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DAB substrate kit is used for immunohistochemistry (IHC) staining using HRP/peroxidase enzyme for detection.

- Cited in over 375 publications
- Stable for up to 6 hours after mixing
- Produces crisp brown stain, alcohol-insoluble
- Convenient two-component liquid format
- Refrigerator-stable for extended usability


Images

Immunohistochemistry - DAB Substrate Kit (AB64238), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAB Substrate Kit (AB64238), expandable thumbnail

Publications

Reactivity data

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

What's included?

60 mL
Components
50x DAB Chromogen
1 x 2 mL
DAB substrate
1 x 60 mL

Recommended products

DAB substrate kit is used for immunohistochemistry (IHC) staining using HRP/peroxidase enzyme for detection.

- Cited in over 375 publications
- Stable for up to 6 hours after mixing
- Produces crisp brown stain, alcohol-insoluble
- Convenient two-component liquid format
- Refrigerator-stable for extended usability

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

DAB substrate kit is used for immunohistochemistry (IHC) staining using HRP/peroxidase enzyme for detection.

Reagent is stable for up to 6 hours after mixing the two components.

DAB (3,3'Diaminobenzidine) is the most commonly used chromogen for immunohistochemical staining. In the presence of HRP / peroxidase, DAB produces a brown precipitate that is insoluble in alcohol.This product is a two component form consisting of a liquid, refrigerator stable DAB Chromogen and DAB Substrate.


Staining Protocol (see protocol booklet for further details):
Equilibrate all materials and prepared reagents to room temperature prior to use
1. Add 30 μl (1 drop) DAB Chromogen to 1.5 ml (50 drops) of DAB Substrate, mix by swirling and apply to tissue.
2. Incubate for 1-10 minutes. Rinse 4 times in buffer.
3. Apply counterstain according to manufacturer’s instructions.
4. Dehydrate if required and coverslip.

Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the .

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2 product images

  • Immunohistochemistry - DAB Substrate Kit (ab64238), expandable thumbnail
    Lean QY et al. PLoS One (2015) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunohistochemistry - DAB Substrate Kit (ab64238)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Immunohistochemical analysis of healthy and colitis mouse colon sections (untreated and treated with enoxaparin) labeling claudin-4 with Anti-Claudin 4 antibody ab15104 at 1/200. Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab7090, anti-rabbit immunoglobulin G conjugated to horseradish peroxidase (HRP) at 1/300 was used as the secondary antibody. ab64238 at 1/50 was used to develop the histological signal.

    Antigen retrieval was performed by incubating the sections for 10 minutes at 97°C in 1 mM EDTA buffer, pH 8.0 or 10 mM citrate buffer, pH 6.0.

    Control, C; untreated colitis, DSS; oral enoxaparin, OE; intraperitoneal injection of enoxaparin, IPE.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAB Substrate Kit (ab64238), expandable thumbnail
    Jujo T et al. PLoS One (2014) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAB Substrate Kit (ab64238)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Immunohistochemical analysis of mouse lung. Samples were fixed in 10% buffered formalin, paraffinized and sliced at 1.5 μm thick. Antigen retrieval was performed using 10x Citrate Buffer pH 6.0 ab64214 for the deparaffinized slices. Sections were blocked with 2% normal goat serum, PBS(-) and 0.1% Tween20. They were then incubated with the primary antibodies for 1 hour at 4°C and with secondary antibodies for 30 minutes at room temperature. The avidin-biotin-peroxidase complex method with peroxidase streptavidin and the DAB substrate kit ab64238 was performed.

    (A) A resected lung from a mouse sacrificed 28 days after SCL injection.

    (B) Haemotoxylin and Eosin staining showing the tumor composed of a central area with necrosis and a peripheral zone filled with SCLs.

    (C) Immunohistochemical staining for MMP-14 showing positive expression of MMP-14 in the peripheral zone of the tumor and negative in central zone.

    (D) Immunohistochemical staining weak staining of MMP-2 in the peripheral zone.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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