Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (ab117133) enables the user to enrich methylated DNA by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP).
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- Frontiers in molecular biosciences 9:8574912022N-3 Polyunsaturated Fatty Acid Dehydrogenase Fat-1 Regulates Mitochondrial Energy Metabolism by Altering DNA Methylation in Isolated Cells of Transgenic Cattle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXueqiao Wang,Lin Zhu,Zhuying Wei,Mingjuan Gu,Miaomiao Yang,Xinyu Zhou,Chunling Bai,Guanghua Su,Xuefei Liu,Lei Yang,Guangpeng LiPubMed 35517863
- Journal of psychopharmacology (Oxford, England) 34:680-6922020Cocaine-induced changes in behaviour and DNA methylation in rats are influenced by inter-individual differences in spontaneous exploratory activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKadi Vaher et. al.PubMed 32338111
- International journal of biological sciences 16:1324-13342020Mutant Promotes Myogenic Differentiation by Increasing Demethylase Expression via the SMAD2/SMAD3 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Gao et. al.PubMed 32210722
- Pharmaceutical biology 57:453-4592019Epigenetic mechanisms underlying the effects of triptolide and tripchlorolide on the expression of neuroligin-1 in the hippocampus of APP/PS1 transgenic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaomei Lu,Baolin Yang,Hao Yu,Xiaoling Hu,Jing Nie,Bin Wan,Ming Zhang,Cheng LüPubMed 31311385
- International journal of molecular sciences 20:2019Myeloid-Specific Deletion of the AMPKα2 Subunit Alters Monocyte Protein Expression and Atherogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBeate Fisslthaler et. al.PubMed 31248224
- American journal of physiology. Regulatory, integr 317:R397-R4062019Skeletal muscle LINE-1 retrotransposon activity is upregulated in older versus younger rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPetey W Mumford et. al.PubMed 31188650
- Epigenetics & chromatin 12:322019Phosphorylation of TET2 by AMPK is indispensable in myogenic differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Zhang et. al.PubMed 31164154
- Mobile DNA 10:172019Insertion of a chimeric retrotransposon sequence in mouse locus causes metastable kinky tail phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhuqing Wang et. al.PubMed 31073336
- Journal of assisted reproduction and genetics 36:1029-10382019Evaluation and statistical optimization of a method for methylated cell-free fetal DNA extraction from maternal plasma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMostafa Akbariqomi et. al.PubMed 30820784
- PloS one 11:e01603582016Variation of DNA Methylome of Zebrafish Cells under Cold Pressure.
Key facts
- Assay time
- 3h
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Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (ab117133) enables the user to enrich methylated DNA by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP).
Key facts
- Assay time
- 3h
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (ab117133) enables the user to enrich methylated DNA by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.
DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.
NOTE: the size of this kit is based on number of TESTS not SAMPLES. A test simply refers to a single assay well. If you are not sure which size is most suitable for you, contact our Scientific Support Team (technical@abcam.com).
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Han B et al., PLoS One, 11(8). Fig 5b. doi: 10.1371/journal.pone.0160358 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (ab117133)
Functional Studies.
MeDIP was performed using 1μg of DNA with ab117133. Real-time PCR was carried out with MeDIP DNAs and the frequency of DNA methylation of immunoprecipitated DNA vs input DNA was calculated.
Functional Studies - Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (ab117133)
Captured methylated DNA was used for analyzing methylation level of GAPDH and MLH1 promoter with the use of primers and probes specific to GAPDH and MLH1 promoters, respectively.
Functional Studies - Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (ab117133)
For enrichment of methylated DNA using ab117133, DNA (0.5 ug) isolated from MCF-7 cells was added into the microwell. Methylated DNA was captured by 5-mC antibody prebound to the microwells.
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