Alkaline Phosphatase Conjugation Kit (Animal Free) - Lightning-Link®
Be the first to review this product! Submit a review
|
(0 Publication)
- Rapid Conjugation: achieve Alkaline Phosphatase (AP) labeling in under 4 hours with just 30 seconds of hands-on time.
- Animal-free alternative to ab102850.
- High Efficiency: ensures 100% antibody recovery, meaning no loss of valuable antibodies.
- Versatility: suitable for conjugating antibodies, proteins, and peptides. AP-labeled antibodies can be used immediately in applications such as Western blot, ELISA, and Immunohistochemistry (IHC) without further purification.
- Schematic Diagram
Supplier Data
Schematic Diagram - Alkaline Phosphatase Conjugation Kit (Animal Free) - Lightning-Link® (AB269901)
This illustration demonstrates a general procedure of how Lightning-Link® labeling technology enables the direct labeling of antibodies or proteins.
Simply pipette your antibody or biomolecule of choice into the vial of a lyophilized mixture containing the label of interest and incubate for around 3 hours. Please see the ab269901 protocol booklet for more details.
Learn more about our Lightning-Link® conjugation kits here
- Conjugation
Supplier Data
Conjugation - Alkaline Phosphatase Conjugation Kit (Animal Free) - Lightning-Link® (AB269901)
Standard curve.
pNPP detection of an Alkaline Phosphatase conjugate.
Product details
Alkaline Phosphatase (Animal Free) Conjugation Kit / Alkaline Phosphatase Labeling Kit ab236901 uses a simple and quick process for alkaline phosphatase labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.
The alkaline phosphatase used in this kit is harvested from microbial sources and provides an animal friendly alternative to conventional alkaline phosphatase products sourced from bovine intestine.
This product is manufactured by Expedeon, an Abcam company. See the table below to match Abcam kit size against Expedeon product code.
To conjugate an antibody to alkaline phosphatase using this kit:- add modifier to antibody and incubate for 3 hours
- add quencher and incubate for 30 mins
The alkaline phosphatase conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to alkaline phosphatase.
Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.
This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Alkaline Phosphatase AF (Animal Free) Labeling Kit. 798-0015 is the same as the 1 mg size. 798-0030 is the same as the 3 x 10 ug size. 798-0010 is the same as the 3 x 100 ug size. 798-0005 is the same as the 100 μg size.
Amount and volume of antibody for conjugation to Alkaline Phosphatase
| Kit size | Recommended amount of antibody1 |
Maximum amount of antibody |
Maximum antibody volume2 |
| 3 x 10 μg | 3 x 10 μg | 3 x 20 μg | 3 x 10 μL |
| 100 μg | 100 μg | 200 μg | 100 μL |
| 3 x 100 μg | 3 x 100 μg | 3 x 200 μg | 3 x 100 μL |
| 1 mg | 1 x 1 mg | 1 x 2 mg | 1 x 1 mL |
1 Using the maximum amount of antibody may result in less labelling per antibody.
2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 1 mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
| 50mM / 0.6% Tris1 | 0.1% BSA2 | 50% glycerol |
| 0.1% sodium azide | PBS | Potassium phosphate |
| Sodium chloride | HEPES | Sucrose |
| Sodium citrate | EDTA | Trehalose |
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.
Incompatible buffer constituents
| Thiomerosal | Proclin | Glycine |
| Arginine | Glutathione | DTT |
If a constituent of the buffer containing your antibody or protein is not listed above, please contact us.
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Storing and handling conjugation kits
Lyophilized Lightning-Link® components are hygroscopic.
Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
- Download websiteProtocolBooklet|zh
Target data
Complementary Products
Sample Prep & Detection Kits
AB201796
Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link®
- 3
- 1
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com