Labeling in < 4 hrs with 30 secs hands-on time using 560/585 nm Extracellular Vesicle Membrane Staining Kit ab314687. 100% antibody recovery.
Application | Reactivity | Dilution info | Notes |
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Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
Labeling in < 4 hrs with 30 secs hands-on time using 560/585 nm Extracellular Vesicle Membrane Staining Kit ab314687. 100% antibody recovery.
560/585 nm Extracellular Vesicle Membrane Staining Kit (ab314687) was designed to overcome some of the challenges of EV detection, particularly in flow cytometry. CTB EV stains bind to molecules in the EV membrane for bright, specific staining, with little to no background.
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SEC-purified MCF-7-derived EVs were stained with 560/585 EV Membrane Stain (ab314687) (left). Specific staining was seen, compared with the same stain in buffer (right). EVs were detected on a CytoFLEX LX flow cytometer in the PE channel.
Flow cytometry of bead-bound EVs derived from MCF-7 cells. Unstained EVs (gray) or stained with 10X 560/585 (ab314687) (orange). EVs were detected on a CytoFLEX LX flow cytometer in the R-PE channel.
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