JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB117136

Methylated DNA Immunoprecipitation (MeDIP) Kit - Tissue

Be the first to review this product! Submit a review

|

(0 Publication)

Methylated DNA Immunoprecipitation (MeDIP) Kit - Tissue (ab117136) contains reagents required for carrying out methylated DNA immunoprecipitation directly from mammalian tissue.

- Includes a positive control antibody (5-methylcytosine) and negative control (Non-immune IgG)
- Highly efficient enrichment of methylated DNA: >95%
- Compatible with various downstream applications including qPCR, MS-PCR, DNA sequencing, Southern blot and DNA microarray.

Key facts

Assay time

3h 30m

Sample types

Tissue

Reacts with

Mouse, Human

style
left-column

Complementary Products

Sample Prep & Detection Kits

AB117133

Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA

Functional Studies - Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (AB117133)

  • 0
  • 0
Assay Kits

AB117128

Methylated DNA Quantification Kit (Colorimetric)

Functional Studies - Methylated DNA Quantification Kit (Colorimetric) (AB117128)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB113467

DNMT Activity Assay Kit (Colorimetric)

Functional Studies - DNMT Activity Assay Kit (Colorimetric) (AB113467)

  • 4
  • 2
style
left-column

Product details

DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.

Methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117136) enables the user to enrich methylated DNA directly from various mammalian tissues by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.

style
left-column

What's included?

{ "values": { "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab117136-48Test", "assetComponentDetails": [ { "size":"1 x 50 µL", "name":"Proteinase K (10 mg/mL)", "number":"AB117136-CMP08" }, { "size":"1 x 10 µL", "name":"Normal Mouse IgG (1 mg/mL)", "number":"AB117136-CMP07" }, { "size":"1 x 10 mL", "name":"Homogenizing Buffer", "number":"AB117136-CMP16" }, { "size":"1 x 6 mL", "name":"ChIP dilution buffer", "number":"AB117136-CMP06" }, { "size":"6 x 1 Unit", "name":"8-Well Strip Caps", "number":"AB117136-CMP03" }, { "size":"50 x 1 Unit", "name":"F-Spin Column", "number":"AB117136-CMP15" }, { "size":"50 x 1 Unit", "name":"F-Collection Tube", "number":"AB117136-CMP14" }, { "size":"2 x 2 mL", "name":"DNA release buffer", "number":"AB117136-CMP09" }, { "size":"1 x 30 mL", "name":"Antibody buffer", "number":"AB117136-CMP04" }, { "size":"6 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB117136-CMP01" }, { "size":"2 x 28 mL", "name":"Wash buffer", "number":"AB117136-CMP13" }, { "size":"1 x 8 mL", "name":"Binding buffer", "number":"AB117136-CMP05" }, { "size":"1 x 1.2 mL", "name":"Elution buffer", "number":"AB117136-CMP10" }, { "size":"1 x 6 mL", "name":"Lysis buffer", "number":"AB117136-CMP11" }, { "size":"2 x 2 mL", "name":"Reverse buffer", "number":"AB117136-CMP12" }, { "size":"1 x 50 µL", "name":"Anti-5-methylcytosine (1mg/mL)", "number":"AB117136-CMP02" } ] }, "24Test": { "sellingSize": "24 Test", "publicAssetCode":"ab117136-24Test", "assetComponentDetails": [ { "size":"1 x 25 µL", "name":"Proteinase K (10 mg/mL)", "number":"AB117136-CMP08", "productcode":"" }, { "size":"1 x 28 mL", "name":"Wash buffer", "number":"AB117136-CMP13", "productcode":"" }, { "size":"1 x 2 mL", "name":"Reverse buffer", "number":"AB117136-CMP12", "productcode":"" }, { "size":"1 x 4 mL", "name":"Lysis buffer", "number":"AB117136-CMP11", "productcode":"" }, { "size":"1 x 2 mL", "name":"DNA release buffer", "number":"AB117136-CMP09", "productcode":"" }, { "size":"1 x 5 mL", "name":"Homogenizing Buffer", "number":"AB117136-CMP16", "productcode":"" }, { "size":"1 x 25 µL", "name":"Anti-5-methylcytosine (1mg/mL)", "number":"AB117136-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"Antibody buffer", "number":"AB117136-CMP04", "productcode":"" }, { "size":"30 x 1 Unit", "name":"F-Spin Column", "number":"AB117136-CMP15", "productcode":"" }, { "size":"3 x 1 Unit", "name":"8-Well Strip Caps", "number":"AB117136-CMP03", "productcode":"" }, { "size":"1 x 0.6 mL", "name":"Elution buffer", "number":"AB117136-CMP10", "productcode":"" }, { "size":"1 x 10 µL", "name":"Normal Mouse IgG (1 mg/mL)", "number":"AB117136-CMP07", "productcode":"" }, { "size":"1 x 5 mL", "name":"Binding buffer", "number":"AB117136-CMP05", "productcode":"" }, { "size":"30 x 1 Unit", "name":"F-Collection Tube", "number":"AB117136-CMP14", "productcode":"" }, { "size":"1 x 4 mL", "name":"ChIP dilution buffer", "number":"AB117136-CMP06", "productcode":"" }, { "size":"3 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB117136-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com