Antibody Biotinylation Check Kit

Product details

Antibody Biotinylation Check Kit (ab270030) is a quick immunochromatography test that allows you to confirm the successful antibody biotinylation in one easy step, and without the need for any specialized or costly equipment. Unlike traditional methods of assessing the success of biotinylation, such as HABA assays, which can be tedious and time consuming, this kit requires only 10 minutes to perform and very small quantities of your antibody conjugate.

The key component of the kit is a nitrocellulose membrane containing a Test line of immobilized Protein A and Protein G with high affinity for the Fc region of a variety of IgG molecules, and a 40 nm Gold-Streptavidin conjugate for detection. When the biotinylated antibody is mixed with the Gold-Streptavidin conjugate, it forms a complex that flows along the nitrocellulose membrane and binds to the Protein A and Protein G on the Test line. A visible red line appears on the strip confirming successful conjugation.

Benefits:

Quick and easy qualitative test to check success of conjugation

Compatible with antibody conjugates generated with Lightning-Link® Biotin conjugation kits (ab201795 and ab201796)

No costly equipment required

Small volumes of conjugate required (20 μl)

General notes:

This product is manufactured by Expedeon, an Abcam company. It was previously called Biotin Check&Go! Antibody Labeling Confirmation Kit. Expedeon product code 4001-0030 is the same as the 30-test size of this product.