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DAB substrate kit ab64238 is used for IHC staining using HRP / peroxidase enzyme for detection.

Reactivity data

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Images

What's included?

60 mL
Components
50x DAB Chromogen
1 x 2 mL
DAB substrate
1 x 60 mL

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DAB substrate kit ab64238 is used for IHC staining using HRP / peroxidase enzyme for detection.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

DAB substrate kit ab64238 is used for IHC staining using HRP / peroxidase enzyme for detection. Reagent is stable for up to 6 hours after mixing the two components.DAB (3,3'Diaminobenzidine) is the most commonly used chromogen for immunohistochemical staining. In the presence of HRP / peroxidase, DAB produces a brown precipitate that is insoluble in alcohol.This product is a two component form consisting of a liquid, refrigerator stable DAB Chromogen and DAB Substrate. **IHC protocol suitable for use with DAB substrate kit:**For frozen sections, skip steps 1 and 2. 1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.2. Use appropriate (primary antibody dependent) to treat sections. Wash 3 times in buffer.3. Add enough to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use .4. Apply (or from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.5. Apply primary antibody in and incubate.6. Wash 4 times in buffer. Incubate slide with (or and skip step 7). Wash 4 times in buffer.7. Apply and incubate for 10 minutes at room temperature.8. Rinse 4 times in buffer. **Place slide in DAB substrate ab64238** or ** and incubate until desired color is achieved (1-10 mins)**. Rinse 4 times in buffer.9. Add enough drops of to cover the section. Incubate for 1 minute.10. Rinse 7-8 times in tap water. Add to cover the section.

Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the .

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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