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AB117133

Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA

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(12 Publications)

Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (ab117133) contains reagents required to enrich methylated DNA from a DNA sample by isolating methylated DNA fragments with a 5-methylcytosine antibody.

- Includes a positive control antibody (5-methylcytosine) and negative control (Non-immune IgG)
- Highly efficient enrichment of methylated DNA: >98%
- Compatible with various downstream applications including qPCR, DNA sequencing, Next-Generation Sequencing (NGS), DNA microarray and Southern blot.
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Functional Studies - Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (AB117133)
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Supplier Data

Functional Studies - Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (AB117133)

For enrichment of methylated DNA using ab117133, DNA (0.5 ug) isolated from MCF-7 cells was added into the microwell. Methylated DNA was captured by 5-mC antibody prebound to the microwells.

Functional Studies - Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (AB117133)
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PubMed

Functional Studies - Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (AB117133)

Functional Studies.

MeDIP was performed using 1μg of DNA with ab117133. Real-time PCR was carried out with MeDIP DNAs and the frequency of DNA methylation of immunoprecipitated DNA vs input DNA was calculated.

Han B et al., PLoS One, 11(8). Fig 5b. doi: 10.1371/journal.pone.0160358 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Functional Studies - Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (AB117133)
  • FuncS

Supplier Data

Functional Studies - Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (AB117133)

Captured methylated DNA was used for analyzing methylation level of GAPDH and MLH1 promoter with the use of primers and probes specific to GAPDH and MLH1 promoters, respectively.

Key facts

Assay time

3h

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Sample Prep & Detection Kits

AB117135

Methylated DNA Immunoprecipitation (MeDIP) ChIP Kit

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Product details

Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (ab117133) enables the user to enrich methylated DNA by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.

DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.

NOTE: the size of this kit is based on number of TESTS not SAMPLES. A test simply refers to a single assay well. If you are not sure which size is most suitable for you, contact our Scientific Support Team (technical@abcam.com).

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Hypertension (Dallas, Tex. : 1979) 82:293-305 PubMed39633580

2024

Epigenetic Upregulation of Carotid Body Angiotensin Signaling Increases Blood Pressure.

Applications

Unspecified application

Species

Unspecified reactive species

Fengli Zhu,Zhuqing Wang,Kayla Davis,Hayden McSwiggin,Jekaterina Zyuzin,Jie Liu,Wei Yan,Virender K Rehan,Nicholas Jendzjowsky

Frontiers in molecular biosciences 9:857491 PubMed35517863

2022

N-3 Polyunsaturated Fatty Acid Dehydrogenase Fat-1 Regulates Mitochondrial Energy Metabolism by Altering DNA Methylation in Isolated Cells of Transgenic Cattle.

Applications

Unspecified application

Species

Unspecified reactive species

Xueqiao Wang,Lin Zhu,Zhuying Wei,Mingjuan Gu,Miaomiao Yang,Xinyu Zhou,Chunling Bai,Guanghua Su,Xuefei Liu,Lei Yang,Guangpeng Li

Journal of psychopharmacology (Oxford, England) 34:680-692 PubMed32338111

2020

Cocaine-induced changes in behaviour and DNA methylation in rats are influenced by inter-individual differences in spontaneous exploratory activity.

Applications

Unspecified application

Species

Unspecified reactive species

Kadi Vaher,Kaili Anier,Monika Jürgenson,Jaanus Harro,Anti Kalda

International journal of biological sciences 16:1324-1334 PubMed32210722

2020

Mutant Promotes Myogenic Differentiation by Increasing Demethylase Expression via the SMAD2/SMAD3 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li Gao,Miaomiao Yang,Zhuying Wei,Mingjuan Gu,Lei Yang,Chunling Bai,Yunxi Wu,Guangpeng Li

Pharmaceutical biology 57:453-459 PubMed31311385

2019

Epigenetic mechanisms underlying the effects of triptolide and tripchlorolide on the expression of neuroligin-1 in the hippocampus of APP/PS1 transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomei Lu,Baolin Yang,Hao Yu,Xiaoling Hu,Jing Nie,Bin Wan,Ming Zhang,Cheng Lü

International journal of molecular sciences 20: PubMed31248224

2019

Myeloid-Specific Deletion of the AMPKα2 Subunit Alters Monocyte Protein Expression and Atherogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Beate Fisslthaler,Nina Zippel,Randa Abdel Malik,Fredy Delgado Lagos,Sven Zukunft,Janina Thoele,Daniel Siuda,Oliver Soehnlein,Ilka Wittig,Juliana Heidler,Andreas Weigert,Ingrid Fleming

American journal of physiology. Regulatory, integr 317:R397-R406 PubMed31188650

2019

Skeletal muscle LINE-1 retrotransposon activity is upregulated in older versus younger rats.

Applications

Unspecified application

Species

Unspecified reactive species

Petey W Mumford,Matthew A Romero,Shelby C Osburn,Paul A Roberson,Christopher G Vann,Christopher B Mobley,Michael D Brown,Andreas N Kavazis,Kaelin C Young,Michael D Roberts

Epigenetics & chromatin 12:32 PubMed31164154

2019

Phosphorylation of TET2 by AMPK is indispensable in myogenic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Zhang,Xiaowen Guan,Un Lam Choi,Qiang Dong,Melody M T Lam,Jianming Zeng,Jun Xiong,Xianju Wang,Terence C W Poon,Hongjie Zhang,Xuanjun Zhang,Hailin Wang,Ruiyu Xie,Bing Zhu,Gang Li

Mobile DNA 10:17 PubMed31073336

2019

Insertion of a chimeric retrotransposon sequence in mouse locus causes metastable kinky tail phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuqing Wang,Hayden McSwiggin,Simon J Newkirk,Yue Wang,Daniel Oliver,Chong Tang,Sandy Lee,Shawn Wang,Shuiqiao Yuan,Huili Zheng,Ping Ye,Wenfeng An,Wei Yan

Journal of assisted reproduction and genetics 36:1029-1038 PubMed30820784

2019

Evaluation and statistical optimization of a method for methylated cell-free fetal DNA extraction from maternal plasma.

Applications

Unspecified application

Species

Unspecified reactive species

Mostafa Akbariqomi,Reza Heidari,Soraya Saleh Gargari,Mir Davood Omrani,Garshasb Rigi,Nafiseh Sadat Sanikhani,Hamid Kooshki,Fatemeh Mahmoudian,Mohammad Ali Mazlomi,Gholamreza Tavoosidana
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