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AB270709

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30

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5' Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 (ab270709) offers several standout features:

- Rapid Conjugation: achieve oligonucleotide barcode labeling in under 4 hours with just 30 seconds of hands-on time.
- High Efficiency: ensures 100% antibody recovery, meaning no loss of valuable antibodies.
- Versatility: suitable for conjugating antibodies, proteins, and peptides making it suitable for various applications.
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Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 (AB270709)
  • Schematic Diagram

Supplier Data

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 (AB270709)

This illustration demonstrates a general procedure of how Lightning-Link® labeling technology enables the direct labeling of antibodies or proteins.

Simply pipette your antibody or biomolecule of choice into the vial of a lyophilized mixture containing the label of interest and incubate for around 3 hours. Please see the ab270709 protocol booklet for more details.

Learn more about our Lightning-Link® conjugation kits here

Key facts

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Product details

5' Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 (ab270709) enables simple and rapid conjugation of oligonucleotide barcodes to antibodies to enable cell surface protein analysis in Multiomic Cytometry on 10x Genomics Chromium Controller. This kit is compatible with Chromium Single Cell Immune Profiling.

The kit includes 10 distinct oligonucleotide barcodes ready to conjugate to 10 μg of antibody each. The protocol involves only a few steps, requires minimal hands-on time and doesn't require any expertise in antibody conjugation or oligonucleotide sourcing.

Please find antibody buffer considerations and compatibility in the protocol booklet. For incompatible buffers and low antibody concentrations, please see our antibody purification and concentration kits.

For conjugating antibodies with additional distinct oligonucleotide barcodes, please see 5' Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10 (ab270703) and 5' Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20 (ab270705). 100 μg conjugation kits are available on-demand for each of these 30 oligonucleotide barcodes. For conjugating more than 30 antibodies with distinct oligonucleotide barcodes, please contact us to discuss your requirements.

For oligonucleotide barcode sequence information, please see appendix in the protocol booklet.

Storing and handling conjugation kits

Lyophilized Lightning-Link® components are hygroscopic.

Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.

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What's included?

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Properties and storage information

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com