Skip to main content

ChIP Kit (Transcription factors, ChIP-seq) (ab270813) is a highly validated solution for robust transcription factor and other non-histone proteins ChIP-seq results and contains everything you need for start-to-finish ChIP prior to Next-Generation Sequencing.

Be the first to review this product! Submit a review

Images

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

What's included?

24 Test
Components
5% BSA (DNA free)
1 x 800 µL
5x ChIP Buffer C1b
1 x 6.9 mL
Buffer C
1 x 1.6 mL
Carrier
1 x 72 µL
ChIP-seq grade CTCF antibody
1 x 8 µg
ChIP-seq grade H19 imprinting control region primer pair (human)
1 x 96 µL
ChIP-seq grade Myoglobin exon 2 primer pair (human)
1 x 96 µL
ChIP-seq grade water
1 x 26.6 mL
Elution Buffer E1
1 x 3.4 mL
Elution-buffer E2
1 x 144 µL
Fixation buffer
1 x 8 mL
Glycine
1 x 8.8 mL
Lysis Buffer L1b
1 x 100 mL
Lysis Buffer L2
1 x 60 mL
Protease inhibitor cocktail
1 x 80 µL
Protein A-coated magnetic beads
1 x 720 µL
Purification Beads
1 x 400 µL
Rabbit IgG
1 x 8 µg
Shearing Buffer S1b
1 x 6.7 mL
Wash buffer 1 w/o iso-propanol
1 x 2 mL
Wash buffer 2 w/o iso-propanol
1 x 2 mL
Wash buffer W1
1 x 8.4 mL
Wash buffer W2
1 x 8.4 mL
Wash buffer W3
1 x 8.4 mL
Wash buffer W4
1 x 8.4 mL

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

ChIP Kit (Transcription factors, ChIP-seq) (ab270813) is a highly validated solution for robust transcription factor and other non-histone proteins ChIP-seq results and contains everything you need for start-to-finish ChIP prior to Next-Generation Sequencing.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

ChIP Kit (Transcription factors, ChIP-seq) (ab270813) is a highly validated solution for robust transcription factor and other non-histone proteins ChIP-seq results and contains everything you need for start-to-finish ChIP prior to Next-Generation Sequencing. This complete solution contains all buffers and reagents for cell lysis, chromatin shearing, immunoprecipitation, and DNA purification. This kit contains positive and negative control antibodies (CTCF and IgG, respectively) as well as positive and negative control PCR primers pairs (H19 and Myoglobin exon 2, respectively).

Sample size:

Cells (4,000,000 cells per IP)

Tissues (30 mg of tissue per IP)

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • ChIP-sequencing - ChIP Kit (Transcription factors, ChIP-seq) (ab270813), expandable thumbnail

    ChIP-sequencing - ChIP Kit (Transcription factors, ChIP-seq) (ab270813)

    ChIP-sequencing - Anti-RNA polII CTD S2P antibody [EPR18855-87] - ChIP Grade (ab238146).

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 μg of chromatin and 4 μg of ab238146. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813).

  • ChIP-sequencing - ChIP Kit (Transcription factors, ChIP-seq) (ab270813), expandable thumbnail

    ChIP-sequencing - ChIP Kit (Transcription factors, ChIP-seq) (ab270813)

    ChIP-sequencing - Anti-SP1 antibody [EPR22648-50] - ChIP Grade (ab231778).

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 μg of chromatin and 4 μg of ab231778. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813).

  • ChIP-sequencing - ChIP Kit (Transcription factors, ChIP-seq) (ab270813), expandable thumbnail

    ChIP-sequencing - ChIP Kit (Transcription factors, ChIP-seq) (ab270813)

    ChIP-sequencing - Anti-TBP antibody [EPR21954] - ChIP Grade (ab220788).

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 μg of chromatin and 4 μg of ab220788. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813).

  • ChIP-sequencing - ChIP Kit (Transcription factors, ChIP-seq) (ab270813), expandable thumbnail

    ChIP-sequencing - ChIP Kit (Transcription factors, ChIP-seq) (ab270813)

    ChIP-sequencing - Anti-CTCF antibody [EPR18253] - ChIP Grade (ab188408).

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 μg of chromatin and 4 μg of ab188408. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ab270813).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com