Antibody conjugation, also known as antibody labeling, chemically links an antibody to a specific tag. When conjugated, a primary antibody directly detects the target antigen of interest, without the need for a secondary antibody. Labels can include fluorescent dyes, enzymes or proteins and your choice or combination of labels will depend on the experimental application (multiplex IHC, flow cytometry, LFA, others), as well as your multiplex panel. Conjugated primary antibodies can directly detect the target antigen without the aid of secondary antibodies. Using directly labeled primary antibodies can save you a lot of time and simplify your experimental protocols.
Our Lightning-Link® conjugation kits are easy to use and allow you to conjugate antibodies in three simple steps to more than 45 labels including fluorescent proteins (Alexa Fluor® and DyLight Fluor® dyes), tandem dyes, enzymes, oligonucleotides, and gold nanoparticles. Use our fluorescent dye chart to choose the right dye to build your next multiplex IHC panel. To generate high-quality conjugates we recommend using Lightning-Link® conjugation kits to conjugate our range of carrier-free antibodies and antibody pairs specifically designed for antibody labeling. We offer 10,000 formulations free from glycerol, BSA and sodium azide to reduce antibody loss and maximize conjugation efficiency. If your antibody does not meet these buffer requirements, you can easily purify or concentrate using our range of purification and concentration kits.
Explore our range of fluorescent conjugates antibodies or learn how to generate reproducible conjugates with our antibody conjugation guide.
If you're interested in outsourcing antibody labeling to our team of experts, please get in touch and ask about our Customer Conjugation Services. Contact us today to discuss your needs.
Browse our most popular conjugation kits:
Enzymes: HRP | Alkaline Phosphatase
Proteins: Biotin | Streptavidin
Fluorophores: AF®488 | AF®647 | R-PE | APC | FITC | Cy3® | Cy5®
Tandem dyes: PE/Cy7® | APC/Cy7® | PE/Cy5® | PerCP/Cy5.5® | PE/Cy5.5®
Oligonucleotide: Universal
Metal Isotopes: 169Tm | 141Pr | 160Gd | 175Lu | 165Ho
Gold nanoparticles: 40nm | 20 nm | 10 nm