AEC substrate kit for use in IHC using HRP / peroxidase detection.
Images
Publications
Filter by
- Balkan medical journal 41:30-372024Quercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYeter Topçu-Tarladaçalışır,Melike Sapmaz-Metin,Zeynep Mercan,Deniz ErçetinPubMed 38173174
- Nature communications 14:56032023A Lassa virus mRNA vaccine confers protection but does not require neutralizing antibody in a guinea pig model of infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdam J Ronk,Nicole M Lloyd,Min Zhang,Caroline Atyeo,Hailee R Perrett,Chad E Mire,Kathryn M Hastie,Rogier W Sanders,Philip J M Brouwer,Erica Olmann Saphire,Andrew B Ward,Thomas G Ksiazek,Juan Carlos Alvarez Moreno,Harshwardhan M Thaker,Galit Alter,Sunny Himansu,Andrea Carfi,Alexander BukreyevPubMed 37699929
- Frontiers in veterinary science 10:11558042023Whole transcriptome analysis of canine pheochromocytoma and paraganglioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarit F van den Berg,Hans S Kooistra,Guy C M Grinwis,Stefano Nicoli,Stefania Golinelli,Lisa Stammeleer,Monique E van Wolferen,Elpetra P M Timmermans-Sprang,Maurice M J M Zandvliet,Frank G van Steenbeek,Sara GalacPubMed 37691636
- The Journal of biological chemistry 299:1048682023Aβ43 levels determine the onset of pathological amyloid deposition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarc D Tambini,Tao Yin,Metin Yesiltepe,Lionel Breuillaud,Simone P Zehntner,Cristina d'Abramo,Luca Giliberto,Luciano D'AdamioPubMed 37257821
- eLife 12:2023Skeletal dysplasia-causing TRPV4 mutations suppress the hypertrophic differentiation of human iPSC-derived chondrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmanda R Dicks,Grigory I Maksaev,Zainab Harissa,Alireza Savadipour,Ruhang Tang,Nancy Steward,Wolfgang Liedtke,Colin G Nichols,Chia-Lung Wu,Farshid GuilakPubMed 36810131
- Developmental cell 58:34-50.e92023A Cdh3-β-catenin-laminin signaling axis in a subset of breast tumor leader cells control leader cell polarization and directional collective migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPriscilla Y Hwang,Jairaj Mathur,Yanyang Cao,Jose Almeida,Jiayu Ye,Vasilios Morikis,Daphne Cornish,Maria Clarke,Sheila A Stewart,Amit Pathak,Gregory D LongmorePubMed 36626870
- Frontiers in pharmacology 13:10296362022Viral delivery of a peptide-based immunomodulator enhances T cell priming during vaccination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTimothy W Phares,Jing Huang,Vinayaka Kotraiah,Mary J Hauser,Arban Domi,Sreenivasa Oruganti,Cecille D Browne,Peter Buontempo,Marc Mansour,James Pannucci,Moriya Tsuji,Gabriel M GutierrezPubMed 36582528
- Nature cardiovascular research 1:1174-11862022Loss of soluble guanylyl cyclase in platelets contributes to atherosclerotic plaque formation and vascular inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarina Mauersberger,Hendrik B Sager,Jana Wobst,Tan An Dang,Laura Lambrecht,Simon Koplev,Marlène Stroth,Noomen Bettaga,Jens Schlossmann,Frank Wunder,Andreas Friebe,Johan L M Björkegren,Lisa Dietz,Sanne L Maas,Emiel P C van der Vorst,Peter Sandner,Oliver Soehnlein,Heribert Schunkert,Thorsten KesslerPubMed 37484062
- Frontiers in aging neuroscience 14:10405762022Late-long-term potentiation magnitude, but not Aβ levels and amyloid pathology, is associated with behavioral performance in a rat knock-in model of Alzheimer disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMetin Yesiltepe,Tao Yin,Marc D Tambini,Lionel Breuillaud,Simone P Zehntner,Luciano D'AdamioPubMed 36438008
- International journal of molecular sciences 23:2022Evaluating Thera-101 as a Low-Volume Resuscitation Fluid in a Model of Polytrauma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica Stukel Shah,Joseph Macaitis,Bridney Lundquist,Brian Johnstone,Michael Coleman,Michelle A Jefferson,Jacob Glaser,Annette R Rodriguez,Sylvain Cardin,Heuy-Ching Wang,Alexander BurdettePubMed 36293520
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Recommended products
AEC substrate kit for use in IHC using HRP / peroxidase detection.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Please refer to protocols
Notes
AEC substrate kit for use in IHC using HRP / peroxidase detection. Ready to use formulation.
Previously called AEC Substrate System (Ready to Use).
IHC protocol suitable for use with AEC Substrate Kit ab64252:
1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
2. Use appropriate antigen retrieval buffer or enzyme (primary antibody dependent) to treat sections. Wash 3 times in buffer.
3. Add enough hydrogen peroxide blocking solution to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use avidin biotin blocking.
4. Apply protein block (or normal serum from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.
5. Apply primary antibody in antibody diluent and incubate.
6. Wash 4 times in buffer. Incubate slide with biotinylated secondary antibody (or HRP polymer secondary antibody and skip step 7). Wash 4 times in buffer.
7. Apply streptavidin-HRP and incubate for 10 minutes at room temperature.
8. Rinse 4 times in buffer. Place slide inDAB substrate or AEC Substrate ab64252 and incubate until desired color is achieved (1-10 mins, recommended 10 mins for AEC). Rinse 4 times in buffer.
9. Add enough drops of hematoxylin to cover the section. Incubate for 1 minute.
10. Rinse 7-8 times in tap water. Add mounting medium to cover the section.
Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Image from Kryza et al. PLoS One; 11(2) :e0149387. doi: 10.1371/journal.pone.0149387. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AEC Substrate Kit (ab64252)
Immunostaining using anti-hMMP-9 murine monoclonal antibody.Slides of superficial spread melanoma tumors were incubated with the hMMP-9 antibody (Anti-MMP9 antibody [56-2A4] ab58803, 1:300). Alkanie phosphatas esecondary antibody was stained with AEC (3-Amino-9-EthylCarbazole, ab 64252, Abcam, Cambridge) for 15 min and slides were counterstained with hematoxylin for examination.
Image from Trus et al., Viruses; 12(5):579. doi: 10.3390/v12050579. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Frozen sections) - AEC Substrate Kit (ab64252)
Immunostaining for TGM2 and SOX2 staining in GSC 158 and GSC 157 cells.(a) Positive TGM2 and negative SOX2 staining in GSC 158 cells. (b) Negative TGM2 and positive SOX2 staining in GSC 157 cells. Cells were incubated with primary monoclonal Abs for 1 h at +37 °C (for SOX2, Rabbit Anti-Rat IgG H&L (HRP) ab6734, 1/200) following the Lab Vision Ready-To-Use AEC Substrate System (ab64252) according to the manufacturer's instructions.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AEC Substrate Kit (ab64252)
Human colon carcinoma stained with anti-Keratin 20 antibody using AEC Substrate System (Ready to Use) (ab64252).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com