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Mono AEC/Plus is a single, highly stable AEC chromogen/substrate working solution.

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Mono AEC/Plus is a single, highly stable AEC chromogen/substrate working solution.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Please refer to protocols

Notes

Mono AEC/Plus is a single, highly stable AEC chromogen/substrate working solution. When used in conjugation with immunoperoxidase detection systems, AEC produces a red colored and product at positive sites, yeilding strong contrast when combined with a blue hematoxylin counterstain. Specimens stained using Mono AEC/Plus cannot be dehydrated in ethanol and musy be mounted in an aqueous-based mounting medium.

ab103742 is a single, stable ready for use solution. The solution is light sensitive and must be protected from exposure to light and stored in an opaque bottle or dark environment.

Protocol

  1. Once section have been incubated with peroxidase, wash with wash buffer.
  2. Wipe slides removing excess buffer. Add enough drops of Mono AEC/Plus to cover tissue sections.
  3. Incubate for 5-15 minutes at room temperature. For optimal results, observe reaction under the microscope for signal developmant. Once the desired signal to noise ratio is acheived, stop the reaction by washing the slides in DI H2O.

AEC Single/Plus is a single, highly stable AEC chromogen/substrate working solution. When used in conjugation with immunoperoxidase detection systems, AEC produces a red colored and product at positive sites, yeilding strong contrast when combined with a blue hematoxylin counterstain. Specimens stained using AEC Single/Plus cannot be dehydrated in ethanol and must be mounted in an aqueous-based mounting medium.

AEC Single/Plus (ab103742) is a single, stable ready for use solution. The solution is light sensitive and must be protected from exposure to light and stored in an opaque bottle or dark environment.

Protocol

  • Once section have been incubated with peroxidase, wash with wash buffer.
  • Wipe slides removing excess buffer. Add enough drops of AEC Single/Plus to cover tissue sections.
  • Incubate for 5-15 minutes at room temperature. For optimal results, observe reaction under the microscope for signal developmant. Once the desired signal to noise ratio is acheived, stop the reaction by washing the slides in DI H2O.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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