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AB117134

Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit

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(4 Publications)

Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit (ab117134) contains reagents required for hMeDIP procedure for the selective enrichment of DNA fragments containing 5-hydroxymethylcytosine (5-hmC) from mammalian cells or tissues.

- Includes a positive control DNA fragement and negative control (Non-immune IgG)
- Highly efficient enrichment ratio of positive/negative control > 1000
- Compatible with various downstream applications including PCR (hMeDIP-PCR) and microarray (hMeDIP-chip)
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Functional Studies - Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit (AB117134)
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Supplier Data

Functional Studies - Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit (AB117134)

Sensitive detection of gene-specific hydroxymethylation by hMeDIP-QPCR.

Human brain DNA (500 ng) was fragmented to 200-600 bps with a sonicator. The fragmented DNA was used for hydroxymethylated DNA enrichment with ab117134. Eluted DNA was analyzed by real time PCR with primers specifically for OCT4 or GAPDH sequences in the promoter regions. Results show that the promoter region is hydroxymethylated in OCT4 but not in GAPDH.

Functional Studies - Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit (AB117134)
  • FuncS

Supplier Data

Functional Studies - Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit (AB117134)

Selective enrichment of hydroxymethyated DNA with ab117134.

50 pg of unmethylated, methylated, and hydroxymethylated DNA control were spiked into fragmented human genomic DNA (500 ng). hMeDIP was processed using 5-hmC antibody and non-immune IgG included in the kit. Eluted DNA was analyzed by real time PCR using control primers included in the kit to detect the presence of spiked control DNA.

Key facts

Assay time

3h

Reacts with

Mouse, Human

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Sample Prep & Detection Kits

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Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA

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Primary Antibodies

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Product details

DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide, 5-hydroxymethylcytosine (5-hmC), has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation.

Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit (ab117134) enables the user to enrich hydroxymethated DNA using an antibody specific to hydroxymethylcytosine (5-hmC) to immunoprecipitate hydroxymethylated genomic DNA hMeDIP. The enriched hydroxymethylated fractions can be used in various downstream applications including PCR (hMeDIP-PCR) and microarrays (hMeDIP-chip) for investigating gene-specific hydroxymethylation in cells or tissues.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 13:e1518 PubMed38148658

2023

Gender dimorphism in hepatocarcinogenesis-DNA methylation modification regulated X-chromosome inactivation escape molecule XIST.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihui Dai,Sijie Wang,Xinggang Guo,Yuefan Wang,Haozan Yin,Jian Tan,Chenyang Mu,Shu-Han Sun,Hui Liu,Fu Yang

iScience 24:103065 PubMed34568789

2021

Loss of miR-29a impairs decidualization of endometrial stromal cells by TET3 mediated demethylation of Col1A1 promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Aixia Liu,Mengmeng Jin,Laidi Xie,Mengyu Jing,Ying Zhou,Minyue Tang,Tingting Lin,Dimin Wang

PLoS genetics 16:e1008823 PubMed32453789

2020

Glucose transporter 10 modulates adipogenesis via an ascorbic acid-mediated pathway to protect mice against diet-induced metabolic dysregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Chung-Lin Jiang,Wei-Ping Jen,Chang-Yu Tsao,Li-Ching Chang,Chien-Hsiun Chen,Yi-Ching Lee

PloS one 9:e107905 PubMed25229454

2014

Epigenetic mechanisms underlying the dynamic expression of cancer-testis genes, PAGE2, -2B and SPANX-B, during mesenchymal-to-epithelial transition.

Applications

FuncS

Species

Human

Sinem Yilmaz-Ozcan,Asli Sade,Baris Kucukkaraduman,Yasemin Kaygusuz,Kerem Mert Senses,Sreeparna Banerjee,Ali Osmay Gure
View all publications
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