60-minute chromatin extraction kit is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells and tissues in a simple and rapid format.
- Cited in over 50 publications
- FuncS
Supplier Data
Functional Studies - Chromatin Extraction Kit (AB117152)
ChIP analysis of RNA polymerase II enriched in GAPDH and MLH1 promoters with chromatin extract prepared from formaldehyde fixed colon cancer cells (2x105) using ab117152.
- FuncS
Unknown
Functional Studies - Chromatin Extraction Kit (AB117152)
Chromatin was extracted from 3x106 mouse neuroblastoma cells, fixed for 10min at RT in 0.1% formaldehyde. Cells were sonicated before last centrifugation, and following centrifugation, cells were treated with proteinase K & RNAse A.
Chromatin yield was 4 μg chromatin/106 cells.
Lane 1 : 1kb DNA ladder
Lane 2 : 300 - 650bp DNA fragments
Image courtesy of anonymous Abreview
Product details
60-minute chromatin extraction kit is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells and tissues in a simple and rapid format.
Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (51)
Recent publications for all applications. Explore the full list and refine your search
Cancer biology & therapy 25:2329372 PubMed38494680
2024
Applications
Unspecified application
Species
Unspecified reactive species
Nature communications 15:1579 PubMed38383676
2024
Applications
Unspecified application
Species
Unspecified reactive species
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2302368 PubMed37949681
2023
Applications
Unspecified application
Species
Unspecified reactive species
eLife 12: PubMed37603466
2023
Applications
Unspecified application
Species
Unspecified reactive species
Bone research 11:30 PubMed37280207
2023
Applications
Unspecified application
Species
Unspecified reactive species
International journal of molecular sciences 24: PubMed37239999
2023
Applications
Unspecified application
Species
Unspecified reactive species
Birth defects research 115:770-781 PubMed36899481
2023
Applications
Unspecified application
Species
Unspecified reactive species
Journal of cellular physiology 238:992-1005 PubMed36852589
2023
Applications
Unspecified application
Species
Unspecified reactive species
Journal of nanobiotechnology 21:37 PubMed36732759
2023
Applications
Unspecified application
Species
Unspecified reactive species
Cell death & disease 14:47 PubMed36670097
2023
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com