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AB117135

Methylated DNA Immunoprecipitation (MeDIP) ChIP Kit

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(6 Publications)

Methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117135) contains reagents required for carrying out methylated DNA immunoprecipitation directly from mammalian cells.

- Includes a positive control antibody (5-methylcytosine) and negative control (Non-immune IgG)
- Highly efficient enrichment of methylated DNA: >95%
- Compatible with various downstream applications including qPCR, MS-PCR, DNA sequencing, Southern blot and DNA microarray.

Key facts

Assay time

4h

Sample types

Suspension cells, Adherent cells

Reacts with

Mouse, Human

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Product details

DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.

Methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117135) enables the user to enrich methylated DNA directly from cells by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Genes & diseases 12:101519 PubMed40612667

2025

Restriction of YWHAB-mediated YAP cytoplasmic retention is a novel mechanism underlying stemness maintenance and chemoresistance in ovarian cancer peritoneal metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Liu,Lei Shi,Zijun Meng,Manlin Zhang,Zhiqi Zhang,Yunzhe Li,Kaiwen Du,Muyao Yang,Lin Qiu,Jing Feng,Yuchen He,Jiayun Liu,Hua Zhang,Hongbin Zhang,Tingyuan Lang,Zhuo Yang

eLife 14: PubMed40464376

2025

Targeting SLC7A11-mediated cysteine metabolism for the treatment of trastuzumab-resistant HER2-positive breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yijia Hua,Ningjun Duan,Chunxiao Sun,Fan Yang,Min Tian,Yanting Sun,Shuhan Zhao,Jue Gong,Qian Liu,Xiang Huang,Yan Liang,Ziyi Fu,Wei Li,Yongmei Yin

Nature communications 15:7522 PubMed39215042

2024

SLC13A3 is a major effector downstream of activated β-catenin in liver cancer pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Wennan Zhao,Xue Wang,Lifeng Han,Chunze Zhang,Chenxi Wang,Dexin Kong,Mingzhe Zhang,Tong Xu,Gen Li,Ge Hu,Jiahua Luo,Sook Wah Yee,Jia Yang,Andreas Stahl,Xin Chen,Youcai Zhang

Clinical and translational medicine 13:e1518 PubMed38148658

2023

Gender dimorphism in hepatocarcinogenesis-DNA methylation modification regulated X-chromosome inactivation escape molecule XIST.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihui Dai,Sijie Wang,Xinggang Guo,Yuefan Wang,Haozan Yin,Jian Tan,Chenyang Mu,Shu-Han Sun,Hui Liu,Fu Yang

Molecular medicine (Cambridge, Mass.) 26:50 PubMed32450789

2020

Down-regulated miR-495 can target programmed cell death 10 in ankylosing spondylitis.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Juan Ni,Xiao-Min Leng

Biochimica et biophysica acta. Molecular basis of disease 1864:3298-3308 PubMed30053437

2018

The von Hippel Lindau tumour suppressor gene is a novel target of E2F4-mediated transcriptional repression in preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Sruthi Alahari,Julia Garcia,Martin Post,Isabella Caniggia
View all publications
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