Generation Sequencing.
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ChIP Kit (Transcription factors, ChIP-seq) (ab270813) is a highly validated solution for robust transcription factor and other non-histone proteins ChIP-seq results and contains everything you need for start-to-finish ChIP prior to Next-Generation Sequencing.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
ChIP Kit (Transcription factors, ChIP-seq) (ab270813) is a highly validated solution for robust transcription factor and other non-histone proteins ChIP-seq results and contains everything you need for start-to-finish ChIP prior to Next-Generation Sequencing. This complete solution contains all buffers and reagents for cell lysis, chromatin shearing, immunoprecipitation, and DNA purification. This kit contains positive and negative control antibodies (CTCF and IgG, respectively) as well as positive and negative control PCR primers pairs (H19 and Myoglobin exon 2, respectively).
Sample size:
Cells (4,000,000 cells per IP)
Tissues (30 mg of tissue per IP)
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4 product images
ChIP-sequencing - ChIP Kit (Transcription factors, ChIP-seq) (ab270813)
ChIP-sequencing - Anti-RNA polII CTD S2P antibody [EPR18855-87] - ChIP Grade (Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [EPR18855-87] - ChIP Grade ab238146).
Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 μg of chromatin and 4 μg of Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [EPR18855-87] - ChIP Grade ab238146. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813).
ChIP-sequencing - ChIP Kit (Transcription factors, ChIP-seq) (ab270813)
ChIP-sequencing - Anti-SP1 antibody [EPR22648-50] - ChIP Grade (Anti-SP1 antibody [EPR22648-50] - ChIP Grade ab231778).
Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 μg of chromatin and 4 μg of Anti-SP1 antibody [EPR22648-50] - ChIP Grade ab231778. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813).
ChIP-sequencing - ChIP Kit (Transcription factors, ChIP-seq) (ab270813)
ChIP-sequencing - Anti-TBP antibody [EPR21954] - ChIP Grade (Anti-TATA binding protein TBP antibody [EPR21954] - Loading Control and ChIP Grade ab220788).
Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 μg of chromatin and 4 μg of Anti-TATA binding protein TBP antibody [EPR21954] - Loading Control and ChIP Grade ab220788. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813).
ChIP-sequencing - ChIP Kit (Transcription factors, ChIP-seq) (ab270813)
ChIP-sequencing - Anti-CTCF antibody [EPR18253] - ChIP Grade (Anti-CTCF antibody [EPR18253] - ChIP Grade ab188408).
Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 μg of chromatin and 4 μg of Anti-CTCF antibody [EPR18253] - ChIP Grade ab188408. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813).
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