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ab154470 is suitable to immunocapture p53 from whole cell lysates.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

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Sample type

Tissue Extracts, Tissue Homogenate, Cell Lysate

Reactive species

Human

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Reactivity data

Application
IP
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

What's included?

200 µg
Components
10X Extraction Buffer
1 x 1 mL
10X PBS
1 x 10 mL
10X Wash Buffer
1 x 10 mL
Immunocapture p53 antibody coupled to agarose beads
1 x 200 µg
SDS Elution Buffer
1 x 1 mL

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ab154470 is suitable to immunocapture p53 from whole cell lysates.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Sample type

Tissue Extracts, Tissue Homogenate, Cell Lysate

Reactive species

Human

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

ab154470 is suitable to immunocapture p53 from whole cell lysates. Traditional immunoprecipitation methods usually result in co-elution of the antibody heavy and light chains that may co-migrate with relevant bands, masking important results. The abcam p53 immunocapture kit resolves this issue by immobilizing the p53 capture antibody onto protein G-agarose beads. The kit includes optimized buffers and reagents for sample preparation and p53 binding and recovery, which shorten the protocol and minimize handling and mixing.

p53 acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction by p53 seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. p53 is implicated in Notch signaling cross-over. p53 prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression.

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Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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