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AB7413

Alkaline Phosphatase chromogen (BCIP/TNBT)

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Alkaline Phosphatase chromogen (BCIP/TNBT) (ab7413) is a quick, easy-to-use liquid substrate for alkaline phosphatase (AP), and is especially useful for the detection of AP-conjugated antibodies in Western blot, IHC and ISH.

- Ready-to-use solution.

Key facts

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product is a liquid substrate for alkaline phosphatase (AP), and is especially useful for the detection of AP-conjugated antibodies (i.e. secondary antibodies). Apply to tissue section for 10 minutes or until a suitable level of colouration has been achieved.

BCIP: 5-bromo-4-chloro-3-indolyl phosphate 0.452 mM TNBT: tetranitroblue tetrazolium 0.432 mM This product used to be called "Alkaline phosphatase detection kit (BCIP/TNBT)".

BCIP/TNBT is similar to BCIP/NBT but is more sensitive and produces a more intense purple color. A useful secondary marker in multiple staining procedures.

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Additional targets

Alkaline Phosphatase Chromogen
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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