Histone Extraction Kit - Rapid / Ultra-pure

ab221031 Histone Extraction Kit - Rapid / Ultra-pure enables the extraction of core histones from mammalian cells or tissues in 1 hour using a simple procedure with 20 minutes hands-on time. As post-translational modifications, such as acetylation, methylation and phosphorylation are maintained, histones extracted using this kit can be used in a wide variety of downstream applications.

This kit is compatible with three distinct protocols: Standard, Rapid and Ultra-pure Histone Extraction. The Standard Extraction protocol is a 1 hour procedure (of which 20 minutes is hands-on) that is suitable for histone extraction from a wide range of cell lines and tissues. The Rapid Extraction protocol can be used to prepare a crude histone extract quickly for use in applications where histone purity is not critical. It is also recommended for histone extraction using fragile cell lines or in instances where extraction of cytoplasmic histones is desirable. This protocol takes 45 minutes and requires 15 minutes of hands-on time. The Ultra-pure Histone Extraction protocol is a 75-minute procedure (with 25 minutes hands-on time) that yields very pure histones from a wide range of cell lines and tissues.

Each kit contains reagents to perform 100 extractions, where each extraction requires 2 x 10⁶ cells or 20 mg of tissue starting material. Typical yields are around 80 μg of histones from 2 x 10⁶ HeLa cells.

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