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Nuclear extraction kit ab219177 offers a simple 45 minute method for extracting nuclear proteins from mammalian cells or tissues.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

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Assay time

45m

Sample type

Adherent cells, Suspension cells, Tissue

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Images

What's included?

100 Test
Components
200X DTT
1 x 1 mL
Cytoplasm Extraction Buffer
1 x 55 mL
Nuclear Extraction Buffer
1 x 55 mL
Nuclear Lysis Buffer
1 x 55 mL
Protease Inhibitor Cocktail
1 x 1 mL

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Nuclear extraction kit ab219177 offers a simple 45 minute method for extracting nuclear proteins from mammalian cells or tissues.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Assay time

45m

Sample type

Adherent cells, Suspension cells, Tissue

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Nuclear extraction kit ab219177 offers a simple 45 minute method for extracting nuclear proteins from mammalian cells or tissues.

For optimal extraction of nuclear proteins, the kit produces three distinct extracts (fractions): soluble nuclear proteins (nuclear extract 1), nuclear proteins that remain insoluble after production of nuclear extract 1 (nuclear extract 2), and cytoplasmic proteins.

The kit contains reagents for 100 extractions, where each extraction starts with 5 x 106 cells or 50 mg of tissue. Typical yields from each extraction are 0.3 – 0.4 mg of soluble nuclear proteins, 0.1 – 0.2 mg of insoluble nuclear proteins and 0.6 – 0.7 mg of cytoplasmic proteins. Nuclear proteins extracted with this kit can be used in a variety of applications such as western blotting and nuclear enzyme assays.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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