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Labeling in < 4 hrs with 30 secs hands-on time using 490/515 nm Extracellular Vesicle Membrane Staining Kit ab314686. 100% antibody recovery.

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Images

Key facts

Detection method

Flow cytometry-fluorescent

Assay Platform

Fluorescence microscope

Sample type

Suspension cells, Adherent cells

Assay type

Cell-based (quantitative)

Reactive species

Mammals

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Reactivity data

Application

Flow Cyt

Reactivity

Reacts

Dilution info

-

Notes

-

What's included?

100 Tests
Components
490/515 EV Membrane Stain
1 x 1 Vial
Reconstitution Solution (1 mL)
1 x 1 mL

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Labeling in < 4 hrs with 30 secs hands-on time using 490/515 nm Extracellular Vesicle Membrane Staining Kit ab314686. 100% antibody recovery.

Key facts

Detection method

Flow cytometry-fluorescent

Assay Platform

Fluorescence microscope

Sample type

Suspension cells, Adherent cells

Assay type

Cell-based (quantitative)

Reactive species

Mammals

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

+4°C

Notes

490/515 nm Extracellular Vesicle Membrane Staining Kit (ab314686) was designed to overcome some of the challenges of EV detection, particularly in flow cytometry. CTB EV stains bind to molecules in the EV membrane for bright, specific staining, with little to no background.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - 490/515 nm Extracellular Vesicle Membrane Staining Kit (ab314686), expandable thumbnail

    Flow Cytometry - 490/515 nm Extracellular Vesicle Membrane Staining Kit (ab314686)

    Flow cytometry of bead-bound EVs derived from MCF-7 cells. Unstained EVs (gray) or stained with 10X 490/515 (ab314686) (green). EVs were detected on a CytoFLEX LX flow cytometer in the FITC channel.

  • Flow Cytometry - 490/515 nm Extracellular Vesicle Membrane Staining Kit (ab314686), expandable thumbnail

    Flow Cytometry - 490/515 nm Extracellular Vesicle Membrane Staining Kit (ab314686)

    SEC-purified MCF-7-derived EVs were stained with 490/515 EV Membrane Stain (ab314686) (left). Specific staining was seen, compared with the same stain in buffer (right). EVs were detected on a CytoFLEX LX flow cytometer in the FITC channel.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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