AB185908

ChIP-Seq High Sensitivity Kit

Name: ChIP-Seq High Sensitivity Kit (ab185908) | Abcam
Brand: Abcam Limited
SKU: AB185908
Price: 1270 USD
Availability: InStock

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(4 Publications)

ab185908 is a complete set of reagents required for carrying out a successful ChIP-Seq starting from mammalian cells or tissues.

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ChIP - ChIP-Seq High Sensitivity Kit (AB185908)

ChIP

Supplier Data

ChIP - ChIP-Seq High Sensitivity Kit (AB185908)

Sample results.

A ChIP-sequencing library was prepared using ChIP-Seq High Sensitivity Kit (ab185908) from rat heart chromatin and polyclonal antibodies against H3K4me3, H3(K9/14)ac and, H3K27me3. Sequencing was carried out on an Illumina HiSeq2500. Bioinformatics analysis of ChIP-seq is performed utilizing Bowtie and MACS.

ChIP-sequencing - ChIP-Seq High Sensitivity Kit (AB185908)

ChIP-seq

Unknown

ChIP-sequencing - ChIP-Seq High Sensitivity Kit (AB185908)

High sensitive ChIP.

The sheared chromatin isolated from different number of MBD-231 cells was used for ChIP-qPCR analysis of RNA polymerase II enrichment in GAPDH promoters.

Spectrum - ChIP-Seq High Sensitivity Kit (AB185908)

Spectrum

Supplier Data

Spectrum - ChIP-Seq High Sensitivity Kit (AB185908)

Size distribution of library fragments.

Ten nanograms of DNA was ChIPed by RNA polymerase II enrichment and used for DNA library preparation.

Key facts

Assay time

Sample types

Tissue, Suspension cells, Adherent cells

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ChIP-seq": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

ab185908 is a complete set of reagents required for carrying out a successful ChIP-Seq starting from mammalian cells or tissues. The kit is designed to selectively enrich a chromatin fraction containing specific DNA sequences from various species, particularly mammals, and to prepare a ChIP-Seq library for next generation sequencing using Illumina® platforms such as Illumina® Genome Analyzer II, HiSeq and MiSeq systems. The optimized protocol and components of the kit allow capture of low abundance protein/DNA complexes with minimized non-specific background levels and the ability to construct both non-barcoded (singleplexed) and barcoded (multiplexed) ChIP-Seq libraries quickly with reduced bias.

Starting Materials:
Starting materials can include various tissue or cell samples such as culture cells from a flask or plate, fresh and frozen tissues, etc.

Input Amount of Tissue/Cells:

In general, the amount of cells and tissues for each reaction can be 1 x 10⁵ to 1 x 10⁶ and 5 mg to 50 mg, respectively. For optimal preparation, the input amount should be 4 to 5 x 10⁵cells or 20 to 30 mg tissues so that the amount of DNA enriched from the ChIP reaction can range from at least 1 ng to 100 ng.

Illumina® is a registered trademark of Illumina, Inc.

Protein-DNA interaction plays a critical role for cellular functions such as signal transduction, gene transcription, chromosome segregation, DNA replication and recombination, and epigenetic silencing. Identifying the genetic targets of DNA binding proteins and knowing the mechanisms of protein-DNA interaction on a genome-wide scale is important for understanding cellular processes. Chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) offers an advantageous tool for studying genome-wide protein-DNA interactions. It allows for detection that a specific protein binds to specific sequences in living cells. In particular, ChIP antibodies targeted against various transcriptional factors (TF) for genome-wide transcription factor binding site analysis by Chip-Seq is in high demand. Such analysis requires that ChIPed DNA contain minimal background for reliably identifying true TF-enriched regions. Currently used ChIP-Seq methods play an important role in identifying genome-wide protein-DNA interaction. The ChIP-Seq High Sensitivity Kit combines microplate-based ultra ChIP and high sensitive DNA library construction technologies.

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What's included?

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Reverse (20 µM)", "number":"AB185908-CMP28", "productcode":"" }, { "size":"1 x 2 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB185908-CMP01", "productcode":"" }, { "size":"1 x 5 µL", "name":"GAPDH Primer - Forward (20 µM)", "number":"AB185908-CMP27", "productcode":"" }, { "size":"1 x 13 µL", "name":"End Polishing Enhancer", "number":"AB185908-CMP20", "productcode":"" }, { "size":"1 x 13 µL", "name":"End Polishing Enzyme Mix", "number":"AB185908-CMP21", "productcode":"" }, { "size":"1 x 160 µL", "name":"2X HiFi PCR Master Mix", "number":"AB185908-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"ChIP Buffer", "number":"AB185908-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Product protocols

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Target data

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & therapy 25:2299288 PubMed38178596

2024

WYC-209 inhibited GC malignant progression by down-regulating WNT4 through RARα.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenyuan Qian,Wenfa Lin,Xufan Cai,Jianzhang Wu,Kun Ke,Zaiyuan Ye,Fang Wu

Pathology, research and practice 238:154076 PubMed36055087

2022

WDR5 drives the development of cervical squamous cell carcinoma by inducing epithelial-mesenchymal transition and cancer-associated fibroblasts formation.

Applications

Unspecified application

Species

Unspecified reactive species

Fangli Sun,Linmei Mo,Ying Lan,Qiuping Lu,Nengxian Wu,Honglin Song

Scientific reports 11:21500 PubMed34728675

2021

Integrative genome-wide analysis of dopaminergic neuron-specific PARIS expression in Drosophila dissects recognition of multiple PPAR-γ associated gene regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Volkan Yazar,Sung-Ung Kang,Shinwon Ha,Valina L Dawson,Ted M Dawson

Journal of hepatocellular carcinoma 8:333-348 PubMed33977095

2021

LncRNA-CCDC144NL-AS1 Promotes the Development of Hepatocellular Carcinoma by Inducing WDR5 Expression via Sponging miR-940.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Zhang,Hongyu Zhang,Shuhuan Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

ChIP-Seq High Sensitivity Kit

ChIP - ChIP-Seq High Sensitivity Kit (AB185908)

ChIP-sequencing - ChIP-Seq High Sensitivity Kit (AB185908)

Spectrum - ChIP-Seq High Sensitivity Kit (AB185908)

Key facts

Assay time

Sample types

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (4)

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