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AB7468

NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen

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(3 Publications)

NBT / BCIP Solution (ab7468 ) is an easy-to-use liquid substrate for alkaline phosphatase (AP) detection in immunoblotting assays e.g. Western blot.

- Ready-to-use solution.
4 Images
Dot Blot - NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (AB7468)
  • Dot

Supplier Data

Dot Blot - NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (AB7468)

Dot blot analysis of ab6742. Detected using Alkaline Phosphatase Substrate (ab7468).

All lanes:

Dot Blot - Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (<a href='/en-us/products/secondary-antibodies/rabbit-goat-igg-h-l-alkaline-phosphatase-ab6742'>ab6742</a>)

Lane 1:

Goat IgG at 200 ng

Lane 2:

Goat IgG at 66.67 ng

Lane 3:

Goat IgG at 22.2 ng

Lane 4:

Goat IgG at 7.4 ng

Lane 5:

Goat IgG at 2.47 ng

Secondary

All lanes:

Goat IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated at 1/1000 dilution

false

Dot Blot - NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (AB7468)
  • Dot

Supplier Data

Dot Blot - NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (AB7468)

Dot blot analysis of ab7140. Detected using Alkaline Phosphatase Substrate (ab7468) for 30 seconds at RT.

All lanes:

Dot Blot - Goat Anti-Guinea pig IgG H&L (Alkaline Phosphatase) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-guinea-pig-igg-h-l-alkaline-phosphatase-preadsorbed-ab7140'>ab7140</a>)

Lane 1:

Guinea Pig IgG at 200 ng

Lane 2:

Guinea Pig IgG at 66.67 ng

Lane 3:

Guinea Pig IgG at 22.2 ng

Lane 4:

Guinea Pig IgG at 7.4 ng

Lane 5:

Guinea Pig IgG at 2.47 ng

Secondary

All lanes:

Goat anti-Guinea Pig IgG Antibody (Min X 10) Alkaline Phosphatase Conjugated Antibody at 1/1000 dilution

false

Dot Blot - NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (AB7468)
  • Dot

Supplier Data

Dot Blot - NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (AB7468)

Dot blot analysis of ab7098. Detected using Alkaline Phosphatase Substrate (ab7468) for 30 seconds at RT.

All lanes:

Dot Blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-alkaline-phosphatase-preadsorbed-ab7098'>ab7098</a>) at 1/1000 dilution

Lane 1:

Rat IgG at 100 ng

Lane 2:

Rat IgG at 33.3 ng

Lane 3:

Rat IgG at 11.1 ng

Lane 4:

Rat IgG at 3.7 ng

Lane 5:

Rat IgG at 1.23 ng

false

Dot Blot - NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (AB7468)
  • Dot

Supplier Data

Dot Blot - NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (AB7468)

Dot blot analysis of ab6859. Detected using Alkaline Phosphatase Substrate (ab7468) for 30 seconds at RT.

All lanes:

Dot Blot - Goat Anti-Human IgG H&L (Alkaline Phosphatase) (<a href='/en-us/products/secondary-antibodies/goat-human-igg-h-l-alkaline-phosphatase-ab6859'>ab6859</a>)

Lane 1:

Human IgG at 200 ng

Lane 2:

Human IgG at 66.67 ng

Lane 3:

Human IgG at 22.2 ng

Lane 4:

Human IgG at 7.4 ng

Lane 5:

Human IgG at 2.47 ng

Secondary

All lanes:

Alkaline Phosphatase conjugated Goat anti-Human IgG antibody at 1/1000 dilution

false

Key facts

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>BCIP/NBT system is based on hydrolysis of BCIP and reduction of NBT producing a deep purple reaction product. Reaction is several times more sensitive than existing products with little or no background staining. The reaction product is extremely stable and does not fade when exposed to light.</p>" } } }

Product details

NBT / BCIP chromogen (ab7468): a sensitive substrate for alkaline phosphatase detection in immunoblotting. Ready to use formulation.

BCIP: 5-bromo-4-chloro-3-indolyl phosphate NBT: p-nitroblue tetrazolium chloride.

This product used to be called "Alkaline phosphatase detection kit (BCIP/NBT)".

NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.

To use: Wash the membrane after incubation with the alkaline phosphatase conjugate secondary reagent. Apply sufficient substrate to cover the membrane. Color (purple-turquoise, varies with pH) will develop within 30 minutes. Wash, dry membrane and image.

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Please refer to protocols

Product protocols

Target data

Additional targets

Alkaline Phosphatase Chromogen

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Arthritis research & therapy 23:113 PubMed33853645

2021

Early detection of osteoarthritis in the rat with an antibody specific to type II collagen modified by reactive oxygen species.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Gigout,Donata Harazin,Louise M Topping,Didier Merciris,Sven Lindemann,Christian Brenneis,Ahuva Nissim

PloS one 13:e0192109 PubMed29394264

2018

Epstein-Barr virus is present in the brain of most cases of multiple sclerosis and may engage more than just B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Asma Hassani,John R Corboy,Suhail Al-Salam,Gulfaraz Khan

Scientific reports 6:29406 PubMed27406241

2016

3'-UTR engineering to improve soluble expression and fine-tuning of activity of cascade enzymes in Escherichia coli.

Applications

WB

Species

Unspecified reactive species

Ji-Won Song,Ji-Min Woo,Gyoo Yeol Jung,Uwe T Bornscheuer,Jin-Byung Park
View all publications

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