Biotin Gold Conjugate (40nm, 10 OD)
Be the first to review this product! Submit a review
|
(0 Publication)
- Possible to exchange the Biotin-Gold (40 nm) nanoparticles into a specific buffer for your assay.
- Available in different sizes to fit your experimental needs.
- FuncS
Unknown
Functional Studies - Biotin Gold Conjugate (40nm, 10 OD) (AB186922)
Gold-Biotin demonstrates enhanced sensitivity compared to competitor products in lateral flow assays.
Comparison of test and control line signal intensities between biotin conjugated gold nanoparticles from Competitor 1 or 2 and covalently conjugated Gold-Biotin on lateral flow test strips.
- Schematic Diagram
Unknown
Schematic Diagram - Biotin Gold Conjugate (40nm, 10 OD) (AB186922)
A Ideal concentration of Streptavidin conjugated Rabbit IgG (5-50 μg/ml) binds to the GOLD-Biotin producing a strong signal on the test line (Goat anti-rabbit IgG).
B Excess Streptavidin conjugated Rabbit IgG (>400 μg/ml) results in competition between the unbound Streptavidin conjugated Rabbit IgG and GOLD-Biotin.
C A lack of Streptavidin conjugated Rabbit IgG (<10 μg/ml) leads to a weak test line.
Product details
Biotin Gold Conjugate (40nm, 10 OD) (ab186922) is a unique coated nanoparticle that has an avidly bound protective surface coat which can withstand the most extreme conditions (e.g. 2.5M NaOH at 70°C for ~2 hours).
By covalently attaching biotin ligand to the surface coat the resulting Gold-Biotin nanoparticles inherit the stability attributes of the core nanoparticle; moreover the biotin ligand cannot desorb, which is a major problem with all competing products in which a biotin analogue is directly attached to the metal. Biotin is attached to Gold via an extended linker to facilitate high affinity molecular interactions with avidin and streptavidin.
A common application for Gold Biotin is to be used as a detector in Lateral Flow Assays (LFAs).
Notes:
This product is manufactured by Expedeon, an Abcam company. It was previously called 40nm InnovaCoat® GOLD (10 OD) Biotin Conjugate. Expedeon product code 240-0200 is the same as the 200 μL size of this product and code 240-1000 is the same as the 1 mL size.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com