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AB312830

APC-iFluor® 700 Conjugation Kit

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APC-iFluor® 700 conjugation / labeling in < 4 hrs with 30 secs hands-on time using APC-iFluor® 700 Conjugation Kit AB312830. 100% antibody recovery.
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Flow Cytometry - APC-iFluor® 700 Conjugation Kit (AB312830)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC-iFluor® 700 Conjugation Kit (AB312830)

Flow cytometry analysis of whole blood stained with APC-iFluor® 700 anti-human CD4 *SK3* conjugate prepared using the iAPC-iFluor® 700 Conjugation Kit (ab312830 & ab312831). The fluorescence signal was monitored using an Aurora flow cytometer in the APC-iFluor® 700-specific R4-A channel.

Flow Cytometry - APC-iFluor® 700 Conjugation Kit (AB312830)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC-iFluor® 700 Conjugation Kit (AB312830)

Flow cytometric analysis of APC-iFluor® 700 (Red Bar) or APC-Alexa Fluor® 700 (Blue Bar) anti-human CD8 on human lymphocytes. Whole blood was stained with ab312830 or APC-Alexa Fluor® 700 anti-human CD8 and compared to whole blood stained with a APC-iFluor® 700 and APC-Alexa Fluor® 700 mouse IgG control. Flow cytometry was performed on a ACEA flow cytometry system.

Flow Cytometry - APC-iFluor® 700 Conjugation Kit (AB312830)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC-iFluor® 700 Conjugation Kit (AB312830)

Flow cytometry analysis of PBMC stained with APC-iFluor® 700 anti-human CD4 *RPA-T4* conjugate. The fluorescence signal was monitored using an Aurora flow cytometer in the APC-iFluor® 700 specific R4-A channel.

Key facts

Detection method

Fluorescent

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Product details

APC-iFluor® 700 Conjugation Kit provides a convenient method for labelling small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the Red Laser-excitable XFD750. This dye has a fluorescence maxima (Ex/Em) of 651/713 nm respectively. These characteristics make APC-iFluor® 700 Tandem an excellent replacement for APC-Alexa Fluor® 700 Tandem, since they have almost identical spectra. APC-iFluor® 700 Tandem is a stable dye and shows good reactivity and selectivity with protein amino groups.

This conjugation kit has all the essential components to perform two separate labelling reactions with no column purification needed. Each of the two vials of APC-iFluor® 700 Tandem provided in the kit is optimized for labelling ~50 μg antibody.

Impure antibodies or antibodies stabilized with bovine serum albumin (BSA) or gelatin will not be labelled well, Abcam offers a range of purification kits

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What's included?

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

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