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DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes.

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Assay time

4h

Sample type

Suspension cells, Adherent cells

Reactive species

Mouse, Human

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What's included?

48 Test
Components
8-Well Assay Strips (with Frame)
6 x 1 Unit
8-Well Strip Caps
6 x 1 Unit
Anti-5-Methylcytosine (1 mg/mL)
1 x 50 µL
Antibody Buffer
1 x 30 mL
Binding Buffer
1 x 8 mL
ChIP Dilution Buffer
1 x 6 mL
DNA Release Buffer
2 x 2 mL
Elution Buffer
1 x 1.2 mL
F-Collection Tube
50 x 1 Unit
F-Spin Column
50 x 1 Unit
Lysis Buffer
1 x 6 mL
Normal Mouse IgG (1 mg/mL)
1 x 15 µL
Pre-Lysis Buffer
1 x 8 mL
Proteinase K (10 mg/mL)
1 x 50 µL
Reverse Buffer
2 x 2 mL
Wash Buffer
2 x 28 mL

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DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes.

Key facts

Assay time

4h

Sample type

Suspension cells, Adherent cells

Reactive species

Mouse, Human

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.

Methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117135) enables the user to enrich methylated DNA directly from cells by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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