Atto 633 Conjugation Kit (Fast) - Lightning-Link®

Product details

Atto 633 Conjugation Kit (Fast) - Lightning-Link^® (ab269898) provides an easy-to-use, one step procedure that allows researchers to covalently label proteins, peptides and other biomolecules containing primary amines with Atto 633 with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.

The antibody to be labeled should be purified, in an appropriate buffer for conjugation and at a suitable concentration.

The excitation and emmision wavelengths for Atto 633 are Ex: 630nm, Em: 651nm.

Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to Atto 633.

Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.

This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link^® Rapid Atto 633 Labeling Kit. 353-0015 is the same as the 1 mg size. 353-0010 is the same as the 3 x 100 ug size. 353-0030 is the same as the 3 x 10 ug size. 353-0005 is the same as the 100 μg size.

Amount and volume of antibody for conjugation to Atto 633

¹ Using the maximum amount of antibody may result in less labeling per antibody.

² Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2mg/ml or < 0.5 mg/ml should be diluted /concentrated.

Buffer Requirements for Conjugation

Buffer should be pH 6.5-8.5.

Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

¹ Tris buffered saline is almost always ≤ 50 mM / 0.6%
² BSA can also interfere with the use of the conjugated antibody in tissue staining.

Incompatible buffer constituents

If a constituent of the buffer containing your antibody or protein is not listed above, please contact us.

Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

Storing and handling conjugation kits

Lyophilized Lightning-Link^® components are hygroscopic.

Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.