DAB substrate kit ab64238 is used for IHC staining using HRP / peroxidase enzyme for detection.
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Publications
- NPJ systems biology and applications 10:52024The antidepressive mechanism of Longya Lilium combined with Fluoxetine in mice with depression-like behaviors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuina Ma,Hehua Huang,Chenyu Li,Shasha Li,Juefang Gan,Chunrong Lian,Yanwu LingPubMed 38218856
- Molecular nutrition & food research 68:e23001232024Puerarin Attenuates Iron Overload-Induced Ferroptosis in Retina through a Nrf2-Mediated Mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiongtao Song,Wenyuan Jian,Yuanyuan Zhang,Qiang Li,Ying Zhao,Rong Liu,Yan Zeng,Fuwen Zhang,Junguo DuanPubMed 38196088
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
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DAB substrate kit ab64238 is used for IHC staining using HRP / peroxidase enzyme for detection.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
DAB substrate kit ab64238 is used for IHC staining using HRP / peroxidase enzyme for detection. Reagent is stable for up to 6 hours after mixing the two components.DAB (3,3'Diaminobenzidine) is the most commonly used chromogen for immunohistochemical staining. In the presence of HRP / peroxidase, DAB produces a brown precipitate that is insoluble in alcohol.This product is a two component form consisting of a liquid, refrigerator stable DAB Chromogen and DAB Substrate. **IHC protocol suitable for use with DAB substrate kit:**For frozen sections, skip steps 1 and 2. 1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.2. Use appropriate (primary antibody dependent) to treat sections. Wash 3 times in buffer.3. Add enough to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use .4. Apply (or from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.5. Apply primary antibody in and incubate.6. Wash 4 times in buffer. Incubate slide with (or and skip step 7). Wash 4 times in buffer.7. Apply and incubate for 10 minutes at room temperature.8. Rinse 4 times in buffer. **Place slide in DAB substrate ab64238** or ** and incubate until desired color is achieved (1-10 mins)**. Rinse 4 times in buffer.9. Add enough drops of to cover the section. Incubate for 1 minute.10. Rinse 7-8 times in tap water. Add to cover the section.
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2 product images
Lean QY et al. PLoS One (2015) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemistry - DAB Substrate Kit (ab64238)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Immunohistochemical analysis of healthy and colitis mouse colon sections (untreated and treated with enoxaparin) labeling claudin-4 with Anti-Claudin 4 antibody ab15104 at 1/200. Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab7090, anti-rabbit immunoglobulin G conjugated to horseradish peroxidase (HRP) at 1/300 was used as the secondary antibody. ab64238 at 1/50 was used to develop the histological signal.
Antigen retrieval was performed by incubating the sections for 10 minutes at 97°C in 1 mM EDTA buffer, pH 8.0 or 10 mM citrate buffer, pH 6.0.
Control, C; untreated colitis, DSS; oral enoxaparin, OE; intraperitoneal injection of enoxaparin, IPE.
Jujo T et al. PLoS One (2014) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAB Substrate Kit (ab64238)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Immunohistochemical analysis of mouse lung. Samples were fixed in 10% buffered formalin, paraffinized and sliced at 1.5 μm thick. Antigen retrieval was performed using 10x Citrate Buffer pH 6.0 ab64214 for the deparaffinized slices. Sections were blocked with 2% normal goat serum, PBS(-) and 0.1% Tween20. They were then incubated with the primary antibodies for 1 hour at 4°C and with secondary antibodies for 30 minutes at room temperature. The avidin-biotin-peroxidase complex method with peroxidase streptavidin and the DAB substrate kit ab64238 was performed.
(A) A resected lung from a mouse sacrificed 28 days after SCL injection.
(B) Haemotoxylin and Eosin staining showing the tumor composed of a central area with necrosis and a peripheral zone filled with SCLs.
(C) Immunohistochemical staining for MMP-14 showing positive expression of MMP-14 in the peripheral zone of the tumor and negative in central zone.
(D) Immunohistochemical staining weak staining of MMP-2 in the peripheral zone.
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