ab154470 is suitable to immunocapture p53 from whole cell lysates.
View Alternative Names
P53, TP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
- IP
Supplier Data
Immunoprecipitation - p53 Human Immunocapture Kit (AB154470)
p53 immunocapture kit (ab154470) is specific to the p53 protein. Control proteins HSP60 and actin are present in the Hek293 and MCF7 lysate inputs, but are not found in p53 immunoprecipiations.
Hek293 cells were extracted with lauryl maltoside (lanes 2, 9), RIPA buffer (lanes 3, 10) or SDS (lanes 4, 11). MCF7 vehicle-treated cells were extracted with lauryl maltoside (lanes 5, 12) or RIPA buffer (lanes 7, 14). MCF7 cells treated with 1 μM camptothecin were extracted with lauryl maltoside (lanes 6, 13) or RIPA buffer (8, 15). Extracts of whole cells (20 μg, lanes 2-8), and one-fifth of immunoprecipitation samples using the p53 immunocapture beads (1 mg extract per 10 μL beads, lanes 9-15), were analyzed by Western blot using ab46798 anti-Hsp60 and ab46805 anti-muscle actin.
false
- IP
Supplier Data
Immunoprecipitation - p53 Human Immunocapture Kit (AB154470)
Western blot with ab154470 and MCF7 camptothecin-treated cells All lanes : ab32389 anti-p53 1/1000 Lane 1 : MW marker Lane 2 : MCF7 cells extracted with lauryl maltoside (LM). Lane 3 : MCF7 cells treated with 1 µM camptothecin for 6 hrs, extracted with LM. Lane 4 : MCF7 cells extracted with RIPA buffer. Lane 5 : MCF7 cells treated with 1 µM camptothecin for 6 hours, extracted with RIPA buffer. Lane 6 : IP with 10 µL p53 IP beads and 1 mg MCF7 cells, LM extract. Lane 7 : IP with 10 µL p53 IP beads and 1 mg MCF7 cells treated with 1 µM camptothecin for 6 hrs, LM extract. Lane 8 : IP with 10 µL p53 IP beads and 1 mg MCF7 cells, RIPA extract. Lane 9 : IP with 10 µL p53 IP beads and 1 mg MCF7 cells treated with 1 µM camptothecin for 6 hrs, RIPA extract. Lane 10 : IP with 10 µL ab135397 beads and 1 mg MCF7 cells, RIPA extract. Lane 11 : IP with 10 µL ab135397 beads and 1 mg MCF7 cells treated with 1 µM camptothecin for 6 hours, RIPA extract.
false
- IP
Supplier Data
Immunoprecipitation - p53 Human Immunocapture Kit (AB154470)
false
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Reactivity data
Product details
ab154470 is suitable to immunocapture p53 from whole cell lysates. Traditional immunoprecipitation methods usually result in co-elution of the antibody heavy and light chains that may co-migrate with relevant bands, masking important results. The abcam p53 immunocapture kit resolves this issue by immobilizing the p53 capture antibody onto protein G-agarose beads. The kit includes optimized buffers and reagents for sample preparation and p53 binding and recovery, which shorten the protocol and minimize handling and mixing.
p53 acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction by p53 seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. p53 is implicated in Notch signaling cross-over. p53 prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression.
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Supplementary information
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Biological function summary
P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.
Pathways
P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.
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Publications (2)
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Cell reports 42:112024 PubMed36848235
2023
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Unspecified reactive species
The Journal of biological chemistry 288:36662-75 PubMed24178296
2013
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Human
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