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Alexa Fluor® 555 conjugation / labeling in < 20 mins with 30 secs hands-on time using Alexa Fluor® 555 Conjugation Kit (Fast) - Lightning-Link® ab269820. 100% antibody recovery.


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What's included?

3 x 10 µg
Components
Alexa Fluor 555® mix (Lyophilized)
3 x 10 µg
Modifier reagent
1 x 200 µL
Quencher reagent
1 x 200 µL

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Alexa Fluor® 555 conjugation / labeling in < 20 mins with 30 secs hands-on time using Alexa Fluor® 555 Conjugation Kit (Fast) - Lightning-Link® ab269820. 100% antibody recovery.

Storage

Shipped at conditions

Ambient - Cannot Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Alexa Fluor® 555 Conjugation Kit - Lightning-Link® (ab269820) provides an easy-to-use, one step procedure that allows researchers to covalently label proteins, peptides and other biomolecules containing primary amines with Alexa Fluor® 555 with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes. The antibody to be labeled should be purified, in an appropriate buffer for conjugation and at a suitable concentration. Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid . Use the to learn more about the technology, or about conjugating other proteins and peptides to Alexa Fluor® 555. Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.

This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Rapid Alexa Fluor® 555 Labeling Kit. 333-0015 is the same as the 1 mg size. 333-0010 is the same as the 3 x 100 ug size. 333-0030 is the same as the 3 x 10 ug size. 333-0005 is the same as the 100 μg size.

Amount and volume of antibody for conjugation to Alexa Fluor® 555

Kit sizeRecommended
amount of antibody1
Maximum
amount of antibody
Maximum antibody
volume2
3 x 10 μg3 x 10 μg3 x 20 μg3 x 10 μL
100 μg100 μg200 μg100 μL
3 x 100 μg3 x 100 μg3 x 200 μg3 x 100 μL
1 mg1 x 1 mg1 x 2 mg1 x 1 mL

1 Using the maximum amount of antibody may result in less labelling per antibody.

2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2mg/ml or < 0.5 mg/ml should be diluted /concentrated.

Buffer Requirements for Conjugation

Buffer should be pH 6.5-8.5.

Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

50mM / 0.6% Tris10.1% BSA250% glycerol
0.1% sodium azidePBSPotassium phosphate
Sodium chlorideHEPESSucrose
Sodium citrateEDTATrehalose

1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.

Incompatible buffer constituents

ThiomerosalProclinGlycine
ArginineGlutathioneDTT

If a constituent of the buffer containing your antibody or protein is not listed above, please contact us.

Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

Storing and handling conjugation kits

Lyophilized Lightning-Link® components are hygroscopic.

Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Conjugation - Alexa Fluor® 555 Conjugation Kit (Fast) - Lightning-Link® (ab269820), expandable thumbnail

    Conjugation - Alexa Fluor® 555 Conjugation Kit (Fast) - Lightning-Link® (ab269820)

    AlexaFluor 555 spectra.

    AlexaFluor® 555 conjugate absorbance and emission spectra.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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