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DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes.

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Key facts

Assay time
4h
Sample type
Suspension cells, Adherent cells
Reactive species
Mouse, Human

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What's included?

48 Test
Components
8-Well Assay Strips (with Frame)
6 x 1 Unit
8-Well Strip Caps
6 x 1 Unit
Anti-5-Methylcytosine (1 mg/mL)
1 x 50 µL
Antibody Buffer
1 x 30 mL
Binding Buffer
1 x 8 mL
ChIP Dilution Buffer
1 x 6 mL
DNA Release Buffer
2 x 2 mL
Elution Buffer
1 x 1.2 mL
F-Collection Tube
50 x 1 Unit
F-Spin Column
50 x 1 Unit
Lysis Buffer
1 x 6 mL
Normal Mouse IgG (1 mg/mL)
1 x 15 µL
Pre-Lysis Buffer
1 x 8 mL
Proteinase K (10 mg/mL)
1 x 50 µL
Reverse Buffer
2 x 2 mL
Wash Buffer
2 x 28 mL

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DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes.

Key facts

Assay time
4h
Sample type
Suspension cells, Adherent cells
Reactive species
Mouse, Human

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.

Methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117135) enables the user to enrich methylated DNA directly from cells by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.

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