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ab185908 is a complete set of reagents required for carrying out a successful ChIP-Seq starting from mammalian cells or tissues.

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Assay time

7h

Sample type

Tissue, Suspension cells, Adherent cells

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Reactivity data

Application
ChIP-seq
Reactivity
Reacts
Dilution info
-
Notes

-

Images

What's included?

24 Test
Components
1000X Protease Inhibitor Cocktail
1 x 30 µL
10X End Polishing Buffer
1 x 60 µL
2X HiFi PCR Master Mix
1 x 320 µL
2X Ligation Buffer
1 x 500 µL
8-Well Assay Strips (with Frame)
1 x 4 Unit
8-Well Strip Caps
1 x 4 Unit
Adaptors (50 μM)
1 x 30 µL
Adhesive 8-Well Strip Film
1 x 8 Unit
Anti-RNA Polymerase II
1 x 10 µL
Antibody Buffer
1 x 2 mL
Blocker Solution
1 x 2 mL
ChIP Buffer
1 x 12 mL
DNA Binding Solution
1 x 14 mL
DNA Elution Buffer
1 x 1 mL
DNA Release Buffer
1 x 14 mL
Elution Buffer
1 x 2 mL
End Polishing Enhancer
1 x 26 µL
End Polishing Enzyme Mix
1 x 26 µL
Enrichment Enhancer
1 x 50 µL
F-Collection Tube
1 x 30 Unit
F-Spin Column
1 x 30 Unit
GAPDH Primer - Forward (20 µM)
1 x 10 µL
GAPDH Primer - Reverse (20 µM)
1 x 10 µL
Lysis Buffer
1 x 14 mL
MQ Binding Beads
2 x 1.6 mL
Non-Immune IgG (1 mg/ml)
1 x 10 µL
Primer I (10 μM)
1 x 30 µL
Primer U (10 μM)
1 x 30 µL
Proteinase K (10 mg/mL)
1 x 60 µL
Rnase A
1 x 30 µL
T4 DNA Ligase
1 x 30 µL
Wash Buffer
1 x 25 mL

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ab185908 is a complete set of reagents required for carrying out a successful ChIP-Seq starting from mammalian cells or tissues.

Key facts

Assay time

7h

Sample type

Tissue, Suspension cells, Adherent cells

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

ab185908 is a complete set of reagents required for carrying out a successful ChIP-Seq starting from mammalian cells or tissues. The kit is designed to selectively enrich a chromatin fraction containing specific DNA sequences from various species, particularly mammals, and to prepare a ChIP-Seq library for next generation sequencing using Illumina® platforms such as Illumina® Genome Analyzer II, HiSeq and MiSeq systems. The optimized protocol and components of the kit allow capture of low abundance protein/DNA complexes with minimized non-specific background levels and the ability to construct both non-barcoded (singleplexed) and barcoded (multiplexed) ChIP-Seq libraries quickly with reduced bias.

Starting Materials:
Starting materials can include various tissue or cell samples such as culture cells from a flask or plate, fresh and frozen tissues, etc.

Input Amount of Tissue/Cells:

In general, the amount of cells and tissues for each reaction can be 1 x 105 to 1 x 106 and 5 mg to 50 mg, respectively. For optimal preparation, the input amount should be 4 to 5 x 105cells or 20 to 30 mg tissues so that the amount of DNA enriched from the ChIP reaction can range from at least 1 ng to 100 ng.

Illumina® is a registered trademark of Illumina, Inc.

Protein-DNA interaction plays a critical role for cellular functions such as signal transduction, gene transcription, chromosome segregation, DNA replication and recombination, and epigenetic silencing. Identifying the genetic targets of DNA binding proteins and knowing the mechanisms of protein-DNA interaction on a genome-wide scale is important for understanding cellular processes. Chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) offers an advantageous tool for studying genome-wide protein-DNA interactions. It allows for detection that a specific protein binds to specific sequences in living cells. In particular, ChIP antibodies targeted against various transcriptional factors (TF) for genome-wide transcription factor binding site analysis by Chip-Seq is in high demand. Such analysis requires that ChIPed DNA contain minimal background for reliably identifying true TF-enriched regions. Currently used ChIP-Seq methods play an important role in identifying genome-wide protein-DNA interaction. The ChIP-Seq High Sensitivity Kit combines microplate-based ultra ChIP and high sensitive DNA library construction technologies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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