Atto 390 Conjugation Kit (Fast) - Lightning-Link®
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- Rapid Conjugation: achieve Atto 390 labeling in under 2 hours with just 30 seconds of hands-on time.
- High Efficiency: ensures 100% antibody recovery, meaning no loss of valuable antibodies.
- Versatility: suitable for conjugating antibodies, proteins, and peptides. Atto 390-labeled antibodies can be used immediately in applications such as Western blot, Flow cytometry, ELISA, and Immunohistochemistry (IHC) without further purification.
- Schematic Diagram
Supplier Data
Schematic Diagram - Atto 390 Conjugation Kit (Fast) - Lightning-Link® (AB269895)
This illustration demonstrates a general procedure of how the Lightning-Link®(Fast) labeling technology enables the direct labeling of antibodies or proteins.
Simply pipette your antibody or biomolecule of choice into the vial of a lyophilized mixture containing the label of interest and incubate for just 15 minutes. Please see the ab269895 protocol booklet for more details.
Learn more about our Lightning-Link® conjugation kits here
- Flow Cyt
PubMed
Flow Cytometry - Atto 390 Conjugation Kit (Fast) - Lightning-Link® (AB269895)
Taichman, Russell S., et al used Atto 390 Conjugation Kit (Fast) - Lightning-Link® (ab269895) as part of examining Axl and Tyro3 expression during experimental prostate cancer (PCa) progression. They used the kit to conjugate Atto 390 to antibodies for use in flow cytometry.
Anti-Axl, anti-Tyro3 and anti-Ki67 antibodies were conjugated to the fluorophores APC-Cy7, PE-Cy5, and Atto390 using our Lightning-Link® Conjugation kits. (A) Experimental model. Human PCa cell lines (PC3Luc, DU145Luc) were implanted s.c. into male SCID mice as a model of a primary (1°) tumor development, and removed after 1 month. At monthly intervals thereafter human PCa cells were identified by anti-HLA staining; proliferative status (Ki67 staining) and Axl or Tyro3 levels were evaluated by FACS. (B) Percent expression of Ki67 by lineage depleted (Lin-) marrow cells or by primary tumor cells at 1 month. (C-D) Percent expression of Axl or Tyro3 by primary tumor cells established with (C) DU145 or (D) PC3 cells or by DTCs recovered from marrow over time.
Image from Taichman, Russell S., et al., PloS one, 8(4): e61873. doi: 10.1371/journal.pone.0061873. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
Product details
Atto 390 Conjugation Kit / Atto 390 Labeling Kit ab269895 uses a simple and quick process for Atto 390 labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.
To conjugate an antibody to Atto 390 using this kit:
- add modifier to antibody and incubate for 15 mins
- add quencher and incubate for 5 mins
The Atto 390 conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to Atto 390.
Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.
This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Rapid Atto 390 Labeling Kit. 349-0005 is the same as the 100 μg size. 349-0010 is the same as the 3 x 100 ug size. 349-0030 is the same as the 3 x 10 ug size.
Amount and volume of antibody for conjugation to Atto 390
| Kit size | Recommended amount of antibody1 |
Maximum amount of antibody |
Maximum antibody volume2 |
| 3 x 10 μg | 3 x 10 μg | 3 x 20 μg | 3 x 10 μL |
| 100 μg | 1 x 100 μg | 1 x 200 μg | 1 x 100 μL |
| 3 x 100 μg | 3 x 100 μg | 3 x 200 μg | 3 x 100 μL |
1 Using the maximum amount of antibody may result in less labeling per antibody.
2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2 mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
| 50mM / 0.6% Tris1 | 0.1% BSA2 | 50% glycerol |
| 0.1% sodium azide | PBS | Potassium phosphate |
| Sodium chloride | HEPES | Sucrose |
| Sodium citrate | EDTA | Trehalose |
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.
Incompatible buffer constituents
| Thiomerosal | Proclin | Glycine |
| Arginine | Glutathione | DTT |
If a constituent of the buffer containing your antibody or protein is not listed above, please contact us.
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Storing and handling conjugation kits
Lyophilized Lightning-Link® components are hygroscopic.
Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
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Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
PloS one 8:e61873 PubMed23637920
2013
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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