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DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC).

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Assay time

3h

Reactive species

Mouse, Human

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What's included?

96 Test
Components
10X Wash Buffer
1 x 20 mL
5-hmC Antibody, 0.6 mg/mL
1 x 100 µL
8-Well Assay Strips (with Frame)
12 x 1 Unit
Adhesive 8-Well Strip Film
12 x 1 Unit
Antibody Buffer
1 x 16 mL
Control DNA, 500 ng/mL
1 x 20 µL
Control Primer-Forward, 20 µM
1 x 20 µL
Control Primer-Reverse, 20 µM
1 x 20 µL
DNA Release Buffer
1 x 28 mL
Non-Immune IgG ,0.6 mg/mL
1 x 40 µL
Proteinase K, 10 mg/mL
1 x 112 µL
hMeDIP Solution
1 x 12 mL

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DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC).

Key facts

Assay time

3h

Reactive species

Mouse, Human

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide, 5-hydroxymethylcytosine (5-hmC), has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation.

Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit (ab117134) enables the user to enrich hydroxymethated DNA using an antibody specific to hydroxymethylcytosine (5-hmC) to immunoprecipitate hydroxymethylated genomic DNA hMeDIP. The enriched hydroxymethylated fractions can be used in various downstream applications including PCR (hMeDIP-PCR) and microarrays (hMeDIP-chip) for investigating gene-specific hydroxymethylation in cells or tissues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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