NBT / BCIP chromogen (ab7468): a sensitive substrate for alkaline phosphatase detection in immunoblotting.
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- Arthritis research & therapy 23:1132021Early detection of osteoarthritis in the rat with an antibody specific to type II collagen modified by reactive oxygen species.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne Gigout,Donata Harazin,Louise M Topping,Didier Merciris,Sven Lindemann,Christian Brenneis,Ahuva NissimPubMed 33853645
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes BCIP/NBT system is based on hydrolysis of BCIP and reduction of NBT producing a deep purple reaction product. Reaction is several times more sensitive than existing products with little or no background staining. The reaction product is extremely stable and does not fade when exposed to light. |
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NBT / BCIP chromogen (ab7468): a sensitive substrate for alkaline phosphatase detection in immunoblotting.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Please refer to protocols
Notes
NBT / BCIP chromogen (ab7468): a sensitive substrate for alkaline phosphatase detection in immunoblotting. Ready to use formulation.
BCIP: 5-bromo-4-chloro-3-indolyl phosphate NBT: p-nitroblue tetrazolium chloride.
This product used to be called "Alkaline phosphatase detection kit (BCIP/NBT)".
NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.
To use: Wash the membrane after incubation with the alkaline phosphatase conjugate secondary reagent. Apply sufficient substrate to cover the membrane. Color (purple-turquoise, varies with pH) will develop within 30 minutes. Wash, dry membrane and image.
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4 product images
Dot Blot - NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (ab7468)
Dot blot analysis of Goat Anti-Human IgG H&L (Alkaline Phosphatase) ab6859. Detected using Alkaline Phosphatase Substrate (ab7468) for 30 seconds at RT.
All lanes: Dot Blot - Goat Anti-Human IgG H&L (Alkaline Phosphatase) (Goat Anti-Human IgG H&L (Alkaline Phosphatase) ab6859)
Lane 1: Human IgG at 200 ng
Lane 2: Human IgG at 66.67 ng
Lane 3: Human IgG at 22.2 ng
Lane 4: Human IgG at 7.4 ng
Lane 5: Human IgG at 2.47 ng
SecondaryAll lanes: Alkaline Phosphatase conjugated Goat anti-Human IgG antibody at 1/1000 dilution
Dot Blot - NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (ab7468)
Dot blot analysis of Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) ab6742. Detected using Alkaline Phosphatase Substrate (ab7468).
All lanes: Dot Blot - Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) ab6742)
Lane 1: Goat IgG at 200 ng
Lane 2: Goat IgG at 66.67 ng
Lane 3: Goat IgG at 22.2 ng
Lane 4: Goat IgG at 7.4 ng
Lane 5: Goat IgG at 2.47 ng
SecondaryAll lanes: Goat IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated at 1/1000 dilution
Dot Blot - NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (ab7468)
Dot blot analysis of Goat Anti-Rat IgG H&L (Alkaline Phosphatase) preadsorbed ab7098. Detected using Alkaline Phosphatase Substrate (ab7468) for 30 seconds at RT.
All lanes: Dot Blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) preadsorbed (Goat Anti-Rat IgG H&L (Alkaline Phosphatase) preadsorbed ab7098) at 1/1000 dilution
Lane 1: Rat IgG at 100 ng
Lane 2: Rat IgG at 33.3 ng
Lane 3: Rat IgG at 11.1 ng
Lane 4: Rat IgG at 3.7 ng
Lane 5: Rat IgG at 1.23 ng
Dot Blot - NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (ab7468)
Dot blot analysis of Goat Anti-Guinea pig IgG H&L (Alkaline Phosphatase) preadsorbed ab7140. Detected using Alkaline Phosphatase Substrate (ab7468) for 30 seconds at RT.
All lanes: Dot Blot - Goat Anti-Guinea pig IgG H&L (Alkaline Phosphatase) preadsorbed (Goat Anti-Guinea pig IgG H&L (Alkaline Phosphatase) preadsorbed ab7140)
Lane 1: Guinea Pig IgG at 200 ng
Lane 2: Guinea Pig IgG at 66.67 ng
Lane 3: Guinea Pig IgG at 22.2 ng
Lane 4: Guinea Pig IgG at 7.4 ng
Lane 5: Guinea Pig IgG at 2.47 ng
SecondaryAll lanes: Goat anti-Guinea Pig IgG Antibody (Min X 10) Alkaline Phosphatase Conjugated Antibody at 1/1000 dilution
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