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NBT / BCIP chromogen (ab7468): a sensitive substrate for alkaline phosphatase detection in immunoblotting.

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Reactivity data

Application
Dot
Reactivity
Reacts
Dilution info
-
Notes

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Application
WB
Reactivity
Reacts
Dilution info
-
Notes

BCIP/NBT system is based on hydrolysis of BCIP and reduction of NBT producing a deep purple reaction product. Reaction is several times more sensitive than existing products with little or no background staining. The reaction product is extremely stable and does not fade when exposed to light.

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NBT / BCIP chromogen (ab7468): a sensitive substrate for alkaline phosphatase detection in immunoblotting.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Please refer to protocols

Notes

NBT / BCIP chromogen (ab7468): a sensitive substrate for alkaline phosphatase detection in immunoblotting. Ready to use formulation.

BCIP: 5-bromo-4-chloro-3-indolyl phosphate NBT: p-nitroblue tetrazolium chloride.

This product used to be called "Alkaline phosphatase detection kit (BCIP/NBT)".

NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.

To use: Wash the membrane after incubation with the alkaline phosphatase conjugate secondary reagent. Apply sufficient substrate to cover the membrane. Color (purple-turquoise, varies with pH) will develop within 30 minutes. Wash, dry membrane and image.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

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