DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC).
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- Clinical and translational medicine 13:e15182023Gender dimorphism in hepatocarcinogenesis-DNA methylation modification regulated X-chromosome inactivation escape molecule XIST.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhihui Dai,Sijie Wang,Xinggang Guo,Yuefan Wang,Haozan Yin,Jian Tan,Chenyang Mu,Shu-Han Sun,Hui Liu,Fu YangPubMed 38148658
- iScience 24:1030652021Loss of miR-29a impairs decidualization of endometrial stromal cells by TET3 mediated demethylation of Col1A1 promoter.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAixia Liu,Mengmeng Jin,Laidi Xie,Mengyu Jing,Ying Zhou,Minyue Tang,Tingting Lin,Dimin WangPubMed 34568789
- PLoS genetics 16:e10088232020Glucose transporter 10 modulates adipogenesis via an ascorbic acid-mediated pathway to protect mice against diet-induced metabolic dysregulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChung-Lin Jiang et. al.PubMed 32453789
- PloS one 9:e1079052014Epigenetic mechanisms underlying the dynamic expression of cancer-testis genes, PAGE2, -2B and SPANX-B, during mesenchymal-to-epithelial transition.
Key facts
- Assay time
- 3h
- Reactive species
- Mouse, Human
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DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC).
Key facts
- Assay time
- 3h
- Reactive species
- Mouse, Human
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide, 5-hydroxymethylcytosine (5-hmC), has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation.
Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit (ab117134) enables the user to enrich hydroxymethated DNA using an antibody specific to hydroxymethylcytosine (5-hmC) to immunoprecipitate hydroxymethylated genomic DNA hMeDIP. The enriched hydroxymethylated fractions can be used in various downstream applications including PCR (hMeDIP-PCR) and microarrays (hMeDIP-chip) for investigating gene-specific hydroxymethylation in cells or tissues.
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Functional Studies - Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit (ab117134)
Sensitive detection of gene-specific hydroxymethylation by hMeDIP-QPCR.
Human brain DNA (500 ng) was fragmented to 200-600 bps with a sonicator. The fragmented DNA was used for hydroxymethylated DNA enrichment with ab117134. Eluted DNA was analyzed by real time PCR with primers specifically for OCT4 or GAPDH sequences in the promoter regions. Results show that the promoter region is hydroxymethylated in OCT4 but not in GAPDH.
Functional Studies - Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit (ab117134)
Selective enrichment of hydroxymethyated DNA with ab117134.
50 pg of unmethylated, methylated, and hydroxymethylated DNA control were spiked into fragmented human genomic DNA (500 ng). hMeDIP was processed using 5-hmC antibody and non-immune IgG included in the kit. Eluted DNA was analyzed by real time PCR using control primers included in the kit to detect the presence of spiked control DNA.
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