AB117152

Chromatin Extraction Kit

Name: Chromatin extraction kit - 100 tests (ab117152) | Abcam
Brand: Abcam Limited
SKU: AB117152

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(75 Publications)

Chromatin Extraction Kit (ab117152) offers a simple and rapid 60-minute format to extract chromatin with a complete set of optimized buffers and reagents. Isolates native chromatin or DNA-protein complex from mammalian cells and tissues samples.

- Chromatin prepared by this kit compatible for various chromatin immunoprecipitation (ChIP) assays.
- Suitable for in vitro protein-DNA binding assays and nuclear enzyme assays
- Proven performance: cited in over 50 publications.

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Functional Studies - Chromatin Extraction Kit (AB117152)

FuncS

Supplier Data

Functional Studies - Chromatin Extraction Kit (AB117152)

ChIP analysis of RNA polymerase II enriched in GAPDH and MLH1 promoters with chromatin extract prepared from formaldehyde fixed colon cancer cells (2x10⁵) using ab117152.

FuncS

Unknown

Functional Studies - Chromatin Extraction Kit (AB117152)

Chromatin was extracted from 3x10⁶ mouse neuroblastoma cells, fixed for 10min at RT in 0.1% formaldehyde. Cells were sonicated before last centrifugation, and following centrifugation, cells were treated with proteinase K & RNAse A.
Chromatin yield was 4 μg chromatin/10⁶ cells.

Lane 1 : 1kb DNA ladder
Lane 2 : 300 - 650bp DNA fragments

Image courtesy of anonymous Abreview

Key facts

Assay time

Sample types

Tissue, Suspension cells, Adherent cells

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Product details

60-minute chromatin extraction kit is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells and tissues in a simple and rapid format.

Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab117152-100Test", "assetComponentDetails": [ { "size":"1 x 110 µL", "name":"1000X Protease Inhibitor Cocktail", "number":"AB117152-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Extraction Buffer", "number":"AB117152-CMP03", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromatin Buffer", "number":"AB117152-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"10X Lysis Buffer", "number":"AB117152-CMP01", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Product protocols

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Target data

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Publications (75)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed40553569

2025

MUC1-C dependence in treatment-resistant prostate cancer uncovers a target for antibody-drug conjugate therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Keisuke Shigeta,Tatsuaki Daimon,Hiroshi Hongo,Sheng-Yu Ku,Hiroki Ozawa,Naoki Haratake,Atsushi Fushimi,Ayako Nakashoji,Atrayee Bhattacharya,Shinkichi Takamori,Michihisa Kono,Masahiro Rokugo,Yuto Baba,Takeo Kosaka,Mototsugu Oya,Justine Jacobi,Mark D Long,Himisha Beltran,Donald Kufe

eLife 14: PubMed40464376

2025

Targeting SLC7A11-mediated cysteine metabolism for the treatment of trastuzumab-resistant HER2-positive breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yijia Hua,Ningjun Duan,Chunxiao Sun,Fan Yang,Min Tian,Yanting Sun,Shuhan Zhao,Jue Gong,Qian Liu,Xiang Huang,Yan Liang,Ziyi Fu,Wei Li,Yongmei Yin

NPJ precision oncology 9:78 PubMed40113969

2025

Multi-omics analysis identifies glioblastoma dependency on H3K9me3 methyltransferase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Qiqi Xie,Yuanning Du,Sugata Ghosh,Saranya Rajendran,Aaron A Cohen-Gadol,José-Manuel Baizabal,Kenneth P Nephew,Leng Han,Jia Shen

Cell reports. Medicine 6:102000 PubMed40056904

2025

LTA4H improves the tumor microenvironment and prevents HCC progression via targeting the HNRNPA1/LTBP1/TGF-β axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Yang,Xinyao Qiu,Yingcheng Yang,Jing Wu,Shan Wang,Bo Zheng,Jianmin Wu,Tao Zhou,Yangqianwen Zhang,Mixue Bai,Shuowu Liu,Zihan Zhao,Yani Zhang,Yixian Wang,Jinxia Bao,Mengye Wu,Dongdong Xue,Meiyu Bao,Ji Hu,Siyun Shen,Hongyang Wang,Lei Chen

Experimental & molecular medicine 57:264-280 PubMed39870799

2025

RECQL4 requires PARP1 for recruitment to DNA damage, and PARG dePARylation facilitates its associated role in end joining.

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Unspecified application

Species

Unspecified reactive species

Mansoor Hussain,Prabhat Khadka,Komal Pekhale,Tomasz Kulikowicz,Samuel Gray,Alfred May,Deborah L Croteau,Vilhelm A Bohr

iScience 28:111614 PubMed39834855

2025

Downregulation of ECRG4 by DNMT1 promotes EC growth via IRF3/IFN-γ/miR-29b/DNMT1/ECRG4 positive feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Yang,Shuaining Chai,Helong Song,Sinan Cao,Fangmiao Gao,Chenxuan Zhou,Linwei Li

Science advances 10:eadq4274 PubMed39661665

2024

SRC kinase drives multidrug resistance induced by KRAS-G12C inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Xinxin Song,Zhuan Zhou,Ammar Elmezayen,Runliu Wu,Chunhua Yu,Boning Gao,John D Minna,Kenneth D Westover,Herbert J Zeh,Guido Kroemer,Lynn E Heasley,Rui Kang,Daolin Tang

Advanced biology 9:e2400413 PubMed39601498

2024

CBX3 Downregulates HLTF to Activate PI3K/AKT Signaling Promoting Cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Min Xie,Huaiyuan Liang,Yuxuan Mao,Yuping Yao,Bingzhang Tian

Nature communications 15:9463 PubMed39487138

2024

Increased DNA damage in full-grown oocytes is correlated with diminished autophagy activation.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Sun,Nourhan Nashat Ali,Daniela Londoño-Vásquez,Constantine A Simintiras,Huanyu Qiao,M Sofia Ortega,Yuksel Agca,Masashi Takahashi,Rocío M Rivera,Andrew M Kelleher,Peter Sutovsky,Amanda L Patterson,Ahmed Z Balboula

Cell reports. Medicine 5:101806 PubMed39481387

2024

Lnc-H19-derived protein shapes the immunosuppressive microenvironment of glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Junju Chen,Yixin Gao,Jian Zhong,Xujia Wu,Zhaojie Leng,Ming Liu,Yesheng Wang,Yuan Wang,Xuesong Yang,Nunu Huang,Feizhe Xiao,Maolei Zhang,Xuesong Liu,Nu Zhang

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Chromatin Extraction Kit

Functional Studies - Chromatin Extraction Kit (AB117152)

Functional Studies - Chromatin Extraction Kit (AB117152)

Key facts

Assay time

Sample types

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (75)

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