AB117152

Chromatin Extraction Kit

Name: Chromatin extraction kit - 100 tests (ab117152) | Abcam
Brand: Abcam Limited
SKU: AB117152
Price: 285 USD
Availability: InStock

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(51 Publications)

60-minute chromatin extraction kit is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells and tissues in a simple and rapid format.

- Cited in over 50 publications

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Functional Studies - Chromatin Extraction Kit (AB117152)

FuncS

Supplier Data

Functional Studies - Chromatin Extraction Kit (AB117152)

ChIP analysis of RNA polymerase II enriched in GAPDH and MLH1 promoters with chromatin extract prepared from formaldehyde fixed colon cancer cells (2x10⁵) using ab117152.

FuncS

Unknown

Functional Studies - Chromatin Extraction Kit (AB117152)

Chromatin was extracted from 3x10⁶ mouse neuroblastoma cells, fixed for 10min at RT in 0.1% formaldehyde. Cells were sonicated before last centrifugation, and following centrifugation, cells were treated with proteinase K & RNAse A.
Chromatin yield was 4 μg chromatin/10⁶ cells.

Lane 1 : 1kb DNA ladder
Lane 2 : 300 - 650bp DNA fragments

Image courtesy of anonymous Abreview

Key facts

Assay time

Sample types

Tissue, Suspension cells, Adherent cells

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Product details

60-minute chromatin extraction kit is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells and tissues in a simple and rapid format.

Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab117152-100Test", "assetComponentDetails": [ { "size":"1 x 110 µL", "name":"1000X Protease Inhibitor Cocktail", "number":"AB117152-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Extraction Buffer", "number":"AB117152-CMP03", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromatin Buffer", "number":"AB117152-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"10X Lysis Buffer", "number":"AB117152-CMP01", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Product protocols

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Target data

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Publications (51)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & therapy 25:2329372 PubMed38494680

2024

CPT1A mediates the succinylation of SP5 which activates transcription of PDPK1 to promote the viability and glycolysis of prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shufeng Liu,Xiaoguang Chen,Liqi Zhang,Bo Lu

Nature communications 15:1579 PubMed38383676

2024

Oncogenic c-Myc induces replication stress by increasing cohesins chromatin occupancy in a CTCF-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Peripolli,Leticia Meneguello,Chiara Perrod,Tanya Singh,Harshil Patel,Sazia T Rahman,Koshiro Kiso,Peter Thorpe,Vincenzo Calvanese,Cosetta Bertoli,Robertus A M de Bruin

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2302368 PubMed37949681

2023

Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Huaiyuan Liang,Chunguang Yang,Ruijiang Zeng,Yingqiu Song,Jianxi Wang,Wei Xiong,Binyuan Yan,Xin Jin

eLife 12: PubMed37603466

2023

The negative adipogenesis regulator is transcriptionally regulated by (TIS7) and translationally by its orthologue (SKMc15).

Applications

Unspecified application

Species

Unspecified reactive species

Ilja Vietor,Domagoj Cikes,Kati Piironen,Theodora Vasakou,David Heimdörfer,Ronald Gstir,Matthias David Erlacher,Ivan Tancevski,Philipp Eller,Egon Demetz,Michael W Hess,Volker Kuhn,Gerald Degenhart,Jan Rozman,Martin Klingenspor,Martin Hrabe de Angelis,Taras Valovka,Lukas A Huber

Bone research 11:30 PubMed37280207

2023

Super enhancers targeting ZBTB16 in osteogenesis protect against osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhui Yu,Zhongyu Xie,Jinteng Li,Jiajie Lin,Zepeng Su,Yunshu Che,Feng Ye,Zhaoqiang Zhang,Peitao Xu,Yipeng Zeng,Xiaojun Xu,Zhikun Li,Pei Feng,Rujia Mi,Yanfeng Wu,Huiyong Shen

International journal of molecular sciences 24: PubMed37239999

2023

Nuclear DJ-1 Regulates DNA Damage Repair via the Regulation of PARP1 Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Zhong-Xuan Wang,Yi Liu,Yao-Lin Li,Qiao Wei,Rong-Rong Lin,Ruiqing Kang,Yang Ruan,Zhi-Hao Lin,Nai-Jia Xue,Bao-Rong Zhang,Jia-Li Pu

Birth defects research 115:770-781 PubMed36899481

2023

Effects of ketamine-induced H3K9 hypoacetylation during pregnancy on cardiogenesis of mouse offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Yujuan Yu,Junjun Quan,Mou Zou,Wei Zhao,Yujuan Su,Ying Xu

Journal of cellular physiology 238:992-1005 PubMed36852589

2023

Obesity-mediated upregulation of the YAP/IL33 signaling axis promotes aggressiveness and induces an immunosuppressive tumor microenvironment in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Zih Dai,Ching-Chieh Yang,Pei-Wei Shueng,Yen-Ju Wang,Chen-Shiuan Huang,Yi-Chun Chao,Cheng-Hsun Chen,Cheng-Wei Lin

Journal of nanobiotechnology 21:37 PubMed36732759

2023

Apoptotic tumor cell-derived microparticles loading Napabucasin inhibit CSCs and synergistic immune therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Boping Jing,Feng Guo,Rui An,Yu Gao,Yuman Li,Yuji Xie,Jing Wang,Yihan Chen,He Li,Tang Gao,Qiaofeng Jin,Li Zhang,Mingxing Xie

Cell death & disease 14:47 PubMed36670097

2023

TRIM15 forms a regulatory loop with the AKT/FOXO1 axis and LASP1 to modulate the sensitivity of HCC cells to TKIs.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Yang,Xin Jin,Xingchao Liu,Gang Wu,Wenhao Yang,Beichuan Pang,Jipeng Jiang,Dongxu Liao,Yu Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Chromatin Extraction Kit

Functional Studies - Chromatin Extraction Kit (AB117152)

Functional Studies - Chromatin Extraction Kit (AB117152)

Key facts

Assay time

Sample types

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (51)

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