60-minute chromatin extraction kit is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells and tissues in a simple and rapid format.
- Cited in over 50 publications
Images
Publications
Filter by
- Cancer biology & therapy 25:23293722024CPT1A mediates the succinylation of SP5 which activates transcription of PDPK1 to promote the viability and glycolysis of prostate cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShufeng Liu,Xiaoguang Chen,Liqi Zhang,Bo LuPubMed 38494680
- Nature communications 15:15792024Oncogenic c-Myc induces replication stress by increasing cohesins chromatin occupancy in a CTCF-dependent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSilvia Peripolli,Leticia Meneguello,Chiara Perrod,Tanya Singh,Harshil Patel,Sazia T Rahman,Koshiro Kiso,Peter Thorpe,Vincenzo Calvanese,Cosetta Bertoli,Robertus A M de BruinPubMed 38383676
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e23023682023Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuaiyuan Liang,Chunguang Yang,Ruijiang Zeng,Yingqiu Song,Jianxi Wang,Wei Xiong,Binyuan Yan,Xin JinPubMed 37949681
- eLife 12:2023The negative adipogenesis regulator is transcriptionally regulated by (TIS7) and translationally by its orthologue (SKMc15).Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlja Vietor,Domagoj Cikes,Kati Piironen,Theodora Vasakou,David Heimdörfer,Ronald Gstir,Matthias David Erlacher,Ivan Tancevski,Philipp Eller,Egon Demetz,Michael W Hess,Volker Kuhn,Gerald Degenhart,Jan Rozman,Martin Klingenspor,Martin Hrabe de Angelis,Taras Valovka,Lukas A HuberPubMed 37603466
- Bone research 11:302023Super enhancers targeting ZBTB16 in osteogenesis protect against osteoporosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenhui Yu,Zhongyu Xie,Jinteng Li,Jiajie Lin,Zepeng Su,Yunshu Che,Feng Ye,Zhaoqiang Zhang,Peitao Xu,Yipeng Zeng,Xiaojun Xu,Zhikun Li,Pei Feng,Rujia Mi,Yanfeng Wu,Huiyong ShenPubMed 37280207
- International journal of molecular sciences 24:2023Nuclear DJ-1 Regulates DNA Damage Repair via the Regulation of PARP1 Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhong-Xuan Wang,Yi Liu,Yao-Lin Li,Qiao Wei,Rong-Rong Lin,Ruiqing Kang,Yang Ruan,Zhi-Hao Lin,Nai-Jia Xue,Bao-Rong Zhang,Jia-Li PuPubMed 37239999
- Birth defects research 115:770-7812023Effects of ketamine-induced H3K9 hypoacetylation during pregnancy on cardiogenesis of mouse offspring.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYujuan Yu,Junjun Quan,Mou Zou,Wei Zhao,Yujuan Su,Ying XuPubMed 36899481
- Journal of cellular physiology 238:992-10052023Obesity-mediated upregulation of the YAP/IL33 signaling axis promotes aggressiveness and induces an immunosuppressive tumor microenvironment in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia-Zih Dai,Ching-Chieh Yang,Pei-Wei Shueng,Yen-Ju Wang,Chen-Shiuan Huang,Yi-Chun Chao,Cheng-Hsun Chen,Cheng-Wei LinPubMed 36852589
- Journal of nanobiotechnology 21:372023Apoptotic tumor cell-derived microparticles loading Napabucasin inhibit CSCs and synergistic immune therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBoping Jing,Feng Guo,Rui An,Yu Gao,Yuman Li,Yuji Xie,Jing Wang,Yihan Chen,He Li,Tang Gao,Qiaofeng Jin,Li Zhang,Mingxing XiePubMed 36732759
- Cell death & disease 14:472023TRIM15 forms a regulatory loop with the AKT/FOXO1 axis and LASP1 to modulate the sensitivity of HCC cells to TKIs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChong Yang,Xin Jin,Xingchao Liu,Gang Wu,Wenhao Yang,Beichuan Pang,Jipeng Jiang,Dongxu Liao,Yu ZhangPubMed 36670097
Key facts
- Assay time
- 1h
- Sample type
- Tissue, Suspension cells, Adherent cells
What's included?
Recommended products
60-minute chromatin extraction kit is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells and tissues in a simple and rapid format.
- Cited in over 50 publications
Key facts
- Assay time
- 1h
- Sample type
- Tissue, Suspension cells, Adherent cells
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
60-minute chromatin extraction kit is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells and tissues in a simple and rapid format.
Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Functional Studies - Chromatin Extraction Kit (ab117152)
ChIP analysis of RNA polymerase II enriched in GAPDH and MLH1 promoters with chromatin extract prepared from formaldehyde fixed colon cancer cells (2x105) using ab117152.
Functional Studies - Chromatin Extraction Kit (ab117152)
Chromatin was extracted from 3x106 mouse neuroblastoma cells, fixed for 10min at RT in 0.1% formaldehyde. Cells were sonicated before last centrifugation, and following centrifugation, cells were treated with proteinase K & RNAse A.
Chromatin yield was 4 μg chromatin/106 cells.
Lane 1: 1kb DNA ladder
Lane 2: 300 - 650bp DNA fragments
Image courtesy of anonymous Abreview
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com