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AB223876

Chromatin Extraction Kit - Flexible Format

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Chromatin Extraction Kit (ab223876) is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format.
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ChIP - Chromatin Extraction Kit - Flexible Format (AB223876)
  • ChIP

Lab

ChIP - Chromatin Extraction Kit - Flexible Format (AB223876)

Analysis of chromatin fragmentation following 15min of sonication.

Chromatin was prepared from various amount of cross-linked HeLa cells (human cervical cancer) resuspended in 0.5 mL of extraction and lysis buffer. Left to right : 10×106, 5×106, 2×106, 1×106, 1×105 cells per prep.

ChIP - Chromatin Extraction Kit - Flexible Format (AB223876)
  • ChIP

Lab

ChIP - Chromatin Extraction Kit - Flexible Format (AB223876)

Analysis of chromatin fragmentation following 15min of sonication.

Chromatin was prepared from 10×106 of cross-linked HeLa cells (human cervical cancer) using ab223876 (left lane) or competitor's kit (right lane) as per corresponding manuals. Chromatin fragmentation was analysed using standard method. For ChIP experiments chromatin must be sheared to fragments < 1000 bp.

ChIP - Chromatin Extraction Kit - Flexible Format (AB223876)
  • ChIP

Lab

ChIP - Chromatin Extraction Kit - Flexible Format (AB223876)

Analysis of chromatin fragmentation following 15min of sonication.

Chromatin was prepared from 10×106 cells of cross-linked cells or 30 mg of tissue using extraction and lysis buffer. Left to right : Mw markers, HeLa (human cervical cancer), A549 (human lung carcinoma), F9 (mouse 129 embryo teratocarcinoma), mouse liver.

Key facts

Sample types

Tissue, Suspension cells, Adherent cells

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Product details

Chromatin Extraction Kit (ab223876) is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab223876-100Test", "assetComponentDetails": [ { "size":"1 x 0.5 mL", "name":"Protease Inhibitor Cocktail", "number":"AB223876-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"Stringency supplement", "number":"AB223876-CMP05", "productcode":"" }, { "size":"1 x 100 mL", "name":"Extraction and Lysis Buffer", "number":"AB223876-CMP02", "productcode":"" }, { "size":"1 x 5 mL", "name":"IP Buffer Concentrate (11X)", "number":"AB223876-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Formaldehyde Quenching Solution (10X)", "number":"AB223876-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Please refer to protocols
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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