NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen
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(3 Publications)
- Ready-to-use solution.
- Dot
Supplier Data
Dot Blot - NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (AB7468)
Dot blot analysis of ab6742. Detected using Alkaline Phosphatase Substrate (ab7468).
All lanes:
Dot Blot - Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (<a href='/en-us/products/secondary-antibodies/rabbit-goat-igg-h-l-alkaline-phosphatase-ab6742'>ab6742</a>)
Lane 1:
Goat IgG at 200 ng
Lane 2:
Goat IgG at 66.67 ng
Lane 3:
Goat IgG at 22.2 ng
Lane 4:
Goat IgG at 7.4 ng
Lane 5:
Goat IgG at 2.47 ng
Secondary
All lanes:
Goat IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated at 1/1000 dilution
false
- Dot
Supplier Data
Dot Blot - NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (AB7468)
Dot blot analysis of ab7140. Detected using Alkaline Phosphatase Substrate (ab7468) for 30 seconds at RT.
All lanes:
Dot Blot - Goat Anti-Guinea pig IgG H&L (Alkaline Phosphatase) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-guinea-pig-igg-h-l-alkaline-phosphatase-preadsorbed-ab7140'>ab7140</a>)
Lane 1:
Guinea Pig IgG at 200 ng
Lane 2:
Guinea Pig IgG at 66.67 ng
Lane 3:
Guinea Pig IgG at 22.2 ng
Lane 4:
Guinea Pig IgG at 7.4 ng
Lane 5:
Guinea Pig IgG at 2.47 ng
Secondary
All lanes:
Goat anti-Guinea Pig IgG Antibody (Min X 10) Alkaline Phosphatase Conjugated Antibody at 1/1000 dilution
false
- Dot
Supplier Data
Dot Blot - NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (AB7468)
Dot blot analysis of ab7098. Detected using Alkaline Phosphatase Substrate (ab7468) for 30 seconds at RT.
All lanes:
Dot Blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-alkaline-phosphatase-preadsorbed-ab7098'>ab7098</a>) at 1/1000 dilution
Lane 1:
Rat IgG at 100 ng
Lane 2:
Rat IgG at 33.3 ng
Lane 3:
Rat IgG at 11.1 ng
Lane 4:
Rat IgG at 3.7 ng
Lane 5:
Rat IgG at 1.23 ng
false
- Dot
Supplier Data
Dot Blot - NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (AB7468)
Dot blot analysis of ab6859. Detected using Alkaline Phosphatase Substrate (ab7468) for 30 seconds at RT.
All lanes:
Dot Blot - Goat Anti-Human IgG H&L (Alkaline Phosphatase) (<a href='/en-us/products/secondary-antibodies/goat-human-igg-h-l-alkaline-phosphatase-ab6859'>ab6859</a>)
Lane 1:
Human IgG at 200 ng
Lane 2:
Human IgG at 66.67 ng
Lane 3:
Human IgG at 22.2 ng
Lane 4:
Human IgG at 7.4 ng
Lane 5:
Human IgG at 2.47 ng
Secondary
All lanes:
Alkaline Phosphatase conjugated Goat anti-Human IgG antibody at 1/1000 dilution
false
Reactivity data
Product details
NBT / BCIP chromogen (ab7468): a sensitive substrate for alkaline phosphatase detection in immunoblotting. Ready to use formulation.
BCIP: 5-bromo-4-chloro-3-indolyl phosphate NBT: p-nitroblue tetrazolium chloride.
This product used to be called "Alkaline phosphatase detection kit (BCIP/NBT)".
NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.
To use: Wash the membrane after incubation with the alkaline phosphatase conjugate secondary reagent. Apply sufficient substrate to cover the membrane. Color (purple-turquoise, varies with pH) will develop within 30 minutes. Wash, dry membrane and image.
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Target data
Additional targets
Publications (3)
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Arthritis research & therapy 23:113 PubMed33853645
2021
Applications
Unspecified application
Species
Unspecified reactive species
PloS one 13:e0192109 PubMed29394264
2018
Applications
Unspecified application
Species
Unspecified reactive species
Scientific reports 6:29406 PubMed27406241
2016
Applications
WB
Species
Unspecified reactive species
Product promise
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