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AB185913

High-Sensitivity ChIP Kit

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(44 Publications)

High-Sensitivity ChIP kit (ab185913) is a complete set of optimized reagents to carry out a successful chromatin immunoprecipitation (ChIP) procedure in a high-throughput format starting from mammalian cells or tissues in 5 hours. Both negative and positive ChIP controls are provided in this kit.

- Cited in over 40 publications
- Optimized to reduce non-specific background signal and detects low-abundance targets
- Highly efficient enrichment ratio of positive to negative control is > 500
- Compatible with ChIP-PCR, microarrays & sequencing (ChIP-seq)
3 Images
ChIP - High-Sensitivity ChIP Kit (AB185913)
  • ChIP

Supplier Data

ChIP - High-Sensitivity ChIP Kit (AB185913)

High abundance protein enrichment : .

Sheared chromatin isolated from different numbers of MBD-231 cells was used for ChIP-qPCR analysis of RNA polymerase II enrichment in GAPDH promoters using ab185913 and a quantitative PCR Fast Kit.

ChIP - High-Sensitivity ChIP Kit (AB185913)
  • ChIP

Supplier Data

ChIP - High-Sensitivity ChIP Kit (AB185913)

Low abundance protein enrichment : .

Sheared chromatin isolated from different numbers of MCF7 cells was used for ChIP-qPCR analysis of ER-a enrichment in TFF1 promoters using ab185913 and a quantitative PCR Fast Kit.

ChIP-sequencing - High-Sensitivity ChIP Kit (AB185913)
  • ChIP-seq

Supplier Data

ChIP-sequencing - High-Sensitivity ChIP Kit (AB185913)

Histone H3K18ac ChIP assay example data.

Histone H3K18ac ChIP assay was carried out using ab185913.

ChIP-Seq reads align with the same peak sites as ENCODE data.

Key facts

Sample types

Adherent cells, Suspension cells, Tissue

Reacts with

Mammals

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ChIP": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>The GAPDH primers provided with the kit are for the human sequence. If using the kit with a different species, GAPDH primers for that species will need to be acquired.</p>" } } }
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Product details

High-Sensitivity ChIP Kit (ab185913) is a complete set of optimized reagents to carry out a successful chromatin immunoprecipitation procedure in a high throughput format starting from mammalian cells or tissues. The highly specific and sensitive kit is suitable for selective enrichment of a chromatin fraction containing specific DNA sequences using various mammalian cell/tissues. The optimized protocol and kit components reduce non-specific background ChIP levels to allow capture of low abundance protein/transcription factors and increased specific enrichment of target protein/DNA complexes. The target protein bound DNA prepared with the High-Sensitivity ChIP Kit can be used for various downstream applications including PCR (ChIP-PCR), microarrays (ChIP-on-chip), and sequencing (ChIP-seq).

Starting Materials
Starting materials can include various tissue or cell samples such as cells from flask or plate cultured cells, fresh and frozen tissues, etc. In general, the amount of cells and tissues for each reaction can be 2 x 103 to 1 x 106 and 0.5 mg to 50 mg, respectively. For optimal preparation, the input amount should be 1-2 x 105 cells or 10-20 mg tissues since the enrichment of target proteins to genome loci may vary. For the target proteins that are low abundance transcription factors, the input amount should be 5-6 x 105 cells or 50 to 60 mg tissues.

Primers
The GAPDH primers provided with the kit are for the human sequence. If using the kit with a difference species, GAPDH primers for that species will need to be acquired.

Protein-DNA interaction plays a critical role for cellular functions such as signal transduction, gene transcription, chromosome segregation, DNA replication and recombination, and epigenetic silencing. Identifying the genetic targets of DNA binding proteins and knowing the mechanisms of protein-DNA interaction is important for understanding cellular processes. Chromatin immunoprecipitation (ChIP) offers an advantageous tool for studying such protein-DNA interactions. It allows for the detection of a specific protein bound to a specific gene sequence in living cells using PCR (ChIP-PCR), microarrays (ChIP-chip), or sequencing (ChIP-seq). For example, measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated with a specific gene promoter region under various conditions can be achieved through a ChIP-PCR assay, while the recruitment of methylated H3-K9 to the promoters on a genome-wide scale can be detected by ChIP-on-chip or ChIP-sequencing. ChIP analysis requires that ChIPed DNA contains minimal background in order to reliably identify true TF-enriched regions. High background in ChIP is mainly caused ineffective wash buffers, insufficient cross-link reversal, inappropriate DNA fragment length, and residual RNA interference. To effectively capture TF/DNA complexes, which are often in low abundance, an ideal ChIP method requires having maximum sensitivity with minimized background levels. This method should also be able to enrich highly abundant protein/DNA complexes using a small amount of cells or tissues in a high throughput format. The High-Sensitivity ChIP Kit is designed to achieve these goals by maximizing sensitivity and minimizing non-specific background signals. **ChIP assay products and guides** Find more resources and products, products, and other .

