This product is a liquid substrate for alkaline phosphatase (AP), and is especially useful for the detection of AP-conjugated antibodies.
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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This product is a liquid substrate for alkaline phosphatase (AP), and is especially useful for the detection of AP-conjugated antibodies.
This product is a liquid substrate for alkaline phosphatase (AP), and is especially useful for the detection of AP-conjugated antibodies (i.e. secondary antibodies). Apply to tissue section for 10 minutes or until a suitable level of colouration has been achieved.
BCIP: 5-bromo-4-chloro-3-indolyl phosphate 0.452 mM TNBT: tetranitroblue tetrazolium 0.432 mM This product used to be called "Alkaline phosphatase detection kit (BCIP/TNBT)".
BCIP/TNBT is similar to BCIP/NBT but is more sensitive and produces a more intense purple color. A useful secondary marker in multiple staining procedures.
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