Labeling in < 4 hrs with 30 secs hands-on time using 490/515 nm Extracellular Vesicle Membrane Staining Kit ab314686. 100% antibody recovery.
Images
Key facts
- Detection method
- Flow cytometry-fluorescent
- Assay Platform
- Fluorescence microscope
- Sample type
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
- Reactive species
- Mammals
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
What's included?
Labeling in < 4 hrs with 30 secs hands-on time using 490/515 nm Extracellular Vesicle Membrane Staining Kit ab314686. 100% antibody recovery.
Key facts
- Detection method
- Flow cytometry-fluorescent
- Assay Platform
- Fluorescence microscope
- Sample type
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
- Reactive species
- Mammals
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- +4°C
Notes
490/515 nm Extracellular Vesicle Membrane Staining Kit (ab314686) was designed to overcome some of the challenges of EV detection, particularly in flow cytometry. CTB EV stains bind to molecules in the EV membrane for bright, specific staining, with little to no background.
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Flow Cytometry - 490/515 nm Extracellular Vesicle Membrane Staining Kit (ab314686)
Flow cytometry of bead-bound EVs derived from MCF-7 cells. Unstained EVs (gray) or stained with 10X 490/515 (ab314686) (green). EVs were detected on a CytoFLEX LX flow cytometer in the FITC channel.
Flow Cytometry - 490/515 nm Extracellular Vesicle Membrane Staining Kit (ab314686)
SEC-purified MCF-7-derived EVs were stained with 490/515 EV Membrane Stain (ab314686) (left). Specific staining was seen, compared with the same stain in buffer (right). EVs were detected on a CytoFLEX LX flow cytometer in the FITC channel.
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