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (44)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in aging neuroscience 15:1326127 PubMed38192280

2024

O-GlcNAc regulates the mitochondrial integrated stress response by regulating ATF4.

Applications

Unspecified application

Species

Unspecified reactive species

Ibtihal M Alghusen,Marisa S Carman,Heather Wilkins,Sophiya John Ephrame,Amy Qiang,Wagner B Dias,Halyna Fedosyuk,Aspin R Denson,Russell H Swerdlow,Chad Slawson

Cell death & disease 14:767 PubMed38007473

2023

LINC01133 can induce acquired ferroptosis resistance by enhancing the FSP1 mRNA stability through forming the LINC01133-FUS-FSP1 complex.

Applications

Unspecified application

Species

Unspecified reactive species

Shaowen Wang,Jionghuang Chen,Pengping Li,Yangchao Chen

Cellular & molecular biology letters 28:93 PubMed37993768

2023

Disrupted cardiac fibroblast BCAA catabolism contributes to diabetic cardiomyopathy via a periostin/NAP1L2/SIRT3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qing-Bo Lu,Xiao Fu,Yao Liu,Zi-Chao Wang,Shi-Yi Liu,Yu-Chao Li,Hai-Jian Sun

iScience 26:108030 PubMed37920670

2023

Loss of histone reader Phf7 leads to immune pathways activation via endogenous retroviruses during spermiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jianxing Cheng,Tongtong Li,Zhongjie Zheng,Xueguang Zhang,Mengyang Cao,Wenhao Tang,Kai Hong,Rui Zheng,Jichun Shao,Xiaomiao Zhao,Hui Jiang,Wenming Xu,Haocheng Lin

Biomarker research 11:74 PubMed37553583

2023

Identification of GRIN2D as a novel therapeutic target in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiatong Wang,Chi Hin Wong,Yinxin Zhu,Xiaoqiang Yao,Kelvin K C Ng,Chengzhi Zhou,Ka Fai To,Yangchao Chen

Cells 12: PubMed37174649

2023

Postmortem Brains from Subjects with Diabetes Mellitus Display Reduced GLUT4 Expression and Soma Area in Hippocampal Neurons: Potential Involvement of Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Caio Yogi Yonamine,Marisa Passarelli,Claudia Kimie Suemoto,Carlos Augusto Pasqualucci,Wilson Jacob-Filho,Venâncio Avancini Ferreira Alves,Suely Kazue Nagahashi Marie,Maria Lucia Correa-Giannella,Luiz Roberto Britto,Ubiratan Fabres Machado

Cell death discovery 9:106 PubMed36977670

2023

Caspase 6/NR4A1/SOX9 signaling axis regulates hepatic inflammation and pyroptosis in ischemia-stressed fatty liver.

Applications

Unspecified application

Species

Unspecified reactive species

Mingwei Sheng,Yiqi Weng,Yingli Cao,Chen Zhang,Yuanbang Lin,Wenli Yu

Nature communications 14:1214 PubMed36869048

2023

mTORC1 upregulates B7-H3/CD276 to inhibit antitumor T cells and drive tumor immune evasion.

Applications

Unspecified application

Species

Unspecified reactive species

Heng-Jia Liu,Heng Du,Damir Khabibullin,Mahsa Zarei,Kevin Wei,Gordon J Freeman,David J Kwiatkowski,Elizabeth P Henske

JCI insight 8: PubMed36656644

2023

Hypoxia enhances IPF mesenchymal progenitor cell fibrogenicity via the lactate/GPR81/HIF1α pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Libang Yang,Adam Gilbertsen,Hong Xia,Alexey Benyumov,Karen Smith,Jeremy Herrera,Emil Racila,Peter B Bitterman,Craig A Henke

Frontiers in bioscience (Landmark edition) 28:17 PubMed36722273

2023

Regulates the Signal Axis through Epigenetic Modification to Promote the Migration and Invasion of Papillary Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Wang,Yibo He,Yi Wang,Shangnao Xie
View all publications
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