142Nd Metal Conjugation Kit - Lightning-Link®
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- Rapid Conjugation: achieve 142Nd Metal isotope labeling in under 2 hours with just 30 seconds of hands-on time.
- High Efficiency: ensures 100% antibody recovery, meaning no loss of valuable antibodies.
- Versatility: suitable for conjugating antibodies, proteins, and peptides. 142Nd metal-labeled antibodies can be used immediately without further purification.
- Schematic Diagram
Supplier Data
Schematic Diagram - 142Nd Metal Conjugation Kit - Lightning-Link® (AB269827)
This illustration demonstrates a general procedure of how Lightning-Link® labeling technology enables the direct labeling of antibodies or proteins.
Simply pipette your antibody or biomolecule of choice into the vial of a lyophilized mixture containing the label of interest and incubate for around 3 hours. Please see the ab269827 protocol booklet for more details.
Learn more about our Lightning-Link® conjugation kits here
- Conjugation
Unknown
Conjugation - 142Nd Metal Conjugation Kit - Lightning-Link® (AB269827)
Gel Image Showing Lightning-Link® Metal Labeling Kit Antibody Labeling Efficiency.
Goat Anti-Rabbit was labeled with a selection of Lightning-Link® metal labels at 10 μg single reaction vials. 2 μg conjugates per well were run on a 4-12% Bis-Tris gel, under non-reducing conditions for 20 minutes at 50V and 1 hour at 120V in 1x MOPS. The gel was stained with InstantBlue™ for 1 hour on the gel rocker. The gel showed no free antibody post conjugation for all conjugates except when the reaction was quenched at t=0, where no conjugation was observed.
Product details
142Nd Metal Conjugation Kit - Lightning-Link® (ab269827) provides an easy-to-use, one step procedure that allows researchers to label proteins, peptides and other biomolecules containing primary amines with 142Nd with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.
This product is manufactured by Expedeon, an Abcam company. Expedeon product code M142-0010 was previously called Lightning Link®-142Nd, 1 x 10 µg, 1 x 10 µg and is the same as the 10 µg size of this product. Expedeon product code M142-0100 was previously called Lightning Link®-142Nd, 1 x 100 µg, 1 x 100 µg and is the same as the 100 µg size.
The antibody to be labeled should be purified, in an appropriate buffer for conjugation and at a suitable concentration.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to 142Nd.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
| 50mM / 0.6% Tris1 | 0.1% BSA2 | 50% glycerol |
| 0.1% sodium azide | PBS | Potassium phosphate |
| Sodium chloride | HEPES | Sucrose |
| Sodium citrate | EDTA | Trehalose |
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.
Incompatible buffer constituents
| Thiomerosal | Proclin | Glycine |
| Arginine | Glutathione | DTT |
If a constituent of the buffer containing your antibody or protein is not listed above, please contact us.
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Storing and handling conjugation kits
Lyophilized Lightning-Link® components are hygroscopic.
Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com