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This Nuclear/Cytosol Extraction Kit (ab289882, K266) provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

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Sample type

Tissue, Suspension cells, Adherent cells

Reactive species

Mammals

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What's included?

100 Tests
Components
DTT II
1 x 100 µL
Extraction Buffer V
1 x 10 mL
Extraction Buffer VI
1 x 20 mL
Lysis Buffer X
1 x 1.2 mL
Protease Inhibitor Cocktail I
1 x 1 Vial

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This Nuclear/Cytosol Extraction Kit (ab289882, K266) provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Sample type

Tissue, Suspension cells, Adherent cells

Reactive species

Mammals

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

This Nuclear/Cytosol Extraction Kit (ab289882, K266) provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells. The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic fractions quickly with little or no cross-contaminations. The extracted nuclear and cytoplasmic protein fractions are functional and compatible with downstream assays such as transcriptional activity, RNA splicing, gel shift assay, reporter assays, enzyme activity assays, and Western blotting.


  • Simple procedure; takes less than 2-3 hours

  • The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic extracts from cells relatively quick with little or no cross-contaminations

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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