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Membrane Fluidity Assay Kit ab189819 uses a fluorescent lipophilic pyrene probe that forms excimers on spatial interaction, this shifts its emission from Em 372 nm to Em 470 nm. Measuring the ratio of 372 nm to 470 nm fluorescence gives a quantitative measure of membrane fluidity.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

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Detection method

Fluorescent

Assay Platform

Flow cytometer, Fluorescence microscope

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Images

What's included?

100 Test
Components
Fluorescent Lipid Reagent
1 x 2 mL
Perfusion Buffer
1 x 25 mL
Pluronic F-127
1 x 50 mg
Reference Standard
1 x 2 mL

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Membrane Fluidity Assay Kit ab189819 uses a fluorescent lipophilic pyrene probe that forms excimers on spatial interaction, this shifts its emission from Em 372 nm to Em 470 nm. Measuring the ratio of 372 nm to 470 nm fluorescence gives a quantitative measure of membrane fluidity.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Fluorescent

Assay Platform

Flow cytometer, Fluorescence microscope

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Membrane fluidity or "membrane viscosity" for short range lateral diffusion has best been measured using lipid analog probes that, when interacting, exhibit changes in their spectral properties. One of the best systems for use in such studies are the lipophilic pyrene probes that undergo excimer formation upon spatial interaction. When excimers form, the emission spectrum of the pyrene probe shifts dramatically to the red (longer wavelength). By measuring the ratio of monomer (EM max. 372 nm) to excimer (EM 470 nm) fluorescence, a quantitative monitoring of the membrane fluidity can be attained. These measurements can provide kinetic information, as well as in vivo monitoring of cellular function by both flow cytometry and microscopic analysis.

Exitation wavelength (nm): 350

Emission wavelength (nm): 450

The dynamic properties of the cell membrane and cytoplasmic microtubules and microfilaments, as well as the dynamic movement of lipids in micelles and vesicles is of importance in such diverse areas as activation of polymorphonuclear leukocytes and chemotaxis, activation of membrane enzyme systems and the specific assembly or mobilization of microtubules and microfilaments, enhancement of the affinity of chemoattractant receptors, as well as being associated with a variety of pathological syndromes related to membrane fluidity.

It has been recognized that the rotational mobility of fluorescent or magnetic resonant probes is different from that observed in lateral diffusion.

Kit size: 100 tests in a 50 μL reaction volume.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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