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Oligonucleotide conjugation / labeling in < 4 hrs with 30 secs hands-on time using 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10 ab270703. 100% antibody recovery.

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What's included?

1 Kit
Components
Conjugate Resuspension Buffer
1 x 1500 µL
Modifier reagent
1 x 200 µL
Oligo 10X5001
1 x 10 µg
Oligo 10X5002
1 x 10 µg
Oligo 10X5003
1 x 10 µg
Oligo 10X5004
1 x 10 µg
Oligo 10X5005
1 x 10 µg
Oligo 10X5006
1 x 10 µg
Oligo 10X5007
1 x 10 µg
Oligo 10X5008
1 x 10 µg
Oligo 10X5009
1 x 10 µg
Oligo 10X5010
1 x 10 µg
Quencher N
1 x 200 µL
Reagent C
1 x 500 µg
Reagent S
1 x 1250 µL

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Oligonucleotide conjugation / labeling in < 4 hrs with 30 secs hands-on time using 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10 ab270703. 100% antibody recovery.

Storage

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

5' Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10 (ab270703) enables simple and rapid conjugation of oligonucleotide barcodes to antibodies to enable cell surface protein analysis in Multiomic Cytometry on 10x Genomics Chromium Controller. This kit is compatible with Chromium Single Cell Immune Profiling.

The kit includes 10 distinct oligonucleotide barcodes ready to conjugate to 10 μg of antibody each. The protocol involves only a few steps, requires minimal hands-on time and doesn't require any expertise in antibody conjugation or oligonucleotide sourcing.

Please find antibody buffer considerations and compatibility in the protocol booklet. For incompatible buffers and low antibody concentrations, please see our antibody purification and concentration kits.

For conjugating antibodies with additional distinct oligonucleotide barcodes, please see 5' Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20 (5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20 ab270705) and 5' Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 (5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 ab270709). 100 μg conjugation kits are available on-demand for each of these 30 oligonucleotide barcodes. For conjugating more than 30 antibodies with distinct oligonucleotide barcodes, please contact us to discuss your requirements.

For oligonucleotide barcode sequence information, please see appendix in the protocol booklet.

Storing and handling conjugation kits

Lyophilized Lightning-Link® components are hygroscopic.

Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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